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Ward, Andrew
Faculty Member
Graduate Student
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Positions
- 2017 - Professor, Integrative Structural and Computational Biology (ISCB) , Scripps Research
- 2010 - Faculty Member, Skaggs Graduate School of Chemical and Biological Sciences , Scripps Research
- 2015 - 2017 Associate Professor, Integrative Structural and Computational Biology (ISCB) , Scripps Research
- 2013 - 2015 Assistant Professor, Integrative Structural and Computational Biology (ISCB) , Scripps Research
- 2010 - 2012 Assistant Professor, Molecular Biology , Scripps Research
- 2008 - 2010 Research Associate with Dr. Geoffrey Chang, Molecular Biology , Scripps Research
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Publications
recent publications
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academic article
- Bangaru, S., Lang, S., Schotsaert, M., Vanderven, H. A., Zhu, X., Kose, N., Bombardi, R., Finn, J. A., Kent, S. J., Gilchuk, P., Gilchuk, I., Turner, H. L., et al. A site of vulnerability on the influenza virus hemagglutinin head domain trimer interface Cell 2019 177:1136-1152 DOI:10.1016/j.cell.2019.04.011 PMID:31100268
- Murin, C. D., Wilson, I. A., Ward, A. B. Antibody responses to viral infections: a structural perspective across three different enveloped viruses Nature Microbiology 2019 4:734-747 DOI:10.1038/s41564-019-0392-y PMID:30886356
- Winarski, K. L., Tang, J., Klenow, L., Lee, J., Coyle, E. M., Manischewitz, J., Turner, H. L., Takeda, K., Ward, A. B., Golding, H., Khurana, S. Antibody-dependent enhancement of influenza disease promoted by increase in hemagglutinin stem flexibility and virus fusion kinetics Proceedings of the National Academy of Sciences of the United States of America 2019 116:15194-15199 DOI:10.1073/pnas.1821317116 PMID:31296560 PMCID:PMC6660725
- Kumar, S., Sarkar, A., Pugach, P., Sanders, R. W., Moore, J. P., Ward, A. B., Wilson, I. A. Capturing the inherent structural dynamics of the HIV-1 envelope glycoprotein fusion peptide Nature Communications 2019 10 DOI:10.1038/s41467-019-08738-5 PMID:30770829 PMCID:PMC6377653
- Ringe, R. P., Pugach, P., Cottrell, C. A., LaBranche, C. C., Seabright, G. E., Ketas, T. J., Ozorowski, G., Kumar, S., Schorcht, A., van Gils, M. J., Crispin, M., Montefiori, D. C., et al. Closing and opening holes in the glycan shield of HIV-1 envelope glycoprotein SOSIP trimers can redirect the neutralizing antibody response to the newly unmasked epitopes Journal of Virology 2019 93:e01656-18 DOI:10.1128/jvi.01656-18 PMID:30487280 PMCID:PMC6363999
- Yuan, M.*, Cottrell, C. A.*, Ozorowski, G., van Gils, M. J., Kumar, S., Wu, N. C., Sarkar, A., Torres, J. L., de Val, N., Copps, J., Moore, J. P., Sanders, R. W., et al. Conformational plasticity in the HIV-1 fusion peptide facilitates recognition by broadly neutralizing antibodies Cell Host & Microbe 2019 25:873-883 DOI:10.1016/j.chom.2019.04.011 PMID:31194940 (* = equal contribution)
- Kirchdoerfer, R. N., Saphire, E. O., Ward, A. B. Cryo-EM structure of the Ebola virus nucleoprotein-RNA complex Acta Crystallographica Section F-Structural Biology Communications 2019 75:340-347 DOI:10.1107/s2053230x19004424 PMID:31045563 PMCID:PMC6497097
- Whitaker, N., Hickey, J. M., Kaur, K., Xiong, J., Sawant, N., Cupo, A., Lee, W. H., Ozorowski, G., Medina-Ramirez, M., Ward, A. B., Sanders, R. W., Moore, J. P., et al. Developability assessment of physicochemical properties and stability profiles of HIV-1 BG505 SOSIP.664 and BG505 SOSIP.v4.1-GT1.1 gp140 envelope glycoprotein trimers as candidate vaccine antigens Journal of Pharmaceutical Sciences 2019 108:2264-2277 DOI:10.1016/j.xphs.2019.01.033 PMID:30776383 PMCID:PMC6595180
- von Bredow, B., Andrabi, R., Grunst, M., Grandea, A. G., Le, K., Song, G., Berndsen, Z. T., Porter, K., Pallesen, J., Ward, A. B., Burton, D. R., Evans, D. T. Differences in the binding affinity of an HIV-1 V2 apex-specific antibody for the SIVsmm/mac envelope glycoprotein uncouple antibody-dependent cellular cytotoxicity from neutralization Mbio 2019 10:e01255-19 DOI:10.1128/mBio.01255-19 PMID:31266872 PMCID:PMC6606807
- Brouwer, P. J. M., Antanasijevic, A., Berndsen, Z., Yasmeen, A., Fiala, B., Bijl, T. P., Bontjer, I., Bale, J. B., Sheffler, W., Allen, J. D., Schorcht, A., Burgers, J. A., et al. Enhancing and shaping the immunogenicity of native-like HIV-1 envelope trimers with a two-component protein nanoparticle Nature Communications 2019 10 DOI:10.1038/s41467-019-12080-1 PMID:31537780 PMCID:PMC6753213
- Meuser, M. E., Rashad, A. A., Ozorowski, G., Dick, A., Ward, A. B., Cocklin, S. Field-based affinity optimization of a novel azabicyclohexane scaffold HIV-1 entry inhibitor Molecules 2019 24 DOI:10.3390/molecules24081581 PMID:31013646 PMCID:PMC6514670
- Nemanichvili, N., Tomris, I., Turner, H. L., McBride, R., Grant, O. C., van der Woude, R., Aldosari, M. H., Pieters, R. J., Woods, R. J., Paulson, J. C., Boons, G. J., Ward, A. B., et al. Fluorescent trimeric hemagglutinins reveal multivalent receptor binding properties Journal of Molecular Biology 2019 431:842-856 DOI:10.1016/j.jmb.2018.12.014 PMID:30597163 PMCID:PMC6397626
- Ward, A. B. Playing Chess with HIV Immunity 2019 50:283-285 DOI:10.1016/j.immuni.2019.01.011 PMID:30784575
- Turner, H. L., Pallesen, J., Lang, S., Bangaru, S., Urata, S., Li, S., Cottrell, C. A., Bowman, C. A., Crowe, J. E., Wilson, I. A., Ward, A. B. Potent anti-influenza H7 human monoclonal antibody induces separation of hemagglutinin receptor-binding head domains PLoS Biology 2019 17 DOI:10.1371/journal.pbio.3000139 PMID:30716060 PMCID:PMC6375650
