Shen, Ben

Faculty Member

Positions

2017 - co-Chair, Chemistry , Scripps Research
2017 - Professor (Joint Appointment), Molecular Medicine , Scripps Research
2011 - Professor, Chemistry , Scripps Research
2011 - Faculty Member, Skaggs Graduate School of Chemical and Biological Sciences , Scripps Research
2011 - Professor (Joint Appointment), The Worm Institute for Research and Medicine (WIRM) , Scripps Research
2011 - 2017 Professor (Joint Appointment), Molecular Therapeutics , Scripps Research
2005 - 2010 Principal Investigator, NCI UW National Cooperative Drug Discovery Group, University of Wisconsin-Madison
2004 - 2010 School of Pharmacy and Department of Chemistry, University of Wisconsin-Madison
2004 - 2010 Charles M. Johnson Distinguished Chair, School of Pharmacy, University of Wisconsin-Madison
2001 - 2003 Associate Professor, School of Pharmacy and Department of Chemistry, University of Wisconsin-Madison
1995 - 2001 Assistant Professor, Department of Chemistry, University of California, Davis
1991 - 1995 Postdoctoral Research Associate, University of Wisconsin-Madison

Research Areas

Drug Discovery (UMLS)

Contact

shenb@scripps.edu

Websites

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Publications without PubMed PMIDs

academic article
- Liang, H., Jiang, L., Jiang, Q., Shi, J., Xiang, J., Yan, X., Zhu, X., Zhao, L., Shen, B., Duan, Y., Huang, Y. A 3-hydroxy-3-methylglutaryl-CoA synthase-based probe for the discovery of the acyltransferase-less type I polyketide synthases Environmental Microbiology 2019 DOI:10.1111/1462-2920.14787 PMID:31435987
- Teijaro, C. N., Adhikari, A., Shen, B. Challenges and opportunities for natural product discovery, production, and engineering in native producers versus heterologous hosts Journal of Industrial Microbiology & Biotechnology 2019 46:433-444 DOI:10.1007/s10295-018-2094-5 PMID:30426283 PMCID:PMC6405299
- Dong, L. B., Liu, Y. C., Cepeda, A. J., Kalkreuter, E., Deng, M. R., Rudolf, J. D., Chang, C., Joachimiak, A., Phillips, G. N., Shen, B. Characterization and crystal structure of a nonheme diiron monooxygenase involved in platensimycin and platencin biosynthesis Journal of the American Chemical Society 2019 141:12406-12412 DOI:10.1021/jacs.9b06183 PMID:31291107
- Dong, L. B., Zhang, X., Rudolf, J. D., Deng, M. R., Kalkreuter, E., Cepeda, A. J., Renata, H., Shen, B. Cryptic and stereospecific hydroxylation, oxidation, and reduction in platensimycin and platencin biosynthesis Journal of the American Chemical Society 2019 141:4043-4050 DOI:10.1021/jacs.8b13452 PMID:30735041
- Xu, Z., Fang, S. M., Bakowski, M. A., Rateb, M. E., Yang, D., Zhu, X., Huang, Y., Zhao, L. X., Jiang, Y., Duan, Y., Hull, M. V., McNamara, C. W., et al. Discovery of kirromycins with anti-Wolbachia activity from Streptomyces sp. CB00686 ACS Chemical Biology 2019 14:1174-1182 DOI:10.1021/acschembio.9b00086 PMID:31074963
- Su, M., Qiu, L., Deng, Y., Ruiz, C. H., Rudolf, J. D., Dong, L. B., Feng, X., Cameron, M. D., Shen, B., Duan, Y., Huang, Y. Evaluation of platensimycin and platensimycin-inspired thioether analogues against methicillin-resistant staphylococcus aureus in topical and systemic infection mouse models Molecular Pharmaceutics 2019 16:3065-3071 DOI:10.1021/acs.molpharmaceut.9b00293 PMID:31244223
- Deng, Y., Weng, X., Li, Y., Su, M., Wen, Z., Ji, X., Ren, N., Shen, B., Duan, Y., Huang, Y. Late-stage functionalization of platensimycin leading to multiple analogues with improved antibacterial activity in vitro and in vivo Journal of Medicinal Chemistry 2019 62:6682-6693 DOI:10.1021/acs.jmedchem.9b00616 PMID:31265289
- Qiu, L., Wen, Z., Li, Y., Tian, K., Deng, Y., Shen, B., Duan, Y., Huang, Y. Stereoselective functionalization of platensimycin and platencin by sulfa-Michael/aldol reactions Organic & Biomolecular Chemistry 2019 17:4261-4272 DOI:10.1039/c9ob00324j PMID:30816397
- Wang, Z., Wen, Z., Liu, L., Zhu, X., Shen, B., Yan, X., Duan, Y., Huang, Y. Yangpumicins F and G, enediyne congeners from Micromonospora yangpuensis DSM 45577 Journal of Natural Products 2019 82:2483-2488 DOI:10.1021/acs.jnatprod.9b00229 PMID:31490685
- Crnovcic, I., Gan, F., Yang, D., Dong, L. B., Schultz, P. G., Shen, B. Activities of recombinant human bleomycin hydrolase on bleomycins and engineered analogues revealing new opportunities to overcome bleomycin-induced pulmonary toxicity Bioorganic & Medicinal Chemistry Letters 2018 28:2670-2674 DOI:10.1016/j.bmcl.2018.04.065 PMID:29730026
- Annaval, T., Han, L., Rudolf, J. D., Xie, G., Yang, D., Chang, C. Y., Ma, M., Crnovcic, I., Miller, M. D., Soman, J., Xu, W., Phillips Jr., G. N., et al. Biochemical and structural characterization of TtnD, a prenylated FMN-dependent decarboxylase from the tautomycetin biosynthetic pathway ACS Chemical Biology 2018 13:2728-2738 DOI:10.1021/acschembio.8b00673 PMID:30152678
