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Ward, Andrew
Faculty Member
Graduate Student
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Positions
- Professor, Integrative Structural and Computational Biology (ISCB) , Scripps Research 2017 -
- Faculty Member, Skaggs Graduate School of Chemical and Biological Sciences , Scripps Research 2010 -
- Associate Professor, Integrative Structural and Computational Biology (ISCB) , Scripps Research 2015 - 2017
- Assistant Professor, Integrative Structural and Computational Biology (ISCB) , Scripps Research 2013 - 2015
- Assistant Professor, Molecular Biology , Scripps Research 2010 - 2012
- Research Associate with Dr. Geoffrey Chang, Molecular Biology , Scripps Research 2008 - 2010
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Publications
recent publications
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academic article
- Bangaru, S., Zhang, H., Gilchuk, I. M., Voss, T. G., Irving, R. P., Gilchuk, P., Matta, P., Zhu, X., Lang, S., Nieusma, T., Richt, J. A., Albrecht, R. A., et al. A multifunctional human monoclonal neutralizing antibody that targets a unique conserved epitope on influenza HA Nature Communications 2018 9 DOI:10.1038/s41467-018-04704-9 PMID:29991715 PMCID:PMC6039445
- Flyak, A. I., Kuzmina, N., Murin, C. D., Bryan, C., Davidson, E., Gilchuk, P., Gulka, C. P., Ilinykh, P. A., Shen, X. L., Huang, K., Ramanathan, P., Turner, H., et al. Broadly neutralizing antibodies from human survivors target a conserved site in the Ebola virus glycoprotein HR2-MPER region Nature Microbiology 2018 3:670-677 DOI:10.1038/s41564-018-0157-z PMID:29736037
- Bale, S., Martine, A., Wilson, R., Behrens, A. J., Le Fourn, V., de Val, N., Sharma, S. K., Tran, K., Torres, J. L., Girod, P. A., Ward, A. B., Crispin, M., et al. Cleavage -Independent HIV-1 Trimers From CHO Cell Lines Elicit Robust Autologous Tier 2 Neutralizing Antibodies Frontiers in Immunology 2018 9 DOI:10.3389/fimmu.2018.01116 PMID:29881382 PMCID:PMC5976746
- Rantalainen, K., Berndsen, Z. T., Murrell, S., Cao, L., Omorodion, O., Torres, J. L., Wu, M., Umotoy, J., Copps, J., Poignard, P., Landais, E., Paulson, J. C., et al. Co-evolution of HIV envelope and apex-targeting neutralizing antibody lineage provides benchmarks for vaccine design Cell Reports 2018 23:3249-3261 DOI:10.1016/j.celrep.2018.05.046 PMID:29898396 PMCID:PMC6019700
- Cao, L. W., Pauthner, M., Andrabi, R., Rantalainen, K., Berndsen, Z., Diedrich, J. K., Menis, S., Sok, D., Bastidas, R., Park, S. K. R., Delahunty, C. M., He, L., et al. Differential processing of HIV envelope glycans on the virus and soluble recombinant trimer Nature Communications 2018 9 DOI:10.1038/s41467-018-06121-4 PMID:30209313 PMCID:PMC6135743
- Ozorowski, G., Cupo, A., Golabek, M., LoPiccolo, M., Ketas, T. A., Cavallary, M., Cottrell, C. A., Klasse, P. J., Ward, A. B., Moore, J. P. Effects of adjuvants on HIV-1 envelope glycoprotein SOSIP trimers in vitro Journal of Virology 2018 92 DOI:10.1128/jvi.00381-18 PMID:29669838 PMCID:PMC6002727
- Bianchi, M., Turner, H. L., Nogal, B., Cottrell, C. A., Oyen, D., Pauthner, M., Bastidas, R., Nedellec, R., McCoy, L. E., Wilson, I. A., Burton, D. R., Ward, A. B., et al. Electron-microscopy-based epitope mapping defines specificities of polyclonal antibodies elicited during HIV-1 BG505 envelope trimer immunization Immunity 2018 49:288-300 DOI:10.1016/j.immuni.2018.07.009 PMID:30097292 PMCID:PMC6104742
- Shrivastava, T., Samal, S., Tyagi, A. K., Goswami, S., Kumar, N., Ozorowski, G., Ward, A. B., Chakrabarti, B. K. Envelope proteins of two HIV-1 clades induced different epitope-specific antibody response Vaccine 2018 36:1627-1636 DOI:10.1016/j.vaccine.2018.01.081 PMID:29429810
- Klasse, P. J., Ketas, T. J., Cottrell, C. A., Ozorowski, G., Debnath, G., Camara, D., Francomano, E., Pugach, P., Ringe, R. P., LaBranche, C. C., van Gils, M. J., Bricault, C. A., et al. Epitopes for neutralizing antibodies induced by HIV-1 envelope glycoprotein BG505 SOSIP trimers in rabbits and macaques PLoS Pathogens 2018 14 DOI:10.1371/journal.ppat.1006913 PMID:29474444 PMCID:PMC5841823