- Torrents de la Pena, A., Rantalainen, K., Cottrell, C. A., Allen, J. D., van Gils, M. J., Torres, J. L., Crispin, M., Sanders, R. W., Ward, A. B. Similarities and differences between native HIV-1 envelope glycoprotein trimers and stabilized soluble trimer mimetics PLoS Pathogens 2019 15 DOI:10.1371/journal.ppat.1007920 PMID:31306470 PMCID:PMC6658011
- Cirelli, K. M., Carnathan, D. G., Nogal, B., Martin, J. T., Rodriguez, O. L., Upadhyay, A. A., Enemuo, C. A., Gebru, E. H., Choe, Y., Viviano, F., Nakao, C., Pauthner, M. G., et al. Slow delivery immunization enhances HIV neutralizing antibody and germinal center responses via modulation of immunodominance Cell 2019 177:1153-1171 DOI:10.1016/j.cell.2019.04.012 PMID:31080066
- de Taeye, S. W., Go, E. P., Sliepen, K., de la Pena, A. T., Badal, K., Medina-Ramirez, M., Lee, W. H., Desaire, H., Wilson, I. A., Moore, J. P., Ward, A. B., Sanders, R. W. Stabilization of the V2 loop improves the presentation of V2 loop-associated broadly neutralizing antibody epitopes on HIV-1 envelope trimers Journal of Biological Chemistry 2019 294:5616-5631 DOI:10.1074/jbc.RA118.005396 PMID:30728245 PMCID:PMC6462529
- Wang, N., Rosen, O., Wang, L., Turner, H. L., Stevens, L. J., Corbett, K. S., Bowman, C. A., Pallesen, J., Shi, W., Zhang, Y., Leung, K., Kirchdoerfer, R. N., et al. Structural definition of a neutralization-sensitive epitope on the MERS-CoV S1-NTD Cell Reports 2019 28:3395-3405 DOI:10.1016/j.celrep.2019.08.052 PMID:31553909
- Sliepen, K., Han, B. W., Bontjer, I., Mooij, P., Garces, F., Behrens, A. J., Rantalainen, K., Kumar, S., Sarkar, A., Brouwer, P. J. M., Hua, Y., Tolazzi, M., et al. Structure and immunogenicity of a stabilized HIV-1 envelope trimer based on a group-M consensus sequence Nature Communications 2019 10 DOI:10.1038/s41467-019-10262-5 PMID:31142746 PMCID:PMC6541627
- Kirchdoerfer, R. N., Ward, A. B. Structure of the SARS-CoV nsp12 polymerase bound to nsp7 and nsp8 co-factors Nature Communications 2019 10 DOI:10.1038/s41467-019-10280-3 PMID:31138817 PMCID:PMC6538669
- Saotome, K., Teng, B., Tsui, C. C., Lee, W. H., Tu, Y. H., Kaplan, J. P., Sansom, M. S. P., Liman, E. R., Ward, A. B. Structures of the otopetrin proton channels Otop1 and Otop3 Nature Structural & Molecular Biology 2019 26:518-525 DOI:10.1038/s41594-019-0235-9 PMID:31160780 PMCID:PMC6564688
- Lei, L., Yang, Y. H. R., Tran, K., Wang, Y. M., Chiang, C. I., Ozorowski, G., Xiao, Y., Ward, A. B., Wyatt, R. T., Li, Y. The HIV-1 Envelope glycoprotein C3/V4 region defines a prevalent neutralization epitope following immunization Cell Reports 2019 27:586-598 DOI:10.1016/j.celrep.2019.03.039 PMID:30970260 PMCID:PMC6458978
- Andrabi, R., Pallesen, J., Allen, J. D., Song, G., Zhang, J., de Val, N., Gegg, G., Porter, K., Su, C., Pauthner, M., Newman, A., Bouton-Verville, H., et al. The chimpanzee SIV envelope trimer: structure and deployment as an HIV vaccine template Cell Reports 2019 27:2426-2441 DOI:10.1016/j.celrep.2019.04.082 PMID:31116986 PMCID:PMC6533203
- Pauthner, M. G., Nkolola, J. P., Havenar-Daughton, C., Murrell, B., Reiss, S. M., Bastidas, R., Prevost, J., Nedellec, R., von Bredow, B., Abbink, P., Cottrell, C. A., Kulp, D. W., et al. Vaccine-induced protection from homologous tier 2 SHIV challenge in nonhuman primates depends on serum-neutralizing antibody titers Immunity 2019 50:241-252 DOI:10.1016/j.immuni.2018.11.011 PMID:30552025 PMCID:PMC6335502
- Bangaru, S., Zhang, H., Gilchuk, I. M., Voss, T. G., Irving, R. P., Gilchuk, P., Matta, P., Zhu, X., Lang, S., Nieusma, T., Richt, J. A., Albrecht, R. A., et al. A multifunctional human monoclonal neutralizing antibody that targets a unique conserved epitope on influenza HA Nature Communications 2018 9 DOI:10.1038/s41467-018-04704-9 PMID:29991715 PMCID:PMC6039445
- Flyak, A. I., Kuzmina, N., Murin, C. D., Bryan, C., Davidson, E., Gilchuk, P., Gulka, C. P., Ilinykh, P. A., Shen, X. L., Huang, K., Ramanathan, P., Turner, H., et al. Broadly neutralizing antibodies from human survivors target a conserved site in the Ebola virus glycoprotein HR2-MPER region Nature Microbiology 2018 3:670-677 DOI:10.1038/s41564-018-0157-z PMID:29736037
- Bale, S., Martine, A., Wilson, R., Behrens, A. J., Le Fourn, V., de Val, N., Sharma, S. K., Tran, K., Torres, J. L., Girod, P. A., Ward, A. B., Crispin, M., et al. Cleavage -Independent HIV-1 Trimers From CHO Cell Lines Elicit Robust Autologous Tier 2 Neutralizing Antibodies Frontiers in Immunology 2018 9 DOI:10.3389/fimmu.2018.01116 PMID:29881382 PMCID:PMC5976746
- Rantalainen, K., Berndsen, Z. T., Murrell, S., Cao, L., Omorodion, O., Torres, J. L., Wu, M., Umotoy, J., Copps, J., Poignard, P., Landais, E., Paulson, J. C., et al. Co-evolution of HIV envelope and apex-targeting neutralizing antibody lineage provides benchmarks for vaccine design Cell Reports 2018 23:3249-3261 DOI:10.1016/j.celrep.2018.05.046 PMID:29898396 PMCID:PMC6019700
- Oyen, D., Torres, J. L., Cottrell, C. A., King, C. R., Wilson, I. A., Ward, A. B. Cryo-EM structure of P. falciparum circumsporozoite protein with a vaccine-elicited antibody is stabilized by somatically mutated inter-Fab contacts Science Advances 2018 4 DOI:10.1126/sciadv.aau8529 PMID:30324137 PMCID:PMC6179375
- Jojoa-Cruz, S., Saotome, K., Murthy, S. E., Tsui, C. C. A., Sansom, M. S. P., Patapoutian, A., Ward, A. B. Cryo-EM structure of the mechanically activated ion channel OSCA1.2 Elife 2018 7 DOI:10.7554/eLife.41845 PMID:30382939 PMCID:PMC6235563
- Wu, N. C., Ward, A. B. Deception through mimicry: a cellular antiviral strategy Cell 2018 175:1728-1729 DOI:10.1016/j.cell.2018.11.033 PMID:30550784