- Qu, L., Tian, K., Wen, Z., Deng, Y., Kang, D., Liang, H., Zhu, X., Shen, B., Duan, Y., Huang, Y. Biomimetic stereoselective sulfa-michael addition leads to platensimycin and platencin sulfur analogues against methicillin-resistant Staphylococcus aureus Journal of Natural Products 2018 81:316-322 DOI:10.1021/acs.jnatprod.7b00745 PMID:29389125
- Dong, L. B., Rudolf, J. D., Kang, D., Wang, N., He, C. Q., Deng, Y., Huang, Y., Houk, K. N., Duan, Y., Shen, B. Biosynthesis of thiocarboxylic acid-containing natural products Nature Communications 2018 9 DOI:10.1038/s41467-018-04747-y PMID:29915173 PMCID:PMC6006322
- Kearney, S. E., Zahoranszky-Kohalmi, G., Brimacombe, K. R., Henderson, M. J., Lynch, C., Zhao, T., Wan, K. K., Itkin, Z., Dillon, C., Shen, M., Cheff, D. M., Lee, T. D., et al. Canvass: a crowd-sourced, natural-product screening library for exploring biological space ACS Central Science 2018 4:1727-1741 DOI:10.1021/acscentsci.8b00747 PMID:30648156 PMCID:PMC6311695
- Yan, X., Chen, J. J., Adhikari, A., Teijaro, C. N., Ge, H., Crnovcic, I., Chang, C. Y., Annaval, T., Yang, D., Rader, C., Shen, B. Comparative studies of the biosynthetic gene clusters for anthraquinone-fused enediynes shedding light into the tailoring steps of tiancimycin biosynthesis Organic Letters 2018 20:5918-5921 DOI:10.1021/acs.orglett.8b02584 PMID:30212211
- Rudolf, J. D., Dong, L. B., Zhang, X., Renata, H., Shen, B. Cytochrome P450-catalyzed hydroxylation initiating ether formation in platensimycin biosynthesis Journal of the American Chemical Society 2018 140:12349-12353 DOI:10.1021/jacs.8b08012 PMID:30216060
- Xu, Z., Pan, G., Zhou, H., Shen, B. Discovery and characterization of 1-aminocyclopropane-1-carboxylic acid synthase of bacterial origin Journal of the American Chemical Society 2018 140:16957-16961 DOI:10.1021/jacs.8b11463 PMID:30472830
- Yan, X., Hindra, Ge, H., Yang, D., Huang, T., Crnovcic, I., Chang, C. Y., Fang, S. M., Annaval, T., Zhu, X., Huang, Y., Zhao, L. X., et al. Discovery of alternative producers of the enediyne antitumor antibiotic C-1027 with high titers Journal of Natural Products 2018 81:594-599 DOI:10.1021/acs.jnatprod.7b01013 PMID:29345939
- Dong, L. B., Rudolf, J. D., Deng, M. R., Yan, X., Shen, B. Discovery of the tiancilactone antibiotics by genome mining of atypical bacterial type II diterpene synthases ChemBioChem 2018 19:1727-1733 DOI:10.1002/cbic.201800285 PMID:29806086
- Yang, D., Hindra, Dong, L. B., Crnovcic, I., Shen, B. Engineered production and evaluation of 6'-deoxy-tallysomycin H-1 revealing new insights into the structure-activity relationship of the anticancer drug bleomycin Journal of Antibiotics 2018 71:97-103 DOI:10.1038/ja.2017.93 PMID:28831149
- Chen, J. J., Rateb, M. E., Love, M. S., Xu, Z., Yang, D., Zhu, X., Huang, Y., Zhao, L. X., Jiang, Y., Duan, Y., McNamara, C. W., Shen, B. Herbicidins from Streptomyces sp. CB01388 showing anti-cryptosporidium activity Journal of Natural Products 2018 81:791-797 DOI:10.1021/acs.jnatprod.7b00850 PMID:29469575
- Jiang, L., Pu, H., Xiang, J., Su, M., Yan, X., Yang, D., Zhu, X., Shen, B., Duan, Y., Huang, Y. Huanglongmycin A-C, cytotoxic polyketides biosynthesized by a putative type II polyketide synthase from Streptomyces sp. CB09001 Frontiers in Chemistry 2018 6 DOI:10.3389/fchem.2018.00254 PMID:30013965 PMCID:PMC6036704
- Wang, N., Rudolf, J. D., Dong, L. B., Osipiuk, J., Hatzos-Skintges, C., Endres, M., Chang, C. Y., Babnigg, G., Joachimiak, A., Phillips, G. N., Shen, B. Natural separation of the acyl-CoA ligase reaction results in a non-adenylating enzyme Nature Chemical Biology 2018 14:730-737 DOI:10.1038/s41589-018-0061-0 PMID:29867143 PMCID:PMC6008203
- Nejad, M. I., Yang, D., Shen, B., Gates, K. S. Oxidative activation of leinamycin E1 triggers alkylation of guanine residues in double-stranded DNA Chemical Communications 2018 54:256-259 DOI:10.1039/c7cc08482j PMID:29231931
- Kwong, T., Ma, M., Pan, G., Hindra, Yang, D., Yang, C., Lohman, J. R., Rudolf, J. D., Cleveland, J. L., Shen, B. P450-catalyzed tailoring steps in leinamycin biosynthesis featuring regio- and stereoselective hydroxylations and substrate promiscuities Biochemistry 2018 57:5005-5013 DOI:10.1021/acs.biochem.8b00623 PMID:30070831 PMCID:PMC6211295
- Guo, X., Crnovcic, I., Chang, C. Y., Luo, J., Lohman, J. R., Papinski, M., Bechthold, A., Horsman, G. P., Shen, B. PokMT1 from the polyketomycin biosynthetic machinery of Streptomyces diastatochromogenes Tü6028 belongs to the emerging family of C-methyltransferases that act on CoA-activated aromatic substrates Biochemistry 2018 57:1003-1011 DOI:10.1021/acs.biochem.7b01219
