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Microbial glycan microarrays define key features of host-microbial interactions

Academic Article
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Overview

authors

  • Stowell, S. R.
  • Arthur, C. M.
  • McBride, R.
  • Berger, O.
  • Razi, N.
  • Heimburg-Molinaro, J.
  • Rodrigues, L. C.
  • Gourdine, J. P.
  • Noll, A. J.
  • von Gunten, S.
  • Smith, D. F.
  • Knirel, Y. A.
  • Paulson, James
  • Cummings, R. D.

publication date

  • June 2014

journal

  • Nature Chemical Biology  Journal

abstract

  • Genomic approaches continue to provide unprecedented insight into the microbiome, yet host immune interactions with diverse microbiota can be difficult to study. We therefore generated a microbial microarray containing defined antigens isolated from a broad range of microbial flora to examine adaptive and innate immunity. Serological studies with this microarray show that immunoglobulins from multiple mammalian species have unique patterns of reactivity, whereas exposure of animals to distinct microbes induces specific serological recognition. Although adaptive immunity exhibited plasticity toward microbial antigens, immunological tolerance limits reactivity toward self. We discovered that several innate immune galectins show specific recognition of microbes that express self-like antigens, leading to direct killing of a broad range of Gram-negative and Gram-positive microbes. Thus, host protection against microbes seems to represent a balance between adaptive and innate immunity to defend against evolving antigenic determinants while protecting against molecular mimicry.

subject areas

  • Adaptive Immunity
  • Animals
  • Antigens, Bacterial
  • Binding Sites
  • CHO Cells
  • Cell Survival
  • Cricetinae
  • Cricetulus
  • Fluorometry
  • Galectins
  • Gram-Negative Bacteria
  • Gram-Positive Bacteria
  • Host-Pathogen Interactions
  • Humans
  • Immunity, Innate
  • Immunoglobulin G
  • Immunoglobulin M
  • Mice
  • Microarray Analysis
  • Polysaccharides
  • Rabbits
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Identity

PubMed Central ID

  • PMC4158828

International Standard Serial Number (ISSN)

  • 1552-4450

Digital Object Identifier (DOI)

  • 10.1038/nchembio.1525

PubMed ID

  • 24814672
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Additional Document Info

start page

  • 470

end page

  • 476

volume

  • 10

issue

  • 6

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