- Maurer, M. A., Meyer, L., Bianchi, M., Turner, H. L., Le, N. P. L., Steck, M., Wyrzucki, A., Orlowski, V., Ward, A. B., Crispin, M., Hangartner, L. Glycosylation of human IgA directly inhibits influenza A and other sialic-acid-binding viruses Cell Reports 2018 23:90-99 DOI:10.1016/j.celrep.2018.03.027 PMID:29617676
- Behrens, A. J., Kumar, A., Medina-Ramirez, M., Cupo, A., Marshall, K., Portillo, V. M. C., Harvey, D. J., Ozorowski, G., Zitzmann, N., Wilson, I. A., Ward, A. B., Struwe, W. B., et al. Integrity of glycosylation processing of a glycan-depleted trimeric HIV-1 immunogen targeting key B-cell lineages Journal of Proteome Research 2018 17:987-999 DOI:10.1021/acs.jproteome.7b00639 PMID:29420040 PMCID:PMC5846105
- Gilchuk, P., Kuzmina, N., Ilinykh, P. A., Huang, K., Gunn, B. M., Bryan, A., Davidson, E., Doranz, B. J., Turner, H. L., Fusco, M. L., Bramble, M. S., Hoff, N. A., et al. Multifunctional pan-ebolavirus antibody recognizes a site of broad vulnerability on the ebolavirus glycoprotein Immunity 2018 49:363-374 DOI:10.1016/j.immuni.2018.06.018 PMID:30029854 PMCID:PMC6104738
- Aldon, Y., McKay, P. F., Allen, J., Ozorowski, G., Levai, R. F., Tolazzi, M., Rogers, P., He, L., de Val, N., Fabian, K., Scarlatti, G., Zhu, J., et al. Rational design of DNA-expressed stabilized native-like HIV-1 envelope trimers Cell Reports 2018 24:3324-3338 DOI:10.1016/j.celrep.2018.08.051 PMID:30232012 PMCID:PMC6167709
- Steinhardt, J. J., Guenaga, J., Turner, H. L., McKee, K., Louder, M. K., O'Dell, S., Chiang, C. I., Lei, L., Galkin, A., Andrianov, A. K., Doria-Rose, N. A., Bailer, R. T., et al. Rational design of a trispecific antibody targeting the HIV-1 Env with elevated anti-viral activity Nature Communications 2018 9 DOI:10.1038/s41467-018-03335-4 PMID:29491415 PMCID:PMC5830440
- de Taeye, S. W., de la Pena, A. T., Vecchione, A., Scutigliani, E., Sliepen, K., Burger, J. A., van der Woude, P., Schorcht, A., Schermer, E. E., van Gils, M. J., LaBranche, C. C., Montefiori, D. C., et al. Stabilization of the gp120 V3 loop through hydrophobic interactions reduces the immunodominant V3-directed non-neutralizing response to HIV-1 envelope trimers Journal of Biological Chemistry 2018 293:1688-1701 DOI:10.1074/jbc.RA117.000709 PMID:29222332 PMCID:PMC5798299
- Schiffner, T., Pallesen, J., Russell, R. A., Dodd, J., de Val, N., LaBranche, C. C., Montefiori, D., Tomaras, G. D., Shen, X., Harris, S. L., Moghaddam, A. E., Kalyuzhniy, O., et al. Structural and immunologic correlates of chemically stabilized HIV-1 envelope glycoproteins PLoS Pathogens 2018 14 DOI:10.1371/journal.ppat.1006986 PMID:29746590 PMCID:PMC5944921
- Murin, C. D., Bruhn, J. F., Bornholdt, Z. A., Copps, J., Stanfield, R., Ward, A. B. Structural basis of pan-Ebolavirus neutralization by an antibody targeting the glycoprotein fusion loop Cell Reports 2018 24:2723-2732.e4 DOI:10.1016/j.celrep.2018.08.009 PMID:30184505
- Crispin, M., Ward, A. B., Wilson, I. A. Structure and immune recognition of the HIV glycan shield Annual Review of Biophysics 2018 47:499-523 DOI:10.1146/annurev-biophys-060414-034156 PMID:29595997
- Sarkar, A., Bale, S., Behrens, A. J., Kumar, S., Sharma, S. K., de Val, N., Pallesen, J., Irimia, A., Diwanji, D. C., Stanfield, R. L., Ward, A. B., Crispin, M., et al. Structure of a cleavage-independent HIV Env recapitulates the glycoprotein architecture of the native cleaved trimer Nature Communications 2018 9 DOI:10.1038/s41467-018-04272-y PMID:29769533 PMCID:PMC5955915
- Kefauver, J. M., Saotome, K., Dubin, A. E., Pallesen, J., Cottrell, C. A., Cahalan, S. M., Qiu, Z., Hong, G., Crowley, C. S., Whitwam, T., Lee, W. H., Ward, A. B., et al. Structure of the human volume regulated anion channel eLife 2018 7 DOI:10.7554/eLife.38461 PMID:30095067 PMCID:PMC6086657
- Saotome, K., Murthy, S. E., Kefauver, J. M., Whitwam, T., Patapoutian, A., Ward, A. B. Structure of the mechanically activated ion channel Piezo1 Nature 2018 554:481-486 DOI:10.1038/nature25453 PMID:29261642
- Saphire, E. O., Schendel, S. L., Fusco, M. L., Gangavarapu, K., Gunn, B. M., Wec, A. Z., Halfmann, P. J., Brannan, J. M., Herbert, A. S., Qiu, X. G., Wagh, K., He, S., et al. Systematic analysis of monoclonal antibodies against Ebola virus GP defines features that contribute to protection Cell 2018 174:938-952 DOI:10.1016/j.cell.2018.07.033 PMID:30096313 PMCID:PMC6102396