- Chang, C. Y., Yan, X., Crnovcic, I., Annaval, T., Chang, C., Nocek, B., Rudolf, J. D., Yang, D., Hindra, Babnigg, G., Joachimiak, A., Phillips, G. N., et al. Resistance to enediyne antitumor antibiotics by sequestration Cell Chemical Biology 2018 25:1075-1085 DOI:10.1016/j.chembiol.2018.05.012 PMID:29937405
- Liu, L., Pan, J., Wang, Z., Yan, X., Yang, D., Zhu, X., Shen, B., Duan, Y., Huang, Y. Ribosome engineering and fermentation optimization leads to overproduction of tiancimycin A, a new enediyne natural product from Streptomyces sp CB03234 Journal of Industrial Microbiology & Biotechnology 2018 45:141-151 DOI:10.1007/s10295-018-2014-8 PMID:29396746
- Tian, K., Deng, Y., Qiu, L., Zhu, X., Shen, B., Duan, Y., Huang, Y. Semisynthesis and biological evaluation of platensimycin analogues with varying aminobenzoic acids ChemistrySelect 2018 3:12625-12629 DOI:10.1002/slct.201802475
- Den, Y., Su, M., Kang, D., Liu, X., Wen, Z., Li, Y., Qiu, L., Shen, B., Duan, Y., Huang, Y. Semisynthesis of platensimycin derivatives with antibiotic activities in mice via Suzuki-Miyaura cross-coupling reactions Journal of Medicinal Chemistry 2018 61:11341-11348 DOI:10.1021/acs.jmedchem.8b01580 PMID:30461269
- Zhu, X., Kong, J., Yang, H., Huang, R., Huang, Y., Yang, D., Shen, B., Duan, Y. Strain improvement by combined UV mutagenesis and ribosome engineering and subsequent fermentation optimization for enhanced 6 '-deoxy-bleomycin Z production Applied Microbiology and Biotechnology 2018 102:1651-1661 DOI:10.1007/s00253-017-8705-7 PMID:29279956
- Chang, C. Y., Lohman, J. R., Huang, T., Michalska, K., Bigelow, L., Rudolf, J. D., Jedrzejczak, R., Yan, X., Ma, M., Babnigg, G., Joachimiak, A., Phillips, G. N., et al. Structural insights into the free-standing condensation enzyme SgcC5 catalyzing ester-bond formation in the biosynthesis of the enediyne antitumor antibiotic C-1027 Biochemistry 2018 57:3278-3288 DOI:10.1021/acs.biochem.8b00174 PMID:29533601
- Kong, J. Q., Yi, L., Xiong, Y., Huang, Y., Yang, D., Yan, X., Shen, B., Duan, Y., Zhu, X. The discovery and development of microbial bleomycin analogues Applied Microbiology and Biotechnology 2018 102:6791-6798 DOI:10.1007/s00253-018-9129-8 PMID:29876605
- Murray, V., Chen, J. K., Yang, D., Shen, B. The genome-wide sequence specificity of DNA cleavage by bleomycin analogues in human cells Bioorganic & Medicinal Chemistry 2018 26:4168-4178 DOI:10.1016/j.bmc.2018.07.006 PMID:30006142
- Deng, Y., Kang, D., Shi, J., Zhou, W., Sun, A. J., Ju, J., Zhu, X., Shen, B., Duan, Y., Huang, Y. The semi-synthesis, biological evaluation and docking analysis of the oxime, hydrazine and hydrazide derivatives of platensimycin MedChemComm 2018 9:789-794 DOI:10.1039/c8md00081f
- Qiu, L., Tian, K., Pan, J., Jiang, L., Yang, H., Zhu, X., Shen, B., Duan, Y., Huang, Y. A facile semi-synthetic approach towards halogen-substituted aminobenzoic acid analogues of platensimycin Tetrahedron 2017 73:771-775 DOI:10.1016/j.tet.2016.12.059
- Zhang, B., Xu, Z., Teng, Q., Pan, G., Ma, M., Shen, B. A long-range acting dehydratase domain as the missing link for C17-dehydration in iso-migrastatin biosynthesis Angewandte Chemie-International Edition 2017 56:7247-7251 DOI:10.1002/anie.201703588 PMID:28464455
- Chen, J. K., Yang, D., Shen, B., Murray, V. Bleomycin analogues preferentially cleave at the transcription start sites of actively transcribed genes in human cells International Journal of Biochemistry & Cell Biology 2017 85:56-65 DOI:10.1016/j.biocel.2017.02.001 PMID:28167289
- Yang, D., Rateb, M. E., Wang, N., Shen, B. Competition and co-regulation of spirotoamide and tautomycetin biosynthesis in Streptomyces griseochromogenes, and isolation and structural elucidation of spirotoamide C and D Journal of Antibiotics 2017 70:710-714 DOI:10.1038/ja.2017.13 PMID:28196980
- Rudolf, J. D., Chang, C. Y., Ma, M., Shen, B. Cytochromes P450 for natural product biosynthesis in Streptomyces: sequence, structure, and function Natural Product Reports 2017 34:1141-1172 DOI:10.1039/c7np00034k PMID:28758170 PMCID:PMC5585785
- Pan, G., Xu, Z., Guo, Z., Hindra, Ma, M., Yang, D., Zhou, H., Gansemans, Y., Zhu, X., Huang, Y., Zhao, L. X., Jiang, Y., et al. Discovery of the leinamycin family of natural products by mining actinobacterial genomes Proceedings of the National Academy of Sciences of the United States of America 2017 114:E11131-E11140 DOI:10.1073/pnas.1716245115 PMID:29229819 PMCID:PMC5748217
- Yan, X., Chen, J. J., Adhikari, A., Yang, D., Crnovcic, I., Wang, N., Chang, C. Y., Rader, C., Shen, B. Genome mining of micromonospora yangpuensis DSM 45577 as a producer of an anthraquinone-fused enediyne Organic Letters 2017 19:6192-6195 DOI:10.1021/acs.orglett.7b03120 PMID:29086572