- Pascal, K. E., Dudgeon, D., Trefry, J. C., Anantpadma, M., Sakurai, Y., Murin, C. D., Turner, H. L., Fairhurst, J., Torres, M., Rafique, A., Yan, Y., Badithe, A., et al. Development of clinical-stage human monoclonal antibodies that treat advanced Ebola virus disease in nonhuman primates Journal of Infectious Diseases 2018 218:S612-S626 DOI:10.1093/infdis/jiy285 PMID:29860496 PMCID:PMC6249601
- Cao, L. W., Pauthner, M., Andrabi, R., Rantalainen, K., Berndsen, Z., Diedrich, J. K., Menis, S., Sok, D., Bastidas, R., Park, S. K. R., Delahunty, C. M., He, L., et al. Differential processing of HIV envelope glycans on the virus and soluble recombinant trimer Nature Communications 2018 9 DOI:10.1038/s41467-018-06121-4 PMID:30209313 PMCID:PMC6135743
- Ozorowski, G., Cupo, A., Golabek, M., LoPiccolo, M., Ketas, T. A., Cavallary, M., Cottrell, C. A., Klasse, P. J., Ward, A. B., Moore, J. P. Effects of adjuvants on HIV-1 envelope glycoprotein SOSIP trimers in vitro Journal of Virology 2018 92 DOI:10.1128/jvi.00381-18 PMID:29669838 PMCID:PMC6002727
- Bianchi, M., Turner, H. L., Nogal, B., Cottrell, C. A., Oyen, D., Pauthner, M., Bastidas, R., Nedellec, R., McCoy, L. E., Wilson, I. A., Burton, D. R., Ward, A. B., et al. Electron-microscopy-based epitope mapping defines specificities of polyclonal antibodies elicited during HIV-1 BG505 envelope trimer immunization Immunity 2018 49:288-300 DOI:10.1016/j.immuni.2018.07.009 PMID:30097292 PMCID:PMC6104742
- Shrivastava, T., Samal, S., Tyagi, A. K., Goswami, S., Kumar, N., Ozorowski, G., Ward, A. B., Chakrabarti, B. K. Envelope proteins of two HIV-1 clades induced different epitope-specific antibody response Vaccine 2018 36:1627-1636 DOI:10.1016/j.vaccine.2018.01.081 PMID:29429810
- Klasse, P. J., Ketas, T. J., Cottrell, C. A., Ozorowski, G., Debnath, G., Camara, D., Francomano, E., Pugach, P., Ringe, R. P., LaBranche, C. C., van Gils, M. J., Bricault, C. A., et al. Epitopes for neutralizing antibodies induced by HIV-1 envelope glycoprotein BG505 SOSIP trimers in rabbits and macaques PLoS Pathogens 2018 14 DOI:10.1371/journal.ppat.1006913 PMID:29474444 PMCID:PMC5841823
- Maurer, M. A., Meyer, L., Bianchi, M., Turner, H. L., Le, N. P. L., Steck, M., Wyrzucki, A., Orlowski, V., Ward, A. B., Crispin, M., Hangartner, L. Glycosylation of human IgA directly inhibits influenza A and other sialic-acid-binding viruses Cell Reports 2018 23:90-99 DOI:10.1016/j.celrep.2018.03.027 PMID:29617676
- He, L., Kumar, S., Allen, J. D., Huang, D. L., Lin, X., Mann, C. J., Saye-Francisco, K. L., Copps, J., Sarkar, A., Blizard, G. S., Ozorowski, G., Sok, D., et al. HIV-1 vaccine design through minimizing envelope metastability Science Advances 2018 4 DOI:10.1126/sciadv.aau6769 PMID:30474059 PMCID:PMC6248932
- Behrens, A. J., Kumar, A., Medina-Ramirez, M., Cupo, A., Marshall, K., Portillo, V. M. C., Harvey, D. J., Ozorowski, G., Zitzmann, N., Wilson, I. A., Ward, A. B., Struwe, W. B., et al. Integrity of glycosylation processing of a glycan-depleted trimeric HIV-1 immunogen targeting key B-cell lineages Journal of Proteome Research 2018 17:987-999 DOI:10.1021/acs.jproteome.7b00639 PMID:29420040 PMCID:PMC5846105
- Gilchuk, P., Kuzmina, N., Ilinykh, P. A., Huang, K., Gunn, B. M., Bryan, A., Davidson, E., Doranz, B. J., Turner, H. L., Fusco, M. L., Bramble, M. S., Hoff, N. A., et al. Multifunctional pan-ebolavirus antibody recognizes a site of broad vulnerability on the ebolavirus glycoprotein Immunity 2018 49:363-374 DOI:10.1016/j.immuni.2018.06.018 PMID:30029854 PMCID:PMC6104738
- Murthy, S. E., Dubin, A. E., Whitwam, T., Jojoa-Cruz, S., Cahalan, S. M., Mousavi, S. A. R., Ward, A. B., Patapoutian, A. OSCA/TMEM63 are an evolutionarily conserved family of mechanically activated ion channels Elife 2018 7 DOI:10.7554/eLife.41844 PMID:30382938 PMCID:PMC6235560
- Aldon, Y., McKay, P. F., Allen, J., Ozorowski, G., Levai, R. F., Tolazzi, M., Rogers, P., He, L., de Val, N., Fabian, K., Scarlatti, G., Zhu, J., et al. Rational design of DNA-expressed stabilized native-like HIV-1 envelope trimers Cell Reports 2018 24:3324-3338 DOI:10.1016/j.celrep.2018.08.051 PMID:30232012 PMCID:PMC6167709
- Steinhardt, J. J., Guenaga, J., Turner, H. L., McKee, K., Louder, M. K., O'Dell, S., Chiang, C. I., Lei, L., Galkin, A., Andrianov, A. K., Doria-Rose, N. A., Bailer, R. T., et al. Rational design of a trispecific antibody targeting the HIV-1 Env with elevated anti-viral activity Nature Communications 2018 9 DOI:10.1038/s41467-018-03335-4 PMID:29491415 PMCID:PMC5830440
- de Taeye, S. W., de la Pena, A. T., Vecchione, A., Scutigliani, E., Sliepen, K., Burger, J. A., van der Woude, P., Schorcht, A., Schermer, E. E., van Gils, M. J., LaBranche, C. C., Montefiori, D. C., et al. Stabilization of the gp120 V3 loop through hydrophobic interactions reduces the immunodominant V3-directed non-neutralizing response to HIV-1 envelope trimers Journal of Biological Chemistry 2018 293:1688-1701 DOI:10.1074/jbc.RA117.000709 PMID:29222332 PMCID:PMC5798299
- Kirchdoerfer, R. N., Wang, N., Pallesen, J., Wrapp, D., Turner, H. L., Cottrell, C. A., Corbett, K. S., Graham, B. S., McLellan, J. S., Ward, A. B. Stabilized coronavirus spikes are resistant to conformational changes induced by receptor recognition or proteolysis Scientific Reports 2018 8 DOI:10.1038/s41598-018-34171-7 PMID:30356097 PMCID:PMC6200764
- Schiffner, T., Pallesen, J., Russell, R. A., Dodd, J., de Val, N., LaBranche, C. C., Montefiori, D., Tomaras, G. D., Shen, X., Harris, S. L., Moghaddam, A. E., Kalyuzhniy, O., et al. Structural and immunologic correlates of chemically stabilized HIV-1 envelope glycoproteins PLoS Pathogens 2018 14 DOI:10.1371/journal.ppat.1006986 PMID:29746590 PMCID:PMC5944921