- Dey, A. K., Cupo, A., Ozorowski, G., Sharma, V. K., Behrens, A. J., Go, E. P., Ketas, T. J., Yasmeen, A., Klasse, P. J., Sayeed, E., Desaire, H., Crispin, M., et al. cGMP production and analysis of BG505 SOSIP.664, an extensively glycosylated, trimeric HIV-1 envelope glycoprotein vaccine candidate Biotechnology and Bioengineering 2018 115:885-899 DOI:10.1002/bit.26498 PMID:29150937 PMCID:PMC5852640
- Lee, J. H., Andrabi, R., Su, C. Y., Yasmeen, A., Julien, J. P., Kong, L., Wu, N. C., McBride, R., Sok, D., Pauthner, M., Cottrell, C. A., Nieusma, T., et al. A broadly neutralizing antibody targets the dynamic HIV envelope trimer apex via a long, rigidified, and anionic β-hairpin structure Immunity 2017 46:690-702 DOI:10.1016/j.immuni.2017.03.017 PMID:28423342 PMCID:PMC5400778
- Wec, A. Z., Herbert, A. S., Murin, C. D., Nyakatura, E. K., Abelson, D. M., Fels, J. M., He, S., James, R. M., de La Vega, M. A., Zhu, W., Bakken, R. R., Goodwin, E., et al. Antibodies from a human survivor define sites of vulnerability for broad protection against Ebolaviruses Cell 2017 169:878-890 DOI:10.1016/j.cell.2017.04.037 PMID:28525755
- Kumar, R., Ozorowski, G., Kumar, V., Holden, L. G., Shrivastava, T., Patil, S., Deshpande, S., Ward, A. B., Bhattacharya, J. Characterization of a stable HIV-1B/C recombinant, soluble, and trimeric envelope glycoprotein (Env) highly resistant to CD4-induced conformational changes Journal of Biological Chemistry 2017 292:15849-15858 DOI:10.1074/jbc.M117.803056
- Strauch, E. M., Bernard, S. M., La, D., Bohn, A. J., Lee, P. S., Anderson, C. E., Nieusma, T., Holstein, C. A., Garcia, N. K., Hooper, K. A., Ravichandran, R., Nelson, J. W., et al. Computational design of trimeric influenza-neutralizing proteins targeting the hemagglutinin receptor binding site Nature Biotechnology 2017 35:667-671 DOI:10.1038/nbt.3907 PMID:28604661 PMCID:PMC5512607
- Howell, K. A., Brannan, J. M., Bryan, C., McNeal, A., Davidson, E., Turner, H. L., Vu, H., Shulenin, S., He, S., Kuehne, A., Herbert, A. S., Qiu, X., et al. Cooperativity enables non-neutralizing antibodies to neutralize Ebolavirus Cell Reports 2017 19:413-424 DOI:10.1016/j.celrep.2017.03.049 PMID:28402862
- Medina-Ramirez, M., Garces, F., Escolano, A., Skog, P., de Taeye, S. W., Del Moral-Sanchez, I., McGuire, A. T., Yasmeen, A., Behrens, A. J., Ozorowski, G., van den Kerkhof, Tlgm, Freund, N. T., et al. Design and crystal structure of a native-like HIV-1 envelope trimer that engages multiple broadly neutralizing antibody precursors in vivo Journal of Experimental Medicine 2017 214:2573-2590 DOI:10.1084/jem.20161160 PMID:28847869 PMCID:PMC5584115
- Morris, C. D., Azadnia, P., de Val, N., Vora, N., Honda, A., Giang, E., Saye-Francisco, K., Cheng, Y. S., Lin, X., Mann, C. J., Tang, J., Sok, D., et al. Differential antibody responses to conserved HIV-1 neutralizing epitopes in the context of multivalent scaffolds and native-like gp140 trimers mBio 2017 8:e00036-17 DOI:10.1128/mBio.00036-17 PMID:28246356 PMCID:PMC5347340
- Berndsen, Z., Bowman, C., Jang, H., Ward, A. B. EMHP: an accurate automated hole masking algorithm for single-particle cryo-EM image processing Bioinformatics 2017 33:3824-3826 DOI:10.1093/bioinformatics/btx500 PMID:28961740
- Voss, J. E., Andrabi, R., McCoy, L. E., de Val, N., Fuller, R. P., Messmer, T., Su, C. Y., Sok, D., Khan, S. N., Garces, F., Pritchard, L. K., Wyatt, R. T., et al. Elicitation of neutralizing antibodies targeting the V2 apex of the HIV Envelope trimer in a wild-type animal model Cell Reports 2017 21:222-235 DOI:10.1016/j.celrep.2017.09.024 PMID:28978475 PMCID:PMC5640805
- Pauthner, M., Havenar-Daughton, C., Sok, D., Nkolola, J. P., Bastidas, R., Boopathy, A. V., Carnathan, D. G., Chandrashekar, A., Cirelli, K. M., Cottrell, C. A., Eroshkin, A. M., Guenaga, J., et al. Elicitation of robust tier 2 neutralizing antibody responses in nonhuman primates by HIV envelope trimer immunization using optimized approaches Immunity 2017 46:1073-1088 DOI:10.1016/j.immuni.2017.05.007 PMID:28636956 PMCID:PMC5483234