- Hindra, Yang, D., Teng, Q., Dong, L. B., Crnovcic, I., Huang, T., Ge, H., Shen, B. Genome mining of streptomyces mobaraensis DSM40847 as a bleomycin producer providing a biotechnology platform to engineer designer bleomycin analogues Organic Letters 2017 19:1386-1389 DOI:10.1021/acs.orglett.7b00283 PMID:28256838
- Ma, M., Rateb, M. E., Yang, D., Rudolf, J. D., Zhu, X., Huang, Y., Zhao, L. X., Jiang, Y., Duan, Y., Shen, B. Germicidins H-J from streptomyces sp CB00361 Journal of Antibiotics 2017 70:200-203 DOI:10.1038/ja.2016.100
- Dong, L. B., Rudolf, J. D., Lin, L., Ruiz, C., Cameron, M. D., Shen, B. In vivo instability of platensimycin and platencin: Synthesis and biological evaluation of urea- and carbamate-platensimycin Bioorganic & Medicinal Chemistry 2017 25:1990-1996 DOI:10.1016/j.bmc.2017.02.028 PMID:28237556
- Guo, Z., Pan, G., Xu, Z., Yang, D., Hindra, Zhu, X., Huang, Y., Zhao, L. X., Jiang, Y., Duan, Y., Shen, B. New isofuranonaphthoquinones and isoindolequinones from Streptomyces sp. CB01883 Journal of Antibiotics 2017 70:414-422 DOI:10.1038/ja.2016.122 PMID:27731333
- Falzone, M., Crespo, E., Jones, K., Khan, G., Korn, V. L., Patel, A., Patel, M., Patel, K., Perkins, C., Siddiqui, S., Stenger, D., Yu, E., et al. Nutritional control of antibiotic production by Streptomyces platensis MA7327: importance of L-aspartic acid Journal of Antibiotics 2017 70:828-831 DOI:10.1038/ja.2017.49 PMID:28465627
- Rudolf, J. D., Dong, L. B., Shen, B. Platensimycin and platencin: Inspirations for chemistry, biology, enzymology, and medicine Biochemical Pharmacology 2017 133:139-151 DOI:10.1016/j.bcp.2016.11.013 PMID:27865713 PMCID:PMC5410400
- Dong, L. B., Rudolf, J. D., Shen, B. A mutasynthetic library of platensimycin and platencin analogues Organic Letters 2016 18:4606-4609 DOI:10.1021/acs.orglett.6602248 PMID:27599271 PMCID:PMC5048679
- Dong, L. B., Rudolf, J. D., Shen, B. Antibacterial sulfur-containing platensimycin and platencin congeners from Streptomyces platensis SB12029 Bioorganic & Medicinal Chemistry 2016 24:6348-6353 DOI:10.1016/j.bmc.2016.04.026 PMID:27134119 PMCID:PMC5063666
- Rudolf, J. D., Dong, L. B., Manoogian, K., Shen, B. Biosynthetic origin of the ether ring in platensimycin Journal of the American Chemical Society 2016 138:16711-16721 DOI:10.1021/jacs.6b09818 PMID:27966343
- Huang, Y., Yang, D., Pan, G., Tang, G. L., Shen, B. Characterization of LnmO as a pathway-specific Crp/Fnr-type positive regulator for leinamycin biosynthesis in Streptomyces atroolivaceus and its application for titer improvement Applied Microbiology and Biotechnology 2016 100:10555-10562 DOI:10.1007/s00253-016-7864-2 PMID:27704182 PMCID:PMC5121087
- Huang, Y., Tang, G. L., Pan, G., Chang, C. Y., Shen, B. Characterization of the ketosynthase and acyl carrier protein domains at the Lnml nonribosomal peptide synthetase-polyketide synthase interface for leinamycin biosynthesis Organic Letters 2016 18:4288-4291 DOI:10.1021/acs.orglett.6b02033 PMID:27541042 PMCID:PMC5013926
- Huang, T., Chang, C. Y., Lohman, J. R., Rudolf, J. D., Kim, Y., Chang, C., Yang, D., Ma, M., Yan, X., Crnovcic, I., Bigelow, L., Clancy, S., et al. Crystal structure of SgcJ, an NTF2-like superfamily protein involved in biosynthesis of the nine-membered enediyne antitumor antibiotic C-1027 Journal of Antibiotics 2016 69:731-740 DOI:10.1038/ja.2016.88 PMID:27406907 PMCID:PMC5083130
- Chang, C. Y., Lohman, J. R., Cao, H., Tan, K., Rudolf, J. D., Ma, M., Xu, W., Bingman, C. A., Yennamalli, R. M., Bigelow, L., Babnigg, G., Yan, X., et al. Crystal structures of SgcE6 and SgcC, the two-component monooxygenase that catalyzes hydroxylation of a carrier protein-tethered substrate during the biosynthesis of the enediyne antitumor antibiotic C-1027 in streptomyces globisporus Biochemistry 2016 55:5142-5154 DOI:10.1021/acs.biochem.6b00713 PMID:27560143 PMCID:PMC5024704
- Li, W., Li, X., Huang, T., Teng, Q., Crnovcic, I., Rader, C., Shen, B. Engineered production of cancer targeting peptide (CTP)-containing C-1027 in Streptomyces globisporus and biological evaluation Bioorganic & Medicinal Chemistry 2016 24:3887-3892 DOI:10.1016/j.bmc.2016.04.017 PMID:27094150 PMCID:PMC4972678
- Rudolf, J. D., Yan, X., Shen, B. Genome neighborhood network reveals insights into enediyne biosynthesis and facilitates prediction and prioritization for discovery Journal of Industrial Microbiology & Biotechnology 2016 43:261-276 DOI:10.1007/s10295-015-1671-0 PMID:26318027 PMCID:PMC4753101
- Zhang, B., Yang, D., Yan, Y., Pan, G., Xiang, W., Shen, B. Overproduction of lactimidomycin by cross-overexpression of genes encoding Streptomyces antibiotic regulatory proteins Applied Microbiology and Biotechnology 2016 100:2267-2277 DOI:10.1007/s00253-015-7119-7 PMID:26552797 PMCID:PMC4758858