- Murin, C. D., Bruhn, J. F., Bornholdt, Z. A., Copps, J., Stanfield, R., Ward, A. B. Structural basis of pan-Ebolavirus neutralization by an antibody targeting the glycoprotein fusion loop Cell Reports 2018 24:2723-2732.e4 DOI:10.1016/j.celrep.2018.08.009 PMID:30184505
- Crispin, M., Ward, A. B., Wilson, I. A. Structure and immune recognition of the HIV glycan shield Annual Review of Biophysics 2018 47:499-523 DOI:10.1146/annurev-biophys-060414-034156 PMID:29595997
- Sarkar, A., Bale, S., Behrens, A. J., Kumar, S., Sharma, S. K., de Val, N., Pallesen, J., Irimia, A., Diwanji, D. C., Stanfield, R. L., Ward, A. B., Crispin, M., et al. Structure of a cleavage-independent HIV Env recapitulates the glycoprotein architecture of the native cleaved trimer Nature Communications 2018 9 DOI:10.1038/s41467-018-04272-y PMID:29769533 PMCID:PMC5955915
- Kefauver, J. M., Saotome, K., Dubin, A. E., Pallesen, J., Cottrell, C. A., Cahalan, S. M., Qiu, Z., Hong, G., Crowley, C. S., Whitwam, T., Lee, W. H., Ward, A. B., et al. Structure of the human volume regulated anion channel Elife 2018 7 DOI:10.7554/eLife.38461 PMID:30095067 PMCID:PMC6086657
- Saotome, K., Murthy, S. E., Kefauver, J. M., Whitwam, T., Patapoutian, A., Ward, A. B. Structure of the mechanically activated ion channel Piezo1 Nature 2018 554:481-486 DOI:10.1038/nature25453 PMID:29261642
- Saphire, E. O., Schendel, S. L., Fusco, M. L., Gangavarapu, K., Gunn, B. M., Wec, A. Z., Halfmann, P. J., Brannan, J. M., Herbert, A. S., Qiu, X. G., Wagh, K., He, S., et al. Systematic analysis of monoclonal antibodies against Ebola virus GP defines features that contribute to protection Cell 2018 174:938-952 DOI:10.1016/j.cell.2018.07.033 PMID:30096313 PMCID:PMC6102396
- Laursen, N. S., Friesen, R. H. E., Zhu, X., Jongeneelen, M., Blokland, S., Vermond, J., van Eijgen, A., Tang, C., van Diepen, H., Obmolova, G., Kolfschoten, M. V., Zuijdgeest, D., et al. Universal protection against influenza infection by a multidomain antibody to influenza hemagglutinin Science 2018 362:598-602 DOI:10.1126/science.aaq0620 PMID:30385580 PMCID:PMC6241527
- Dey, A. K., Cupo, A., Ozorowski, G., Sharma, V. K., Behrens, A. J., Go, E. P., Ketas, T. J., Yasmeen, A., Klasse, P. J., Sayeed, E., Desaire, H., Crispin, M., et al. cGMP production and analysis of BG505 SOSIP.664, an extensively glycosylated, trimeric HIV-1 envelope glycoprotein vaccine candidate Biotechnology and Bioengineering 2018 115:885-899 DOI:10.1002/bit.26498 PMID:29150937 PMCID:PMC5852640
- Lee, J. H., Andrabi, R., Su, C. Y., Yasmeen, A., Julien, J. P., Kong, L., Wu, N. C., McBride, R., Sok, D., Pauthner, M., Cottrell, C. A., Nieusma, T., et al. A broadly neutralizing antibody targets the dynamic HIV envelope trimer apex via a long, rigidified, and anionic β-hairpin structure Immunity 2017 46:690-702 DOI:10.1016/j.immuni.2017.03.017 PMID:28423342 PMCID:PMC5400778
- Wec, A. Z., Herbert, A. S., Murin, C. D., Nyakatura, E. K., Abelson, D. M., Fels, J. M., He, S., James, R. M., de La Vega, M. A., Zhu, W., Bakken, R. R., Goodwin, E., et al. Antibodies from a human survivor define sites of vulnerability for broad protection against Ebolaviruses Cell 2017 169:878-890 DOI:10.1016/j.cell.2017.04.037 PMID:28525755
- Kumar, R., Ozorowski, G., Kumar, V., Holden, L. G., Shrivastava, T., Patil, S., Deshpande, S., Ward, A. B., Bhattacharya, J. Characterization of a stable HIV-1B/C recombinant, soluble, and trimeric envelope glycoprotein (Env) highly resistant to CD4-induced conformational changes Journal of Biological Chemistry 2017 292:15849-15858 DOI:10.1074/jbc.M117.803056
- Strauch, E. M., Bernard, S. M., La, D., Bohn, A. J., Lee, P. S., Anderson, C. E., Nieusma, T., Holstein, C. A., Garcia, N. K., Hooper, K. A., Ravichandran, R., Nelson, J. W., et al. Computational design of trimeric influenza-neutralizing proteins targeting the hemagglutinin receptor binding site Nature Biotechnology 2017 35:667-671 DOI:10.1038/nbt.3907 PMID:28604661 PMCID:PMC5512607
- Howell, K. A., Brannan, J. M., Bryan, C., McNeal, A., Davidson, E., Turner, H. L., Vu, H., Shulenin, S., He, S., Kuehne, A., Herbert, A. S., Qiu, X., et al. Cooperativity enables non-neutralizing antibodies to neutralize Ebolavirus Cell Reports 2017 19:413-424 DOI:10.1016/j.celrep.2017.03.049 PMID:28402862
- Medina-Ramirez, M., Garces, F., Escolano, A., Skog, P., de Taeye, S. W., Del Moral-Sanchez, I., McGuire, A. T., Yasmeen, A., Behrens, A. J., Ozorowski, G., van den Kerkhof, Tlgm, Freund, N. T., et al. Design and crystal structure of a native-like HIV-1 envelope trimer that engages multiple broadly neutralizing antibody precursors in vivo Journal of Experimental Medicine 2017 214:2573-2590 DOI:10.1084/jem.20161160 PMID:28847869 PMCID:PMC5584115
- Morris, C. D., Azadnia, P., de Val, N., Vora, N., Honda, A., Giang, E., Saye-Francisco, K., Cheng, Y. S., Lin, X., Mann, C. J., Tang, J., Sok, D., et al. Differential antibody responses to conserved HIV-1 neutralizing epitopes in the context of multivalent scaffolds and native-like gp140 trimers MBio 2017 8:e00036-17 DOI:10.1128/mBio.00036-17 PMID:28246356 PMCID:PMC5347340
- Berndsen, Z., Bowman, C., Jang, H., Ward, A. B. EMHP: an accurate automated hole masking algorithm for single-particle cryo-EM image processing Bioinformatics 2017 33:3824-3826 DOI:10.1093/bioinformatics/btx500 PMID:28961740
- Voss, J. E., Andrabi, R., McCoy, L. E., de Val, N., Fuller, R. P., Messmer, T., Su, C. Y., Sok, D., Khan, S. N., Garces, F., Pritchard, L. K., Wyatt, R. T., et al. Elicitation of neutralizing antibodies targeting the V2 apex of the HIV Envelope trimer in a wild-type animal model Cell Reports 2017 21:222-235 DOI:10.1016/j.celrep.2017.09.024 PMID:28978475 PMCID:PMC5640805