- Guenaga, J., Garces, F., de Val, N., Stanfield, R. L., Dubrovskaya, V., Higgins, B., Carrette, B., Ward, A. B., Wilson, I. A., Wyatt, R. T. Glycine substitution at helix-to-coil transitions facilitates the structural determination of a stabilized subtype C HIV envelope glycoprotein Immunity 2017 46:792-+ DOI:10.1016/j.immuni.2017.04.014 PMID:28514686 PMCID:PMC5439057
- Landais, E., Murrell, B., Briney, B., Murrell, S., Rantalainen, K., Berndsen, Z. T., Ramos, A., Wickramasinghe, L., Smith, M. L., Eren, K., de Val, N., Wu, M., et al. HIV envelope glycoform heterogeneity and localized diversity govern the initiation and maturation of a V2 apex broadly neutralizing antibody lineage Immunity 2017 47:990-1003 DOI:10.1016/j.immuni.2017.11.002 PMID:29166592 PMCID:PMC5736302
- Wang, Y., O'Dell, S., Turner, H. L., Chiang, C. I., Lei, L., Guenaga, J., Wilson, R., Martinez-Murillo, P., Doria-Rose, N., Ward, A. B., Mascola, J. R., Wyatt, R. T., et al. HIV-1 cross-reactive primary virus neutralizing antibody response elicited by immunization in nonhuman primates Journal of Virology 2017 91:e00910-17 DOI:10.1128/jvi.00910-17 PMID:28835491 PMCID:PMC5640868
- He, L., Lin, X., de Val, N., Saye-Francisco, K. L., Mann, C. J., Augst, R., Morris, C. D., Azadnia, P., Zhou, B., Sok, D., Ozorowski, G., Ward, A. B., et al. Hidden lineage complexity of glycan-dependent HIV-1 broadly neutralizing antibodies uncovered by digital panning and native-like gp140 trimer Frontiers in Immunology 2017 8 DOI:10.3389/fimmu.2017.01025
- Sullivan, J. T., Sulli, C., Nilo, A., Yasmeen, A., Ozorowski, G., Sanders, R. W., Ward, A. B., Klasse, P. J., Moore, J. P., Doranz, B. J. High-throughput protein engineering improves the antigenicity and stability of soluble HIV-1 envelope glycoprotein SOSIP trimers Journal of Virology 2017 91:e00862-17 DOI:10.1128/jvi.00862-17 PMID:28878072 PMCID:PMC5660478
- Zhao, X., Howell, K. A., He, S., Brannan, J. M., Wec, A. Z., Davidson, E., Turner, H. L., Chiang, C. I., Lei, L., Fels, J. M., Vu, H., Shulenin, S., et al. Immunization-elicited broadly protective antibody reveals Ebolavirus fusion loop as a site of vulnerability Cell 2017 169:891-+ DOI:10.1016/j.cell.2017.04.038 PMID:28525756
- Pallesen, J., Wang, N., Corbett, K. S., Wrapp, D., Kirchdoerfer, R. N., Turner, H. L., Cottrell, C. A., Becker, M. M., Wang, L., Shi, W., Kong, W. P., Andres, E. L., et al. Immunogenicity and structures of a rationally designed prefusion MERS-CoV spike antigen Proceedings of the National Academy of Sciences of the United States of America 2017 114:E7348-E7357 DOI:10.1073/pnas.1707304114 PMID:28807998
- Ringe, R. P., Ozorowski, G., Yasmeen, A., Cupo, A., Portillo, V. M., Pugach, P., Golabek, M., Rantalainen, K., Holden, L. G., Cottrell, C. A., Wilson, I. A., Sanders, R. W., et al. Improving the expression and purification of soluble, recombinant native-like HIV-1 envelope glycoprotein trimers by targeted sequence changes Journal of Virology 2017 91:e00264-17 DOI:10.1128/jvi.00264-17 PMID:28381572
- Torrents de la Pena, A., Julien, J. P., de Taeye, S. W., Garces, F., Guttman, M., Ozorowski, G., Pritchard, L. K., Behrens, A. J., Go, E. P., Burger, J. A., Schermer, E. E., Sliepen, K., et al. Improving the immunogenicity of native-like HIV-1 envelope trimers by hyperstabilization Cell Reports 2017 20:1805-1817 DOI:10.1016/j.celrep.2017.07.077 PMID:28834745
- Wu, N. C., Grande, G., Turner, H. L., Ward, A. B., Xie, J., Lerner, R. A., Wilson, I. A. In vitro evolution of an influenza broadly neutralizing antibody is modulated by hemagglutinin receptor specificity Nature Communications 2017 8 DOI:10.1038/ncomms15371 PMID:28504265 PMCID:PMC5440694
- Ozorowski, G., Pallesen, J., de Val, N., Lyumkis, D., Cottrell, C. A., Torres, J. L., Copps, J., Stanfield, R. L., Cupo, A., Pugach, P., Moore, J. P., Wilson, I. A., et al. Open and closed structures reveal allostery and pliability in the HIV-1 envelope spike Nature 2017 547:360-363 DOI:10.1038/nature23010 PMID:28700571 PMCID:PMC5538736
- Sok, D., Le, K. M., Vadnais, M. L., Saye-Francisco, K. L., Jardine, J. G., Torres, J. L., Berndsen, Z. T., Kong, L., Tanfield, R. S., Ruiz, J., Ramos, A., Liang, C. H., et al. Rapid elicitation of broadly neutralizing antibodies to HIV by immunization in cows Nature 2017 548:108-111 DOI:10.1038/nature23301 PMID:28726771