- Estevez, B., Kim, K., Delaney, M. K., Stojanovic-Terpo, A., Shen, B., Ruan, C., Cho, J., Ruggeri, Z. M., Du, X. Signaling-mediated cooperativity between glycoprotein Ib-IX and protease-activated receptors in thrombin-induced platelet activation Blood 2016 127:626-636 DOI:10.1182/blood-2015-04-638387 PMID:26585954 PMCID:PMC4742550
- Yan, X., Ge, H., Huang, T., Hindra, Yang, D., Teng, Q., Crnovcic, I., Li, X., Rudolf, J. D., Lohman, J. R., Gansemans, Y., Zhu, X., et al. Strain prioritization and genome mining for enediyne natural products MBio 2016 7 DOI:10.1128/mBio.02104-16 PMID:27999165 PMCID:PMC5181780
- Rudolf, J. D., Dong, L. B., Cao, H., Hatzos-Skintges, C., Osipiuk, J., Endres, M., Chang, C. Y., Ma, M., Babnigg, G., Joachimiak, A., Phillips, G. N., Shen, B. Structure of the ent-copalyl diphosphate synthase PtmT2 from streptomyces platensis CB00739, a bacterial type II diterpene synthase Journal of the American Chemical Society 2016 138:10905-10915 DOI:10.1021/jacs.6b04317 PMID:27490479 PMCID:PMC5013971
- Smanski, M. J., Zhou, H., Claesen, J., Shen, B., Fischbach, M. A., Voigt, C. A. Synthetic biology to access and expand nature's chemical diversity Nature Reviews Microbiology 2016 14:135-149 DOI:10.1038/nrmicro.2015.24 PMID:26876034 PMCID:PMC5048682
- Shi, J., Pan, J., Liu, L., Yang, D., Lu, S., Zhu, X., Shen, B., Duan, Y., Huang, Y. Titer improvement and pilot-scale production of platensimycin from Streptomyces platensis SB12026 Journal of Industrial Microbiology & Biotechnology 2016 43:1027-1035 DOI:10.1007/s10295-016-1769-z PMID:27126098 PMCID:PMC5056013
- Chen, J. K., Yang, D., Shen, B., Neilan, B. A., Murray, V. Zorbamycin has a different DNA sequence selectivity compared with bleomycin and analogues Bioorganic & Medicinal Chemistry 2016 24:6094-6101 DOI:10.1016/j.bmc.2016.09.072 PMID:27745992
- Rudolf, J. D., Dong, L. B., Huang, T., Shen, B. A genetically amenable platensimycin- and platencin-overproducer as a platform for biosynthetic explorations: a showcase of PtmO4, a long-chain acyl-CoA dehydrogenase Molecular Biosystems 2015 11:2717-2726 DOI:10.1039/c5mb00303b PMID:26055255 PMCID:PMC4573825
- Shen, B. A new golden age of natural products drug discovery Cell 2015 163:1297-1300 DOI:10.1016/j.cell.2015.11.031 PMID:26638061 PMCID:PMC5070666
- Rateb, M. E., Yang, D., Vodanovic-Jankovic, S., Yu, Z., Kron, M. A., Shen, B. Adipostatins A-D from Streptomyces sp 4875 inhibiting Brugia malayi asparaginyl-tRNA synthetase and killing adult Brugia malayi parasites Journal of Antibiotics 2015 68:540-542 DOI:10.1038/ja.2015.22 PMID:25735843
- Ma, M., Rateb, M. E., Teng, Q., Yang, D., Rudolf, J. D., Zhu, X., Huang, Y., Zhao, L. X., Jiang, Y., Li, X., Rader, C., Duan, Y., et al. Angucyclines and angucyclinones from streptomyces sp. CB01913 featuring C-ring cleavage and expansion Journal of Natural Products 2015 78:2471-2480 DOI:10.1021/acs.jnatprod.5b00601 PMID:26335269 PMCID:PMC4845661
- Ma, M., Lohman, J. R., Liu, T., Shen, B. C-S bond cleavage by a polyketide synthase domain Proceedings of the National Academy of Sciences of the United States of America 2015 112:10359-10364 DOI:10.1073/pnas.1508437112 PMID:26240335 PMCID:PMC4547226
- Rudolf, J. D., Bigelow, L., Chang, C., Cuff, M. E., Lohman, J. R., Chang, C. Y., Ma, M., Yang, D., Clancy, S., Babnigg, G., Joachimiak, A., Phillips Jr., G. N., et al. Crystal structure of the zorbamycin-binding protein ZbmA, the primary self-resistance element in Streptomyces flavoviridis ATCC21892 Biochemistry 2015 54:6842-6851 DOI:10.1021/acs.biochem.5b01008 PMID:26512730 PMCID:PMC4809751
- Yang, Z., Cao, S., Martens, C. A., Porcella, S. F., Xie, Z., Ma, M., Shen, B., Moss, B. Deciphering poxvirus gene expression by RNA sequencing and ribosome profiling Journal of Virology 2015 89:6874-6886 DOI:10.1128/jvi.00528-15 PMID:25903347 PMCID:PMC4468498
- Shen, B., Hindra, Yan, X., Huang, T., Ge, H., Yang, D., Teng, Q., Rudolf, J. D., Lohman, J. R. Enediynes: Exploration of microbial genomics to discover new anticancer drug leads Bioorganic & Medicinal Chemistry Letters 2015 25:9-15 DOI:10.1016/j.bmcl.2014.11.019 PMID:25434000 PMCID:PMC4480864
- Rudolf, J. D., Yan, X., Shen, B. Genome neighborhood network reveals insights into enediyne biosynthesis and facilitates prediction and prioritization for discovery Journal of Industrial Microbiology & Biotechnology 2015 DOI:10.1007/s10295-015-1671-0 PMID:26318027 PMCID:PMC4753101
- Huang, S. X., Yun, B. S., Ma, M., Basu, H. S., Church, D. R., Ingenhorst, G., Huang, Y., Yang, D., Lohman, J. R., Tang, G. L., Ju, J., Liu, T., et al. Leinamycin E1 acting as an anticancer prodrug activated by reactive oxygen species Proceedings of the National Academy of Sciences of the United States of America 2015 112:8278-8283 DOI:10.1073/pnas.1506761112 PMID:26056295 PMCID:PMC4500275
