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Sigma receptor 1 modulates ER stress and Bcl2 in murine retina

Academic Article
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Overview

authors

  • Ha, Y. J.
  • Shanmugam, A. K.
  • Markand, S.
  • Zorrilla, Eric
  • Ganapathy, V.
  • Smith, S. B.

publication date

  • April 2014

journal

  • Cell and Tissue Research  Journal

abstract

  • Sigma receptor 1 (σR1), a non-opiate transmembrane protein located on endoplasmic reticulum (ER) and mitochondrial membranes, is considered to be a molecular chaperone. Marked protection against cell death has been observed when ligands for σR1 have been used in in vitro and in vivo models of retinal cell death. Mice lacking σR1 (σR1(-/-)) manifest late-onset loss of retinal ganglion cells and retinal electrophysiological changes (after many months). The role of σR1 in the retina and the mechanisms by which its ligands afford neuroprotection are unclear. We therefore used σR1(-/-) mice to investigate the expression of ER stress genes (BiP/GRP78, Atf6, Atf4, Ire1α) and proteins involved in apoptosis (BCL2, BAX) and to examine the retinal transcriptome at young ages. Whereas no significant changes occurred in the expression of major ER stress genes (over a period of a year) in neural retina, marked changes were observed in these genes, especially Atf6, in isolated retinal Müller glial cells. BCL2 levels decreased in σR1(-/-) retina concomitantly with decreases in NFkB and pERK1/2. We postulate that σR1 regulates ER stress in retinal Müller cells and that the role of σR1 in retinal neuroprotection probably involves BCL2 and some of the proteins that modify its expression (such as ERK, NFκB). Data from the analysis of the retinal transcriptome of σR1 null mice provide new insights into the role of σR1 in retinal neuroprotection.

subject areas

  • Animals
  • Blotting, Western
  • Brain
  • Endoplasmic Reticulum Stress
  • Ependymoglial Cells
  • Extracellular Signal-Regulated MAP Kinases
  • Female
  • Gene Expression Regulation
  • Heat-Shock Proteins
  • Inositol 1,4,5-Trisphosphate Receptors
  • Mice
  • Mice, Inbred C57BL
  • NF-kappa B
  • Proto-Oncogene Proteins c-bcl-2
  • RNA, Messenger
  • Real-Time Polymerase Chain Reaction
  • Receptors, sigma
  • Retina
  • Time Factors
  • Transcriptome
  • alpha-Crystallin B Chain
  • bcl-2-Associated X Protein
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Research

keywords

  • Endoplasmic reticulum stress
  • Mouse
  • Muller cells
  • Retinal disease
  • Retinal neuroprotection
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Identity

PubMed Central ID

  • PMC3976706

International Standard Serial Number (ISSN)

  • 0302-766X

Digital Object Identifier (DOI)

  • 10.1007/s00441-013-1774-8

PubMed ID

  • 24469320
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Additional Document Info

start page

  • 15

end page

  • 27

volume

  • 356

issue

  • 1

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