- Ringe, R. P., Ozorowski, G., Rantalainen, K., Struwe, W. B., Matthews, K., Torres, J. L., Yasmeen, A., Cottrell, C. A., Ketas, T. J., LaBranche, C. C., Montefiori, D. C., Cupo, A., et al. Reducing V3 antigenicity and immunogenicity on soluble, native-like HIV-1 Env SOSIP trimers Journal of Virology 2017 91:e00677-17 DOI:10.1128/jvi.00677-17 PMID:28539451 PMCID:PMC5512241
- Koch, K., Kalusche, S., Torres, J. L., Stanfield, R. L., Danquah, W., Khazanehdari, K., von Briesen, H., Geertsma, E. R., Wilson, I. A., Wernery, U., Koch-Nolte, F., Ward, A. B., et al. Selection of nanobodies with broad neutralizing potential against primary HIV-1 strains using soluble subtype C gp140 envelope trimers Scientific Reports 2017 7 DOI:10.1038/s41598-017-08273-7 PMID:28827559 PMCID:PMC5566552
- Ahmed, S., Shrivastava, T., Kumar, N., Ozorowski, G., Ward, A. B., Chakrabarti, B. K. Stabilization of a soluble, native-like trimeric form of an efficiently cleaved Indian HIV-1 clade C envelope glycoprotein Journal of Biological Chemistry 2017 292:8236-8243 DOI:10.1074/jbc.M117.776419 PMID:28283570 PMCID:PMC5437231
- Oyen, D., Torres, J. L., Wille-Reece, U., Ockenhouse, C. F., Emerling, D., Glanville, J., Volkmuth, W., Flores-Garcia, Y., Zavala, F., Ward, A. B., King, C. R., Wilson, I. A. Structural basis for antibody recognition of the NANP repeats in Plasmodium falciparum circumsporozoite protein Proceedings of the National Academy of Sciences of the United States of America 2017 114:E10438-E10445 DOI:10.1073/pnas.1715812114 PMID:29138320 PMCID:PMC5715787
- Wibmer, C. K., Gorman, J., Ozorowski, G., Bhiman, J. N., Sheward, D. J., Elliott, D. H., Rouelle, J., Smira, A., Joyce, M. G., Ndabambi, N., Druz, A., Asokan, M., et al. Structure and recognition of a novel HIV-1 gp120-gp41 interface antibody that caused MPER exposure through viral escape PLoS Pathogens 2017 13 DOI:10.1371/journal.ppat.1006074 PMID:28076415 PMCID:PMC5226681
- Kulp, D. W., Steichen, J. M., Pauthner, M., Hu, X., Schiffner, T., Liguori, A., Cottrell, C. A., Havenar-Daughton, C., Ozorowski, G., Georgeson, E., Kalyuzhniy, O., Willis, J. R., et al. Structure-based design of native-like HIV-1 envelope trimers to silence non-neutralizing epitopes and eliminate CD4 binding Nature Communications 2017 8 DOI:10.1038/s41467-017-01549-6 PMID:29162799 PMCID:PMC5698488
- Dubrovskaya, V., Guenaga, J., de Val, N., Wilson, R., Feng, Y., Movsesyan, A., Hedestam, G. B. K., Ward, A. B., Wyatt, R. T. Targeted N-glycan deletion at the receptor-binding site retains HIV Env NFL trimer integrity and accelerates the elicited antibody response PLoS Pathogens 2017 13 DOI:10.1371/journal.ppat.1006614 PMID:28902916
- Cottrell, C. A., Ward, A. B. The 2017 Keystone Symposium on HIV vaccines Human Vaccines & Immunotherapeutics 2017 13:2348-2351 DOI:10.1080/21645515.2017.1367882 PMID:28786733 PMCID:PMC5647986
- Ward, A. B., Wilson, I. A. The HIV-1 envelope glycoprotein structure: nailing down a moving target Immunological Reviews 2017 275:21-32 DOI:10.1111/imr.12507 PMID:28133813 PMCID:PMC5300090
- Hopper, J. T. S., Ambrose, S., Grant, O. C., Krumm, S. A., Allison, T. M., Degiacomi, M. T., Tully, M. D., Pritchard, L. K., Ozorowski, G., Ward, A. B., Crispin, M., Doores, K. J., et al. The tetrameric plant lectin BanLec neutralizes HIV through bidentate binding to specific viral glycans Structure 2017 25:773-782 DOI:10.1016/j.str.2017.03.015 PMID:28434916
- Nogal, B., Bowman, C. A., Ward, A. B. Time-course, negative-stain electron microscopy-based analysis for investigating protein-protein interactions at the single-molecule level Journal of Biological Chemistry 2017 292:19400-19410 DOI:10.1074/jbc.M117.808352 PMID:28972148 PMCID:PMC5702678
- Cale, E. M., Gorman, J., Radakovich, N. A., Crooks, E. T., Osawa, K., Tong, T., Li, J. Q., Nagarajan, R., Ozorowski, G., Ambrozak, D. R., Asokan, M., Bailer, R. T., et al. Virus-like particles identify an HIV V1V2 apex-binding neutralizing antibody that lacks a protruding loop Immunity 2017 46:777-791 DOI:10.1016/j.immuni.2017.04.011 PMID:28514685