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- Lim, S. K., Ju, J. H., Zazopoulos, E., Jiang, H., Seo, J. W., Chen, Y. H., Feng, Z. Y., Rajski, S. R., Farnet, C. M., Shen, B. Iso-migrastatin, migrastatin, and dorrigocin production in streptomyces platensis nrrl 18993 is governed by a single biosynthetic machinery featuring an acyltransferase-less type i polyketide synthase Journal of Biological Chemistry 2009 284:29746-29756 DOI:10.1074/jbc.M109.046805 PMID:19726666 PMCID:PMC2785606
- Ju, J. H., Rajski, S. R., Lim, S. K., Seo, J. W., Peters, N. R., Hoffmann, F. M., Shen, B. Lactimidomycin, iso-migrastatin and related glutarimide-containing 12-membered macrolides are extremely potent inhibitors of cell migration Journal of the American Chemical Society 2009 131:1370-1371 DOI:10.1021/ja808462p PMID:19132897 PMCID:PMC2651819
- Cooke, H. A., Guenther, E. L., Luo, Y. G., Shen, B., Bruner, S. D. Molecular basis of substrate promiscuity for the SAM-dependent O-methyltransferase NcsB1, involved in the biosynthesis of the enediyne antitumor antibiotic neocarzinostatin Biochemistry 2009 48:9590-9598 DOI:10.1021/bi901257q PMID:19702337 PMCID:PMC4500170
- Yu, Y., Duan, L., Zhang, Q., Liao, R. J., Ding, Y., Pan, H. X., Wendt-Pienkowski, E., Tang, G. L., Shen, B., Liu, W. Nosiheptide biosynthesis featuring a unique indole side ring formation on the characteristic thiopeptide framework ACS Chemical Biology 2009 4:855-864 DOI:10.1021/cb900133x PMID:19678698 PMCID:PMC2763056
- Adler, J. T., Cook, M., Luo, Y. G., Pitt, S. C., Ju, J. H., Li, W. L., Shen, B., Kunnimalaiyaan, M., Chen, H. Tautomycetin and tautomycin suppress the growth of medullary thyroid cancer cells via inhibition of glycogen synthase kinase-3 beta Molecular Cancer Therapeutics 2009 8:914-920 DOI:10.1158/1535-7163.mct-08-0712 PMID:19372564 PMCID:PMC2670470
- Pinchot, S. N., Adler, J. T., Luo, Y. G., Ju, J. H., Li, W. L., Shen, B., Kunnimalaiyaan, M., Chen, H. Tautomycin suppresses growth and neuroendocrine hormone markers in carcinoid cells through activation of the raf-1 pathway American Journal of Surgery 2009 197:313-318 DOI:10.1016/j.amjsurg.2008.10.007 PMID:19245907 PMCID:PMC2664746
- Galm, U., Wendt-Pienkowski, E., Wang, L. Y., George, N. P., Oh, T. J., Yi, F., Tao, M. F., Coughlin, J. M., Shen, B. The biosynthetic gene cluster of zorbamycin, a member of the bleomycin family of antitumor antibiotics, from streptomyces flavoviridis atcc 21892 Molecular Biosystems 2009 5:77-90 DOI:10.1039/b814075h PMID:19081934 PMCID:PMC3086045
- Rajski, S. R., Shen, B. The role of evolution in the discovery of new drugs and chemicals ACS Symposium Series 2009 1025:205-232 DOI:10.1021/bk-2009-1025.ch011
- Liao, R. J., Duan, L., Lei, C., Pan, H. X., Ding, Y., Zhang, Q., Chen, D. J., Shen, B., Yu, Y., Liu, W. Thiopeptide biosynthesis featuring ribosomally synthesized precursor peptides and conserved posttranslational modifications Chemistry & Biology 2009 16:141-147 DOI:10.1016/j.chembiol.2009.01.007 PMID:19246004 PMCID:PMC2676563
- Zhang, J., Van Lanen, S. G., Ju, J. H., Liu, W., Dorrestein, P. C., Li, W. L., Kelleher, N. L., Shen, B. A phosphopantetheinylating polyketide synthase producing a linear polyene to initiate enediyne antitumor antibiotic biosynthesis Proceedings of the National Academy of Sciences of the United States of America 2008 105:1460-1465 DOI:10.1073/pnas.0711625105 PMID:18223152 PMCID:PMC2234166
- Van Lanen, S. G., Shen, B. Advances in polyketide synthase structure and function Current Opinion in Drug Discovery & Development 2008 11:186-195 PMID:18283606
- Song, D. F., Coughlin, J., Ju, J. H., Zhou, X. F., Shen, B., Zhao, C. H., Deng, Z. X. Alternative method for site-directed mutagenesis of complex polyketide synthase in streptomyces albus ja3453 Acta Biochimica et Biophysica Sinica 2008 40:319-326 DOI:10.1111/j.1745-7270.2008.00408.x PMID:18401530
- Van Lanen, S. G., Shen, B. Biosynthesis of enediyne antitumor antibiotics Current Topics in Medicinal Chemistry 2008 8:448-459 DOI:10.2174/156802608783955656 PMID:18397168 PMCID:PMC3108100
- Van Lanen, S. G., Lin, S. J., Shen, B. Biosynthesis of the enediyne antitumor antibiotic c-1027 involves a new branching point in chorismate metabolism Proceedings of the National Academy of Sciences of the United States of America 2008 105:494-499 DOI:10.1073/pnas.0708750105 PMID:18182490 PMCID:PMC2206564
- Li, W. L., Ju, J. H., Rajski, S. R., Osada, H., Shen, B. Characterization of the tautomycin biosynthetic gene cluster from streptomyces spiroverticillatus unveiling new insights into dialkylmaleic anhydride and polyketide biosynthesis Journal of Biological Chemistry 2008 283:28607-28617 DOI:10.1074/jbc.M804279200 PMID:18708355 PMCID:PMC2568922