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- Ward, A. B., Wilson, I. A. Insights into the trimeric HIV-1 envelope glycoprotein structure Trends in Biochemical Sciences 2015 40:101-107 DOI:10.1016/j.tibs.2014.12.006 PMID:25600289 PMCID:PMC4310573
- Flyak, A. I., Ilinykh, P. A., Murin, C. D., Garron, T., Shen, X., Fusco, M. L., Hashiguchi, T., Bornholdt, Z. A., Slaughter, J. C., Sapparapu, G., Klages, C., Ksiazek, T. G., et al. Mechanism of human antibody-mediated neutralization of Marburg virus Cell 2015 160:893-903 DOI:10.1016/j.cell.2015.01.031 PMID:25723164 PMCID:PMC4344968
- Lee, J. H., de Val, N., Lyumkis, D., Ward, A. B. Model building and refinement of a natively glycosylated HIV-1 Env protein by high-resolution cryoelectron microscopy Structure 2015 23:1943-1951 DOI:10.1016/j.str.2015.07.020 PMID:26388028 PMCID:PMC4618500
- Hu, J. K., Crampton, J. C., Cupo, A., Ketas, T., van Gils, M. J., Sliepen, K., de Taeye, S. W., Sok, D., Ozorowski, G., Deresa, I., Stanfield, R., Ward, A. B., et al. Murine antibody responses to cleaved soluble HIV-1 envelope trimers are highly restricted in specificity Journal of Virology 2015 89:10383-10398 DOI:10.1128/jvi.01653-15 PMID:26246566 PMCID:PMC4580201
- Sliepen, K., Ozorowski, G., Burger, J. A., van Montfort, T., Stunnenberg, M., LaBranche, C., Montefiori, D. C., Moore, J. P., Ward, A. B., Sanders, R. W. Presenting native-like HIV-1 envelope trimers on ferritin nanoparticles improves their immunogenicity Retrovirology 2015 12:82 DOI:10.1186/s12977-015-0210-4 PMID:26410741 PMCID:PMC4583754
- Fusco, M. L., Hashiguchi, T., Cassan, R., Biggins, J. E., Murin, C. D., Warfield, K. L., Li, S., Holtsberg, F. W., Shulenin, S., Vu, H., Olinger, G. G., Kim, D. H., et al. Protective mAbs and cross-reactive mAbs raised by immunization with engineered Marburg virus GPs PLoS Pathogens 2015 11:e1005016 DOI:10.1371/journal.ppat.1005016 PMID:26115029 PMCID:PMC4482612
- Pritchard, L. K., Vasiljevic, S., Ozorowski, G., Seabright, G. E., Cupo, A., Ringe, R., Kim, H. J., Sanders, R. W., Doores, K. J., Burton, D. R., Wilson, I. A., Ward, A. B., et al. Structural constraints determine the glycosylation of HIV-1 envelope trimers Cell Reports 2015 11:1604-1613 DOI:10.1016/j.celrep.2015.05.017 PMID:26051934 PMCID:PMC4555872
- Guenaga, J., de Val, N., Tran, K., Feng, Y., Satchwell, K., Ward, A. B., Wyatt, R. T. Well-ordered trimeric HIV-1 subtype B and C soluble spike mimetics generated by negative selection display native-like properties PLoS Pathogens 2015 11:e1004570 DOI:10.1371/journal.ppat.1004570 PMID:25569572 PMCID:PMC4287557
- Friesen, R. H. E., Lee, P. S., Stoop, E. J. M., Hoffman, R. M. B., Ekiert, D. C., Bhabha, G., Yu, W., Juraszek, J., Koudstaal, W., Jongeneelen, M., Korse, H. J. W. M., Ophorst, C., et al. A common solution to group 2 influenza virus neutralization Proceedings of the National Academy of Sciences of the United States of America 2014 111:445-450 DOI:10.1073/pnas.1319058110 PMID:24335589 PMCID:PMC3890827
- Grover, R. K., Zhu, X., Nieusma, T., Jones, T., Boero, I., MacLeod, A. S., Mark, A., Niessen, S., Kim, H. J., Kong, L., Assad-Garcia, N., Kwon, K., et al. A structurally distinct human mycoplasma protein that generically blocks antigen-antibody union Science 2014 343:656-661 DOI:10.1126/science.1246135 PMID:24503852 PMCID:PMC3987992
- Scharf, L., Scheid, J. F., Lee, J. H., West Jr., A. P., Chen, C., Gao, H., Gnanapragasam, P. N. P., Mares, R., Seaman, M. S., Ward, A. B., Nussenzweig, M. C., Bjorkman, P. J. Antibody 8ANC195 reveals a site of broad vulnerability on the HIV-1 envelope spike Cell Reports 2014 7:785-795 DOI:10.1016/j.celrep.2014.04.001 PMID:24767986 PMCID:PMC4109818
- Huang, J., Kang, B. H., Pancera, M., Lee, J. H., Tong, T., Feng, Y., Imamichi, H., Georgiev, I. S., Chuang, G. Y., Druz, A., Doria-Rose, N. A., Laub, L., et al. Broad and potent HIV-1 neutralization by a human antibody that binds the gp41-gp120 interface Nature 2014 515:138-142 DOI:10.1038/nature13601 PMID:25186731 PMCID:PMC4224615
- Falkowska, E., Le, K. M., Ramos, A., Doores, K. J., Lee, J. H., Blattner, C., Ramirez, A., Derking, R., van Gils, M. J., Liang, C. H., McBride, R., von Bredow, B., et al. Broadly neutralizing HIV antibodies define a glycan-dependent epitope on the prefusion conformation of gp41 on cleaved envelope trimers Immunity 2014 40:657-668 DOI:10.1016/j.immuni.2014.04.009 PMID:24768347 PMCID:PMC4070425
- Tan, G. S., Lee, P. S., Hoffman, R. M. B., Mazel-Sanchez, B., Krammer, F., Leon, P. E., Ward, A. B., Wilson, I. A., Palese, P. Characterization of a broadly neutralizing monoclonal antibody that targets the fusion domain of group 2 influenza A virus hemagglutinin Journal of Virology 2014 88:13580-13592 DOI:10.1128/jvi.02289-14 PMID:25210195 PMCID:PMC4248980
- Doria-Rose, N. A., Schramm, C. A., Gorman, J., Moore, P. L., Bhiman, J. N., DeKosky, B. J., Ernandes, M. J., Georgiev, I. S., Kim, H. J., Pancera, M., Staupe, R. P., Altae-Tran, H. R., et al. Developmental pathway for potent V1V2-directed HIV-neutralizing antibodies Nature 2014 509:55-62 DOI:10.1038/nature13036 PMID:24590074 PMCID:PMC4395007
- Yasmeen, A., Ringe, R., Derking, R., Cupo, A., Julien, J. P., Burton, D. R., Ward, A. B., Wilson, I. A., Sanders, R. W., Moore, J. P., Klasse, P. J. Differential binding of neutralizing and non-neutralizing antibodies to native-like soluble HIV-1 Env trimers, uncleaved Env proteins, and monomeric subunits Retrovirology 2014 11:41 DOI:10.1186/1742-4690-11-41 PMID:24884783 PMCID:PMC4067080