- Koday, M. T., Nelson, J., Chevalier, A., Koday, M., Kalinoski, H., Stewart, L., Carter, L., Nieusma, T., Lee, P. S., Ward, A. B., Wilson, I. A., Dagley, A., et al. A Computationally Designed Hemagglutinin Stem-Binding Protein Provides In Vivo Protection from Influenza Independent of a Host Immune Response PLoS Pathogens 2016 12 DOI:10.1371/journal.ppat.1005409 PMID:26845438 PMCID:PMC4742065
- Sok, D., Pauthner, M., Briney, B., Lee, J. H., Saye-Francisco, K. L., Hsueh, J., Ramos, A., Le, K. M., Jones, M., Jardine, J. G., Bastidas, R., Sarkar, A., et al. A prominent site of antibody vulnerability on HIV envelope incorporates a motif associated with CCR5 binding and its camouflaging glycans Immunity 2016 45:31-45 DOI:10.1016/j.immuni.2016.06.026 PMID:27438765 PMCID:PMC4990068
- van Gils, M. J., van den Kerkhof, T. L., Ozorowski, G., Cottrell, C. A., Sok, D., Pauthner, M., Pallesen, J., de Val, N., Yasmeen, A., de Taeye, S. W., Schorcht, A., Gumbs, S., et al. An HIV-1 antibody from an elite neutralizer implicates the fusion peptide as a site of vulnerability Nature Microbiology 2016 2 DOI:10.1038/nmicrobiol.2016.199 PMID:27841852 PMCID:PMC5372380
- Howell, K. A., Qiu, X., Brannan, J. M., Bryan, C., Davidson, E., Holtsberg, F. W., Wec, A. Z., Shulenin, S., Biggins, J. E., Douglas, R., Enterlein, S. G., Turner, H. L., et al. Antibody treatment of Ebola and Sudan virus infection via a uniquely exposed epitope within the glycoprotein receptor-binding site Cell Reports 2016 15:1514-1526 DOI:10.1016/j.celrep.2016.04.026 PMID:27160900 PMCID:PMC4871745
- Behrens, A. J., Vasiljevic, S., Pritchard, L. K., Harvey, D. J., Andev, R. S., Krumm, S. A., Struwe, W. B., Cupo, A., Kumar, A., Zitzmann, N., Seabright, G. E., Kramer, H. B., et al. Composition and antigenic effects of individual glycan sites of a trimeric HIV-1 envelope glycoprotein Cell Reports 2016 14:2695-2706 DOI:10.1016/j.celrep.2016.02.058 PMID:26972002 PMCID:PMC4805854
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- Julien, J. P., Sok, D., Khayat, R., Lee, J. H., Doores, K. J., Walker, L. M., Ramos, A., Diwanji, D. C., Pejchal, R., Cupo, A., Katpally, U., Depetris, R. S., et al. Broadly neutralizing antibody PGT121 allosterically modulates CD4 binding via recognition of the HIV-1 gp120 V3 base and multiple surrounding glycans PLoS Pathogens 2013 9:e1003342 DOI:10.1371/journal.ppat.1003342 PMID:23658524 PMCID:PMC3642082
- Ringe, R. P., Sanders, R. W., Yasmeen, A., Kim, H. J., Lee, J. H., Cupo, A., Korzun, J., Derking, R., van Montfort, T., Julien, J. P., Wilson, I. A., Klasse, P. J., et al. Cleavage strongly influences whether soluble HIV-1 envelope glycoprotein trimers adopt a native-like conformation Proceedings of the National Academy of Sciences of the United States of America 2013 110:18256-18261 DOI:10.1073/pnas.1314351110 PMID:24145402 PMCID:PMC3831437
- Lyumkis, D., Julien, J. P., de Val, N., Cupo, A., Potter, C. S., Klasse, P. J., Burton, D. R., Sanders, R. W., Moore, J. P., Carragher, B., Wilson, I. A., Ward, A. B. Cryo-EM structure of a fully glycosylated soluble cleaved HIV-1 envelope trimer Science 2013 342:1484-1490 DOI:10.1126/science.1245627 PMID:24179160 PMCID:PMC3954647
- Julien, J. P., Cupo, A., Sok, D., Stanfield, R. L., Lyumkis, D., Deller, M. C., Klasse, P. J., Burton, D. R., Sanders, R. W., Moore, J. P., Ward, A. B., Wilson, I. A. Crystal structure of a soluble cleaved HIV-1 envelope trimer Science 2013 342:1477-1483 DOI:10.1126/science.1245625 PMID:24179159 PMCID:PMC3886632
- Kong, L., Giang, E., Nieusma, T., Kadam, R. U., Cogburn, K. E., Hua, Y., Dai, X., Stanfield, R. L., Burton, D. R., Ward, A. B., Wilson, I. A., Law, M. Hepatitis C virus E2 envelope glycoprotein core structure Science 2013 342:1090-1094 DOI:10.1126/science.1243876 PMID:24288331 PMCID:PMC3954638
- Thornburg, N. J., Nannemann, D. P., Blum, D. L., Belser, J. A., Tumpey, T. M., Deshpande, S., Fritz, G. A., Sapparapu, G., Krause, J. C., Lee, J. H., Ward, A. B., Lee, D. E., et al. Human antibodies that neutralize respiratory droplet transmissible H5N1 influenza viruses Journal of Clinical Investigation 2013 123:4405-4409 DOI:10.1172/jci69377 PMID:23999429 PMCID:PMC3784541
