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Lipidic cubic phase injector facilitates membrane protein serial femtosecond crystallography

Academic Article
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Overview

related to degree

  • Wang, Chong, Ph.D. in Chemistry, Scripps Research , transferred from UCSD 2011 - 2014
  • Wacker, Daniel, Ph.D. in Biology, Scripps Research 2009 - 2013

authors

  • Weierstall, U.
  • James, D.
  • Wang, Chong
  • White, T. A.
  • Wang, D.
  • Liu, W.
  • Spence, J. C. H.
  • Doak, R. B.
  • Nelson, G.
  • Fromme, P.
  • Fromme, R.
  • Grotjohann, I.
  • Kupitz, C.
  • Zatsepin, N. A.
  • Liu, H.
  • Basu, S.
  • Wacker, Daniel
  • Han, G. W.
  • Katritch, Vsevolod
  • Boutet, S.
  • Messerschmidt, M.
  • Williams, G. J.
  • Koglin, J. E.
  • Seibert, M. M.
  • Klinker, M.
  • Gati, C.
  • Shoeman, R. L.
  • Barty, A.
  • Chapman, H. N.
  • Kirian, R. A.
  • Beyerlein, K. R.
  • Stevens, Raymond
  • Li, D.
  • Shah, S. T. A.
  • Howe, N.
  • Caffrey, M.
  • Cherezov, Vadim

publication date

  • February 2014

journal

  • Nature Communications  Journal

abstract

  • Lipidic cubic phase (LCP) crystallization has proven successful for high-resolution structure determination of challenging membrane proteins. Here we present a technique for extruding gel-like LCP with embedded membrane protein microcrystals, providing a continuously renewed source of material for serial femtosecond crystallography. Data collected from sub-10-μm-sized crystals produced with less than 0.5 mg of purified protein yield structural insights regarding cyclopamine binding to the Smoothened receptor.

subject areas

  • Crystallography
  • Lipids
  • Membrane Proteins
  • Receptors, G-Protein-Coupled
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Identity

PubMed Central ID

  • PMC4061911

International Standard Serial Number (ISSN)

  • 2041-1723

Digital Object Identifier (DOI)

  • 10.1038/ncomms4309

PubMed ID

  • 24525480
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Additional Document Info

start page

  • 3309

volume

  • 5

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