- Matho, M. H., de Val, N., Miller, G. M., Brown, J., Schlossman, A., Meng, X., Crotty, S., Peters, B., Xiang, Y., Hsieh-Wilson, L. C., Ward, A. B., Zajonc, D. M. Murine anti-vaccinia virus D8 antibodies target different epitopes and differ in their ability to block D8 binding to CS-E PLoS Pathogens 2014 10 DOI:10.1371/journal.ppat.1004495 PMID:25474621 PMCID:PMC4256255
- Sok, D., van Gils, M. J., Pauthner, M., Julien, J. P., Saye-Francisco, K. L., Hsueh, J., Briney, B., Lee, J. H., Le, K. M., Lee, P. S., Hua, Y., Seaman, M. S., et al. Recombinant HIV envelope trimer selects for quaternary-dependent antibodies targeting the trimer apex Proceedings of the National Academy of Sciences of the United States of America 2014 111:17624-17629 DOI:10.1073/pnas.1415789111 PMID:25422458 PMCID:PMC4267403
- Ward, A. B. Small-molecule inhibitors: viral fusion arrested Nature Chemical Biology 2014 10:797-798 DOI:10.1038/nchembio.1632 PMID:25229447
- Chung, N. P. Y., Matthews, K., Kim, H. J., Ketas, T. J., Golabek, M., de los Reyes, K., Korzun, J., Yasmeen, A., Sanders, R. W., Klasse, P. J., Wilson, I. A., Ward, A. B., et al. Stable 293 T and CHO cell lines expressing cleaved, stable HIV-1 envelope glycoprotein trimers for structural and vaccine studies Retrovirology 2014 11:33 DOI:10.1186/1742-4690-11-33 PMID:24767177 PMCID:PMC4032163
- Blattner, C., Lee, J. H., Sliepen, K., Derking, R., Falkowska, E., de la Pena, A. T., Cupo, A., Julien, J. P., van Gils, M., Lee, P. S., Peng, W., Paulson, J. C., et al. Structural delineation of a quaternary, cleavage-dependent epitope at the gp41-gp120 interface on intact HIV-1 Env trimers Immunity 2014 40:669-680 DOI:10.1016/j.immuni.2014.04.008 PMID:24768348 PMCID:PMC4057017
- Garces, F., Sok, D., Kong, L., McBride, R., Kim, H. J., Saye-Francisco, K. F., Julien, J. P., Hua, Y., Cupo, A., Moore, J. P., Paulson, J. C., Ward, A. B., et al. Structural evolution of glycan recognition by a family of potent HIV antibodies Cell 2014 159:69-79 DOI:10.1016/j.cell.2014.09.009 PMID:25259921 PMCID:PMC4278586
- Murin, C. D., Julien, J. P., Sok, D., Stanfield, R. L., Khayat, R., Cupo, A., Moore, J. P., Burton, D. R., Wilson, I. A., Ward, A. B. Structure of 2G12 Fab2 in complex with soluble and fully glycosylated HIV-1 Env by negative-stain single-particle electron microscopy Journal of Virology 2014 88:10177-10188 DOI:10.1128/jvi.01229-14 PMID:24965454 PMCID:PMC4136306
- Murin, C. D., Fusco, M. L., Bornholdt, Z. A., Qiu, X., Olinger, G. G., Zeitlin, L., Kobinger, G. P., Ward, A. B., Saphire, E. O. Structures of protective antibodies reveal sites of vulnerability on Ebola virus Proceedings of the National Academy of Sciences of the United States of America 2014 111:17182-17187 DOI:10.1073/pnas.1414164111 PMID:25404321 PMCID:PMC4260551
- Tran, K., Poulsen, C., Guenaga, J., de Val, N., Wilson, R., Sundling, C., Li, Y., Stanfield, R. L., Wilson, I. A., Ward, A. B., Hedestam, G. B. K., Wyatt, R. T. Vaccine-elicited primate antibodies use a distinct approach to the HIV-1 primary receptor binding site informing vaccine redesign Proceedings of the National Academy of Sciences of the United States of America 2014 111:E738-E747 DOI:10.1073/pnas.1319512111 PMID:24550318 PMCID:PMC3932900
- Sanders, R. W., Derking, R., Cupo, A., Julien, J. P., Yasmeen, A., de Val, N., Kim, H. J., Blattner, C., de la Pena, A. T., Korzun, J., Golabek, M., de los Reyes, K., et al. A next-generation cleaved, soluble HIV-1 Env Trimer, BG505 SOSIP.664 gp140, expresses multiple epitopes for broadly neutralizing but not non-neutralizing antibodies PLoS Pathogens 2013 9:e1003618 DOI:10.1371/journal.ppat.1003618 PMID:24068931 PMCID:PMC3777863
- Hong, M., Lee, P. S., Hoffman, R. M. B., Zhu, X., Krause, J. C., Laursen, N. S., Yoon, S. I., Song, L., Tussey, L., Crowe, J. E., Ward, A. B., Wilson, I. A. Antibody recognition of the pandemic H1N1 Influenza virus hemagglutinin receptor binding site Journal of Virology 2013 87:12471-12480 DOI:10.1128/jvi.01388-13 PMID:24027321 PMCID:PMC3807900
- Julien, J. P., Lee, J. H., Cupo, A., Murin, C. D., Derking, R., Hoffenberg, S., Caulfield, M. J., King, C. R., Marozsan, A. J., Klasse, P. J., Sanders, R. W., Moore, J. P., et al. Asymmetric recognition of the HIV-1 trimer by broadly neutralizing antibody PG9 Proceedings of the National Academy of Sciences of the United States of America 2013 110:213-218 DOI:10.1073/pnas.1217537110 PMID:23426631 PMCID:PMC3600498
- Ward, A. B., Sali, A., Wilson, I. A. Biochemistry. Integrative structural biology Science 2013 339:913-915 DOI:10.1126/science.1228565 PMID:23430643 PMCID:PMC3633482
- Julien, J. P., Sok, D., Khayat, R., Lee, J. H., Doores, K. J., Walker, L. M., Ramos, A., Diwanji, D. C., Pejchal, R., Cupo, A., Katpally, U., Depetris, R. S., et al. Broadly neutralizing antibody PGT121 allosterically modulates CD4 binding via recognition of the HIV-1 gp120 V3 base and multiple surrounding glycans PLoS Pathogens 2013 9:e1003342 DOI:10.1371/journal.ppat.1003342 PMID:23658524 PMCID:PMC3642082
- Ringe, R. P., Sanders, R. W., Yasmeen, A., Kim, H. J., Lee, J. H., Cupo, A., Korzun, J., Derking, R., van Montfort, T., Julien, J. P., Wilson, I. A., Klasse, P. J., et al. Cleavage strongly influences whether soluble HIV-1 envelope glycoprotein trimers adopt a native-like conformation Proceedings of the National Academy of Sciences of the United States of America 2013 110:18256-18261 DOI:10.1073/pnas.1314351110 PMID:24145402 PMCID:PMC3831437
- Lyumkis, D., Julien, J. P., de Val, N., Cupo, A., Potter, C. S., Klasse, P. J., Burton, D. R., Sanders, R. W., Moore, J. P., Carragher, B., Wilson, I. A., Ward, A. B. Cryo-EM structure of a fully glycosylated soluble cleaved HIV-1 envelope trimer Science 2013 342:1484-1490 DOI:10.1126/science.1245627 PMID:24179160 PMCID:PMC3954647