- Klasse, P. J., Depetris, R. S., Pejchal, R., Julien, J. P., Khayat, R., Lee, J. H., Marozsan, A. J., Cupo, A., Cocco, N., Korzun, J., Yasmeen, A., Ward, A. B., et al. Influences on Trimerization and Aggregation of Soluble, Cleaved HIV-1 SOSIP Envelope Glycoprotein Journal of Virology 2013 87:9873-9885 DOI:10.1128/jvi.01226-13 PMID:23824824 PMCID:PMC3754145
- Jardine, J., Julien, J. P., Menis, S., Ota, T., Kalyuzhniy, O., McGuire, A., Sok, D., Huang, P. S., MacPherson, S., Jones, M., Nieusma, T., Mathison, J., et al. Rational HIV immunogen design to target specific germline B cell receptors Science 2013 340:711-716 DOI:10.1126/science.1234150 PMID:23539181 PMCID:PMC3689846
- Lee, S. C., Bennett, B. C., Hong, W. X., Fu, Y., Baker, K. A., Marcoux, J., Robinson, C. V., Ward, A. B., Halpert, J. R., Stevens, R. C., Stout, C. D., Yeager, M. J., et al. Steroid-based facial amphiphiles for stabilization and crystallization of membrane proteins Proceedings of the National Academy of Sciences of the United States of America 2013 110:E1203-11 DOI:10.1073/pnas.1221442110 PMID:23479627 PMCID:PMC3612649
- Wu, Y., West, A. P., Kim, H. J., Thornton, M. E., Ward, A. B., Bjorkman, P. J. Structural basis for enhanced HIV-1 neutralization by a dimeric immunoglobulin G form of the glycan-recognizing antibody 2G12 Cell Reports 2013 5:1443-1455 DOI:10.1016/j.celrep.2013.11.015 PMID:24316082 PMCID:PMC3919625
- Khayat, R., Lee, J. H., Julien, J. P., Cupo, A., Klasse, P. J., Sanders, R. W., Moore, J. P., Wilson, I. A., Ward, A. B. Structural characterization of cleaved, soluble HIV-1 envelope glycoprotein trimers Journal of Virology 2013 87:9865-9872 DOI:10.1128/jvi.01222-13 PMID:23824817 PMCID:PMC3754114
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- Kong, L., Lee, J. H., Doores, K. J., Murin, C. D., Julien, J. P., McBride, R., Liu, Y., Marozsan, A., Cupo, A., Klasse, P. J., Hoffenberg, S., Caulfield, M., et al. Supersite of immune vulnerability on the glycosylated face of HIV-1 envelope glycoprotein gp120 Nature Structural & Molecular Biology 2013 20:796-803 DOI:10.1038/nsmb.2594 PMID:23708606 PMCID:PMC3823233
- Burton, D. R., Ahmed, R., Barouch, D. H., Butera, S. T., Crotty, S., Godzik, A., Kaufmann, D. E., McElrath, M. J., Nussenzweig, M. C., Pulendran, B., Scanlan, C. N., Schief, W. R., et al. A blueprint for HIV vaccine discovery Cell Host & Microbe 2012 12:396-407 DOI:10.1016/j.chom.2012.09.008 PMID:23084910 PMCID:PMC3513329
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Ward, A. B., Guvench, O., Hills, R. D.
Coarse grain lipid-protein molecular interactions and diffusion with MsbA flippase
Proteins-Structure Function and Bioinformatics
2012
80:2178-2190
DOI:10.1002/prot.24108
PMID:22552999
- Ekiert, D. C., Kashyap, A. K., Steel, J., Rubrum, A., Bhabha, G., Khayat, R., Lee, J. H., Dillon, M. A., O'Neil, R. E., Faynboym, A. M., Horowitz, M., Horowitz, L., et al. Cross-neutralization of influenza a viruses mediated by a single antibody loop Nature 2012 489:526-32 DOI:10.1038/nature11414 PMID:22982990 PMCID:PMC3538848
- Dreyfus, C., Laursen, N. S., Kwaks, T., Zuijdgeest, D., Khayat, R., Ekiert, D. C., Lee, J. H., Metlagel, Z., Bujny, M. V., Jongeneelen, M., van der Vlugt, R., Lamrani, M., et al. Highly conserved protective epitopes on influenza B viruses Science 2012 337:1343-1348 DOI:10.1126/science.1222908 PMID:22878502 PMCID:PMC3538841
- Depetris, R. S., Julien, J. P., Khayat, R., Lee, J. H., Pejchal, R., Katpally, U., Cocco, N., Kachare, M., Massi, E., David, K. B., Cupo, A., Marozsan, A. J., et al. Partial enzymatic deglycosylation preserves the structure of cleaved recombinant HIV-1 envelope glycoprotein trimers Journal of Biological Chemistry 2012 287:24239-24254 DOI:10.1074/jbc.M112.371898 PMID:22645128 PMCID:PMC3397850
- Bai, J. P., Swartz, D. J., Protasevich, II, Brouillette, C. G., Harrell, P. M., Hildebrandt, E., Gasser, B., Mattanovich, D., Ward, A., Chang, G., Urbatsch, I. L. A gene optimization strategy that enhances production of fully functional p-glycoprotein in pichia pastoris PLoS One 2011 6:e22577 DOI:10.1371/journal.pone.0022577 PMID:21826197 PMCID:PMC3149604
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Fisher, L. S., Ward, A., Milligan, R. A., Unwin, N., Potter, C. S., Carragher, B.