- Lin, S. J., Van Lanen, S. G., Shen, B. Characterization of the two-component, fad-dependent monooxygenase sgcc that requires carrier protein-tethered substrates for the biosynthesis of the enediyne antitumor antibiotic c-1027 Journal of the American Chemical Society 2008 130:6616-6623 DOI:10.1021/ja710601d PMID:18426211
- Montavon, T. J., Christianson, C. V., Festin, G. M., Shen, B., Bruner, S. D. Design and characterization of mechanism-based inhibitors for the tyrosine aminomutase sgtam Bioorganic & Medicinal Chemistry Letters 2008 18:3099-3102 DOI:10.1016/j.bmcl.2007.11.046 PMID:18078753
- Ju, J. H., Rajski, S. R., Lim, S. K., Seo, J. W., Peters, N. R., Hoffmann, F. M., Shen, B. Evaluation of new migrastatin and dorrigocin congeners unveils cell migration inhibitors with dramatically improved potency Bioorganic & Medicinal Chemistry Letters 2008 18:5951-5954 DOI:10.1016/j.bmcl.2008.07.072 PMID:18684620 PMCID:PMC2585373
- Chen, Y., Wendt-Pienkowski, E., Shen, B. Identification and utility of fdmr1 as a streptomyces antibiotic regulatory protein activator for fredericamycin production in streptomyces griseus atcc 49344 and heterologous hosts Journal of Bacteriology 2008 190:5587-5596 DOI:10.1128/jb.00592-08 PMID:18556785 PMCID:PMC2519381
- Chen, Y. H., Luo, Y. G., Ju, J. H., Wendt-Pienkowski, E., Rajski, S. R., Shen, B. Identification of fredericamycin e from streptomyces griseus: Insights into fredericamycin a biosynthesis highlighting carbaspirocycle formation Journal of Natural Products 2008 71:431-437 DOI:10.1021/np070664n PMID:18232659
- Galm, U., Wang, L. Y., Wendt-Pienkowski, E., Yang, R. Y., Liu, W., Tao, M. F., Coughlin, J. M., Shen, B. In vivo manipulation of the bleomycin biosynthetic gene cluster in streptomyces verticillus atcc15003 revealing new insights into its biosynthetic pathway Journal of Biological Chemistry 2008 283:28236-28245 DOI:10.1074/jbc.M804971200 PMID:18697737 PMCID:PMC2568937
- Luo, Y. G., Lin, S. J., Zhang, J., Cooke, H. A., Bruner, S. D., Shen, B. Regiospecific o-methylation of naphthoic acids catalyzed by ncsb1, an o-methyltransferase involved in the biosynthesis of the enediyne antitumor antibiotic neocarzinostatin Journal of Biological Chemistry 2008 283:14694-14702 DOI:10.1074/jbc.M802206200 PMID:18387946 PMCID:PMC2386921
- Jiang, H., Zirkle, R., Metz, J. G., Braun, L., Richter, L., Van Lanen, S. G., Shen, B. The role of tandem acyl carrier protein domains in polyunsaturated fatty acid biosynthesis Journal of the American Chemical Society 2008 130:6336-6337 DOI:10.1021/ja801911t PMID:18444614
- Tang, G. L., Cheng, Y. Q., Shen, B. Chain initiation in the leinamycin-producing hybrid nonribosomal peptide/polyketide synthetase from streptomyces atroolivaceus s-140 - discrete, monofunctional adenylation enzyme and peptidyl carrier protein that directly load d-alanine Journal of Biological Chemistry 2007 282:20273-20282 DOI:10.1074/jbc.M702814200 PMID:17502372
- Cooke, H. A., Zhang, J., Griffin, M. A., Nonaka, K., Van Lanen, S. G., Shen, B., Bruner, S. D. Characterization of NcsB2 as a promiscuous naphthoic acid/coenzyme A ligase integral to the biosynthesis of the enediyne antitumor antibiotic neocarzinostatin Journal of the American Chemical Society 2007 129:7728-7729 DOI:10.1021/ja071886a PMID:17539640
- Van Lanen, S. G., Oh, T. J., Liu, W., Wendt-Pienkowski, E., Shen, B. Characterization of the maduropeptin biosynthetic gene cluster from actinomadura madurae atcc 39144 supporting a unifying paradigm for enediyne biosynthesis Journal of the American Chemical Society 2007 129:13082-13094 DOI:10.1021/ia073275o PMID:17918933
- Kennedy, D. R., Ju, J., Shen, B., Beerman, T. A. Designer enediynes generate DNA breaks, interstrand cross-links, or both, with concomitant changes in the regulation of DNA damage responses Proceedings of the National Academy of Sciences of the United States of America 2007 104:17632-17637 DOI:10.1073/pnas.0708274104 PMID:17978180 PMCID:PMC2077023
- Wang, L. Y., Yun, B. S., George, N. P., Wendt-Pienkowski, E., Galm, U., Oh, T. J., Coughlin, J. M., Zhang, G. D., Tao, M. F., Shen, B. Glycopeptide antitumor antibiotic zorbamycin from streptomyces flavoviridis atcc 21892: Strain improvement and structure elucidation Journal of Natural Products 2007 70:402-406 DOI:10.1021/np060592k PMID:17311457 PMCID:PMC2579990
- Galm, U., Shen, B. Natural product drug discovery: the times have never been better Chemistry & Biology 2007 14:1098-1104 DOI:10.1016/j.chembiol.2007.10.004 PMID:17961822
- Ju, J., Seo, H. W., Her, Y., Lim, S. K., Shen, B. New lactimidomycin congeners shed insight into lactimidomycin biosynthesis in streptomyces amphibiosporus Organic Letters 2007 9:5183-5186 DOI:10.1021/ol702249g PMID:17997563