- Julien, J. P., Cupo, A., Sok, D., Stanfield, R. L., Lyumkis, D., Deller, M. C., Klasse, P. J., Burton, D. R., Sanders, R. W., Moore, J. P., Ward, A. B., Wilson, I. A. Crystal structure of a soluble cleaved HIV-1 envelope trimer Science 2013 342:1477-1483 DOI:10.1126/science.1245625 PMID:24179159 PMCID:PMC3886632
- Kong, L., Giang, E., Nieusma, T., Kadam, R. U., Cogburn, K. E., Hua, Y., Dai, X., Stanfield, R. L., Burton, D. R., Ward, A. B., Wilson, I. A., Law, M. Hepatitis C virus E2 envelope glycoprotein core structure Science 2013 342:1090-1094 DOI:10.1126/science.1243876 PMID:24288331 PMCID:PMC3954638
- Thornburg, N. J., Nannemann, D. P., Blum, D. L., Belser, J. A., Tumpey, T. M., Deshpande, S., Fritz, G. A., Sapparapu, G., Krause, J. C., Lee, J. H., Ward, A. B., Lee, D. E., et al. Human antibodies that neutralize respiratory droplet transmissible H5N1 influenza viruses Journal of Clinical Investigation 2013 123:4405-4409 DOI:10.1172/jci69377 PMID:23999429 PMCID:PMC3784541
- Klasse, P. J., Depetris, R. S., Pejchal, R., Julien, J. P., Khayat, R., Lee, J. H., Marozsan, A. J., Cupo, A., Cocco, N., Korzun, J., Yasmeen, A., Ward, A. B., et al. Influences on Trimerization and Aggregation of Soluble, Cleaved HIV-1 SOSIP Envelope Glycoprotein Journal of Virology 2013 87:9873-9885 DOI:10.1128/jvi.01226-13 PMID:23824824 PMCID:PMC3754145
- Jardine, J., Julien, J. P., Menis, S., Ota, T., Kalyuzhniy, O., McGuire, A., Sok, D., Huang, P. S., MacPherson, S., Jones, M., Nieusma, T., Mathison, J., et al. Rational HIV immunogen design to target specific germline B cell receptors Science 2013 340:711-716 DOI:10.1126/science.1234150 PMID:23539181 PMCID:PMC3689846
- Lee, S. C., Bennett, B. C., Hong, W. X., Fu, Y., Baker, K. A., Marcoux, J., Robinson, C. V., Ward, A. B., Halpert, J. R., Stevens, R. C., Stout, C. D., Yeager, M. J., et al. Steroid-based facial amphiphiles for stabilization and crystallization of membrane proteins Proceedings of the National Academy of Sciences of the United States of America 2013 110:E1203-11 DOI:10.1073/pnas.1221442110 PMID:23479627 PMCID:PMC3612649
- Wu, Y., West, A. P., Kim, H. J., Thornton, M. E., Ward, A. B., Bjorkman, P. J. Structural basis for enhanced HIV-1 neutralization by a dimeric immunoglobulin G form of the glycan-recognizing antibody 2G12 Cell Reports 2013 5:1443-1455 DOI:10.1016/j.celrep.2013.11.015 PMID:24316082 PMCID:PMC3919625
- Khayat, R., Lee, J. H., Julien, J. P., Cupo, A., Klasse, P. J., Sanders, R. W., Moore, J. P., Wilson, I. A., Ward, A. B. Structural characterization of cleaved, soluble HIV-1 envelope glycoprotein trimers Journal of Virology 2013 87:9865-9872 DOI:10.1128/jvi.01222-13 PMID:23824817 PMCID:PMC3754114
- Ward, A. B., Szewczyk, P., Grimard, V., Lee, C. W., Martinez, L., Doshi, R., Caya, A., Villaluz, M., Pardon, E., Cregger, C., Swartz, D. J., Falson, P. G., et al. Structures of P-glycoprotein reveal its conformational flexibility and an epitope on the nucleotide-binding domain Proceedings of the National Academy of Sciences of the United States of America 2013 110:13386-13391 DOI:10.1073/pnas.1309275110 PMID:23901103 PMCID:PMC3746859
- Kong, L., Lee, J. H., Doores, K. J., Murin, C. D., Julien, J. P., McBride, R., Liu, Y., Marozsan, A., Cupo, A., Klasse, P. J., Hoffenberg, S., Caulfield, M., et al. Supersite of immune vulnerability on the glycosylated face of HIV-1 envelope glycoprotein gp120 Nature Structural & Molecular Biology 2013 20:796-803 DOI:10.1038/nsmb.2594 PMID:23708606 PMCID:PMC3823233
- Burton, D. R., Ahmed, R., Barouch, D. H., Butera, S. T., Crotty, S., Godzik, A., Kaufmann, D. E., McElrath, M. J., Nussenzweig, M. C., Pulendran, B., Scanlan, C. N., Schief, W. R., et al. A blueprint for HIV vaccine discovery Cell Host & Microbe 2012 12:396-407 DOI:10.1016/j.chom.2012.09.008 PMID:23084910 PMCID:PMC3513329
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Ward, A. B., Guvench, O., Hills, R. D.
Coarse grain lipid-protein molecular interactions and diffusion with MsbA flippase
Proteins-Structure Function and Bioinformatics
2012
80:2178-2190
DOI:10.1002/prot.24108
PMID:22552999
- Ekiert, D. C., Kashyap, A. K., Steel, J., Rubrum, A., Bhabha, G., Khayat, R., Lee, J. H., Dillon, M. A., O'Neil, R. E., Faynboym, A. M., Horowitz, M., Horowitz, L., et al. Cross-neutralization of influenza a viruses mediated by a single antibody loop Nature 2012 489:526-32 DOI:10.1038/nature11414 PMID:22982990 PMCID:PMC3538848
- Dreyfus, C., Laursen, N. S., Kwaks, T., Zuijdgeest, D., Khayat, R., Ekiert, D. C., Lee, J. H., Metlagel, Z., Bujny, M. V., Jongeneelen, M., van der Vlugt, R., Lamrani, M., et al. Highly conserved protective epitopes on influenza B viruses Science 2012 337:1343-1348 DOI:10.1126/science.1222908 PMID:22878502 PMCID:PMC3538841
- Depetris, R. S., Julien, J. P., Khayat, R., Lee, J. H., Pejchal, R., Katpally, U., Cocco, N., Kachare, M., Massi, E., David, K. B., Cupo, A., Marozsan, A. J., et al. Partial enzymatic deglycosylation preserves the structure of cleaved recombinant HIV-1 envelope glycoprotein trimers Journal of Biological Chemistry 2012 287:24239-24254 DOI:10.1074/jbc.M112.371898 PMID:22645128 PMCID:PMC3397850
- Bai, J. P., Swartz, D. J., Protasevich, II, Brouillette, C. G., Harrell, P. M., Hildebrandt, E., Gasser, B., Mattanovich, D., Ward, A., Chang, G., Urbatsch, I. L. A gene optimization strategy that enhances production of fully functional p-glycoprotein in pichia pastoris PLoS One 2011 6:e22577 DOI:10.1371/journal.pone.0022577 PMID:21826197 PMCID:PMC3149604
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Fisher, L. S., Ward, A., Milligan, R. A., Unwin, N., Potter, C. S., Carragher, B.