A helical processing pipeline for EM structure determination of membrane proteins
Methods
2011
55:350-362
DOI:10.1016/j.ymeth.2011.09.013
PMID:21964395
PMCID:PMC3262078
- Pejchal, R., Doores, K. J., Walker, L. M., Khayat, R., Huang, P. S., Wang, S. K., Stanfield, R. L., Julien, J. P., Ramos, A., Crispin, M., Depetris, R., Katpally, U., et al. A potent and broad neutralizing antibody recognizes and penetrates the HIV glycan shield Science 2011 334:1097-1103 DOI:10.1126/science.1213256 PMID:21998254 PMCID:PMC3280215
- McLellan, J. S., Pancera, M., Carrico, C., Gorman, J., Julien, J. P., Khayat, R., Louder, R., Pejchal, R., Sastry, M., Dai, K. F., O'Dell, S., Patel, N., et al. Structure of HIV-1 gp120 V1/V2 domain with broadly neutralizing antibody PG9 Nature 2011 480:336-343 DOI:10.1038/nature10696 PMID:22113616 PMCID:PMC3406929
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Perz-Edwards, R. J., Irving, T. C., Baumann, B. A. J., Gore, D., Hutchinson, D. C., Krzic, U., Porter, R. L., Ward, A. B., Reedy, M. K.
X-ray diffraction evidence for myosin-troponin connections and tropomyosin movement during stretch activation of insect flight muscle
Proceedings of the National Academy of Sciences of the United States of America
2011
108:120-125
DOI:10.1073/pnas.1014599107
PMID:21148419
PMCID:PMC3017141
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Glaves, J. P., Fisher, L., Ward, A., Young, H. S.
Helical crystallization of two example membrane proteins MsbA and the Ca(2+)-ATPase
Methods in Enzymology
2010
483:143-159
DOI:10.1016/s0076-6879(10)83007-1
PMID:20888473
- Gutmann, D. A. P., Ward, A., Urbatsch, I. L., Chang, G., van Veen, H. W. Understanding polyspecificity of multidrug ABC transporters: closing in on the gaps in ABCB1 Trends in Biochemical Sciences 2010 35:36-42 DOI:10.1016/j.tibs.2009.07.009 PMID:19819701 PMCID:PMC4608440
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Ward, A., Mulligan, S., Carragher, B., Chang, G., Milligan, R. A.
Nucleotide dependent packing differences in helical crystals of the ABC transporter MsbA
Journal of Structural Biology
2009
165:169-175
DOI:10.1016/j.jsb.2008.11.006
PMID:19114108
PMCID:PMC2703300
- Aller, S. G., Yu, J., Ward, A., Weng, Y., Chittaboina, S., Zhuo, R. P., Harrell, P. M., Trinh, Y. T., Zhang, Q. H., Urbatsch, I. L., Chang, G. Structure of P-glycoprotein reveals a molecular basis for poly-specific drug binding Science 2009 323:1718-1722 DOI:10.1126/science.1168750 PMID:19325113 PMCID:PMC2720052
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Bekyarova, T. I., Reedy, M. C., Baumann, B. A. J., Tregear, R. T., Ward, A., Krzic, U., Prince, K. M., Perz-Edwards, R. J., Reconditi, M., Gore, D., Irving, T. C., Reedy, M. K.
Reverse actin sliding triggers strong myosin binding that moves tropomyosin
Proceedings of the National Academy of Sciences of the United States of America
2008
105:10372-10377
DOI:10.1073/pnas.0709877105
PMID:18658238
PMCID:PMC2492518
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Littlefield, K. P., Ward, A. B., Chappie, J. S., Reedy, M. K., Bernstein, S. I., Milligan, R. A., Reedy, M. C.
Similarities and differences between frozen-hydrated, rigor acto-S1 complexes of insect flight and chicken skeletal muscles
Journal of Molecular Biology
2008
381:519-528
DOI:10.1016/j.jmb.2008.06.029
PMID:18588896
PMCID:PMC2567842
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Zhang, Q. H., Ma, X. Q., Ward, A., Hong, W. X., Jaakola, V. P., Stevens, R. C., Finn, M. G., Chang, G.
Designing facial amphiphiles for the stabilization of integral membrane proteins
Angewandte Chemie-International Edition
2007
46:7023-7025
DOI:10.1002/anie.200701556
PMID:17691085
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Ward, A., Reyes, C. L., Yu, J., Roth, C. B., Chang, G.
Flexibility in the ABC transporter MsbA: Alternating access with a twist
Proceedings of the National Academy of Sciences of the United States of America
2007
104:19005-19010
DOI:10.1073/pnas.0709388104
PMID:18024585
PMCID:PMC2141898
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Reyes, C. L., Ward, A., Yu, J., Chang, G.
The structures of MsbA: Insight into ABC transporter-mediated multidrug efflux
FEBS Letters
2006
580:1042-1048
DOI:10.1016/j.febslet.2005.11.033
PMID:16337944
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Ward, A., Moody, M. F., Sheehan, B., Milligan, R. A., Carragher, B.
Windex: A toolset for indexing helices
Journal of Structural Biology
2003
144:172-183
DOI:10.1016/j.jsb.2003.10.008
PMID:14643220
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