A helical processing pipeline for EM structure determination of membrane proteins
Methods
2011
55:350-362
DOI:10.1016/j.ymeth.2011.09.013
PMID:21964395
PMCID:PMC3262078
- Pejchal, R., Doores, K. J., Walker, L. M., Khayat, R., Huang, P. S., Wang, S. K., Stanfield, R. L., Julien, J. P., Ramos, A., Crispin, M., Depetris, R., Katpally, U., et al. A potent and broad neutralizing antibody recognizes and penetrates the HIV glycan shield Science 2011 334:1097-1103 DOI:10.1126/science.1213256 PMID:21998254 PMCID:PMC3280215
- McLellan, J. S., Pancera, M., Carrico, C., Gorman, J., Julien, J. P., Khayat, R., Louder, R., Pejchal, R., Sastry, M., Dai, K. F., O'Dell, S., Patel, N., et al. Structure of HIV-1 gp120 V1/V2 domain with broadly neutralizing antibody PG9 Nature 2011 480:336-343 DOI:10.1038/nature10696 PMID:22113616 PMCID:PMC3406929
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Perz-Edwards, R. J., Irving, T. C., Baumann, B. A. J., Gore, D., Hutchinson, D. C., Krzic, U., Porter, R. L., Ward, A. B., Reedy, M. K.
X-ray diffraction evidence for myosin-troponin connections and tropomyosin movement during stretch activation of insect flight muscle
Proceedings of the National Academy of Sciences of the United States of America
2011
108:120-125
DOI:10.1073/pnas.1014599107
PMID:21148419
PMCID:PMC3017141
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Glaves, J. P., Fisher, L., Ward, A., Young, H. S.
Helical crystallization of two example membrane proteins MsbA and the Ca(2+)-ATPase
Methods in Enzymology
2010
483:143-159
DOI:10.1016/s0076-6879(10)83007-1
PMID:20888473
- Gutmann, D. A. P., Ward, A., Urbatsch, I. L., Chang, G., van Veen, H. W. Understanding polyspecificity of multidrug ABC transporters: closing in on the gaps in ABCB1 Trends in Biochemical Sciences 2010 35:36-42 DOI:10.1016/j.tibs.2009.07.009 PMID:19819701 PMCID:PMC4608440
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Ward, A., Mulligan, S., Carragher, B., Chang, G., Milligan, R. A.
Nucleotide dependent packing differences in helical crystals of the ABC transporter MsbA
Journal of Structural Biology
2009
165:169-175
DOI:10.1016/j.jsb.2008.11.006
PMID:19114108
PMCID:PMC2703300
- Aller, S. G., Yu, J., Ward, A., Weng, Y., Chittaboina, S., Zhuo, R. P., Harrell, P. M., Trinh, Y. T., Zhang, Q. H., Urbatsch, I. L., Chang, G. Structure of P-glycoprotein reveals a molecular basis for poly-specific drug binding Science 2009 323:1718-1722 DOI:10.1126/science.1168750 PMID:19325113 PMCID:PMC2720052
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Bekyarova, T. I., Reedy, M. C., Baumann, B. A. J., Tregear, R. T., Ward, A., Krzic, U., Prince, K. M., Perz-Edwards, R. J., Reconditi, M., Gore, D., Irving, T. C., Reedy, M. K.
Reverse actin sliding triggers strong myosin binding that moves tropomyosin
Proceedings of the National Academy of Sciences of the United States of America
2008
105:10372-10377
DOI:10.1073/pnas.0709877105
PMID:18658238
PMCID:PMC2492518
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Littlefield, K. P., Ward, A. B., Chappie, J. S., Reedy, M. K., Bernstein, S. I., Milligan, R. A., Reedy, M. C.
Similarities and differences between frozen-hydrated, rigor acto-S1 complexes of insect flight and chicken skeletal muscles
Journal of Molecular Biology
2008
381:519-528
DOI:10.1016/j.jmb.2008.06.029
PMID:18588896
PMCID:PMC2567842
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Zhang, Q. H., Ma, X. Q., Ward, A., Hong, W. X., Jaakola, V. P., Stevens, R. C., Finn, M. G., Chang, G.
Designing facial amphiphiles for the stabilization of integral membrane proteins
Angewandte Chemie-International Edition
2007
46:7023-7025
DOI:10.1002/anie.200701556
PMID:17691085
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Ward, A., Reyes, C. L., Yu, J., Roth, C. B., Chang, G.
Flexibility in the ABC transporter MsbA: Alternating access with a twist
Proceedings of the National Academy of Sciences of the United States of America
2007
104:19005-19010
DOI:10.1073/pnas.0709388104
PMID:18024585
PMCID:PMC2141898
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Reyes, C. L., Ward, A., Yu, J., Chang, G.
The structures of MsbA: Insight into ABC transporter-mediated multidrug efflux
FEBS Letters
2006
580:1042-1048
DOI:10.1016/j.febslet.2005.11.033
PMID:16337944
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Ward, A., Moody, M. F., Sheehan, B., Milligan, R. A., Carragher, B.
Windex: A toolset for indexing helices
Journal of Structural Biology
2003
144:172-183
DOI:10.1016/j.jsb.2003.10.008
PMID:14643220
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- Andrew Ward Wins Ray Thomas Edwards Foundation Award Newsletter
- Consortium Led by Scripps Research Institute Scientist Wins Up to $28 Million from NIH to Find Best Proposed Ebola Treatment News Release
- New Vaccine-Design Approach Targets HIV and Other Fast-Mutating Viruses News Release
- Scientists Capture Most Detailed Picture Yet of Key AIDS Protein News Release
- Scientists Reveal Surprising Picture of How Powerful Antibody Neutralizes HIV News Release
- Scripps Research Institute Scientists Achieve Most Detailed Picture Ever of Key Part of Hepatitis C Virus News Release
- TSRI Named ‘Top Institution’ in HIV/AIDS Research; Dennis Burton, ‘Hot Researcher’ Newsletter
- The Ultimate Decoy: Scripps Research Institute Scientists Find Protein that Helps Bacteria Misdirect Immune System News Release