- Lin, S., Van Lanen, S. G., Shen, B. Regiospecific chlorination of (s)-ss-tyrosyl-s-carrier protein catalyzed by sgcc3 in the biosynthesis of the enediyne antitumor antibiotic c-1027 Journal of the American Chemical Society 2007 129:12432-12438 DOI:10.1021/ja072311g PMID:17887753 PMCID:PMC2631410
- Guan, C. H., Ju, J. J., Borlee, B. R., Williamson, L. L., Shen, B., Raffa, K. F., Handelsman, J. Signal mimics derived from a metagenomic analysis of the gypsy moth gut microbiota Applied and Environmental Microbiology 2007 73:3669-3676 DOI:10.1128/aem.02617-06 PMID:17435000
- Kennedy, D. R., Gawron, L. S., Ju, J. H., Liu, W., Shen, B., Beerman, T. A. Single chemical modifications of the c-1027 enediyne core, a radiomimetic antitumor drug, affect both drug potency and the role of ataxia-telangiectasia mutated in cellular responses to DNA double-strand breaks Cancer Research 2007 67:773-781 DOI:10.1158/0008-5471.can-06-2893 PMID:17234789
- Christianson, C. V., Montavon, T. J., Festin, G. M., Cooke, H. A., Shen, B., Bruner, S. D. The mechanism of mio-based aminomutases in beta-amino acid biosynthesis Journal of the American Chemical Society 2007 129:15744-15745 DOI:10.1021/ja0762689 PMID:18052279
- Christianson, C. V., Montavon, T. J., Van Lanen, S. G., Shen, B., Bruner, S. D. The structure of l-tyrosine 2,3-aminomutase from the c-1027 enediyne antitumor antibiotic biosynthetic pathway Biochemistry 2007 46:7205-7214 DOI:10.1021/bi7003685 PMID:17516659
- Tao, M. F., Wang, L. Y., Wendt-Pienkowski, E., George, N. P., Galm, U., Zhang, G. D., Coughlin, J. M., Shen, B. The tallysomycin biosynthetic gene cluster from streptoalloteichus hindustanus e465-94 atcc 31158 unveiling new insights into the biosynthesis of the bleomycin family of antitumor antibiotics Molecular Biosystems 2007 3:60-74 DOI:10.1039/b615284h PMID:17216057
- Huang, Y., Wendt-Pienkowski, E., Shen, B. A dedicated phosphopantetheinyl transferase for the fredericamycin polyketide synthase from streptomyces griseus Journal of Biological Chemistry 2006 281:29660-29668 DOI:10.1074/jbc.M604895200 PMID:16895912
- Galm, U., Shen, B. Expression of biosynthetic gene clusters in heterologous hosts for natural product production and combinatorial biosynthesis Expert Opinion on Drug Discovery 2006 1:409-437 DOI:10.1517/17460441.1.5.409 PMID:23495943
- Van Lanen, S. G., Shen, B. Microbial genomics for the improvement of natural product discovery Current Opinion in Microbiology 2006 9:252-260 DOI:10.1016/j.mib.2006.04.002 PMID:16651020
- Tang, G. L., Cheng, Y. Q., Shen, B. Polyketide chain skipping mechanism in the biosynthesis of the hybrid nonribosomal peptide-polyketide antitumor antibiotic leinamycin in streptomyces atroolivaceus s-140 Journal of Natural Products 2006 69:387-393 DOI:10.1021/np050467t PMID:16562841 PMCID:PMC2579989
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- Van Lanen, S. G., Lin, S. J., Dorrestein, P. C., Kelleher, N. L., Shen, B. Substrate specificity of the adenylation enzyme sgcc1 involved in the biosynthesis of the enediyne antitumor antibiotic c-1027 Journal of Biological Chemistry 2006 281:29633-29640 DOI:10.1074/jbc.M605887200 PMID:16887797
- Dorrestein, P. C., Van Lanen, S. G., Li, W. L., Zhao, C. H., Deng, Z. X., Shen, B., Kelleher, N. L. The bifunctional glyceryl transferase/phosphatase ozmb belonging to the had superfamily that diverts 1,3-bisphosphoglycerate into polyketide biosynthesis Journal of the American Chemical Society 2006 128:10386-10387 DOI:10.1021/ja0639362 PMID:16895402
- Ju, J. H., Lim, S. K., Jiang, H., Seo, J. W., Her, Y., Shen, B. Thermolysis of isomigrastatin and its congeners via 3,3 -sigmatropic rearrangement: A new route to the synthesis of migrastatin and its analogues Organic Letters 2006 8:5865-5868 DOI:10.1021/01062470p PMID:17134292
- Li, W. L., Ju, J. H., Osada, H., Shen, B. Utilization of the methoxymalonyl-acyl carrier protein biosynthesis locus for cloning of the tautomycin biosynthetic gene cluster from streptomyces spiroverticillatus Journal of Bacteriology 2006 188:4148-4152 DOI:10.1128/jb.00172-06 PMID:16707708
- Zhao, C. H., Ju, J. H., Christenson, S. D., Smith, W. C., Song, D. F., Zhou, X. F., Shen, B., Deng, Z. X. Utilization of the methoxymalonyl-acyl carrier protein biosynthesis locus for cloning the oxazolomycin biosynthetic gene cluster from streptomyces albus ja3453 Journal of Bacteriology 2006 188:4142-4147 DOI:10.1128/jb.00173-06 PMID:16707707
- Galm, U., Hager, M. H., Van Lanen, S. G., Ju, J. H., Thorson, J. S., Shen, B. Antitumor antibiotics: Bleomycin, endiynes, and mitomycin Chemical Reviews 2005 105:739-758 DOI:10.1021/cr030117g PMID:15700963
- Van Lanen, S. G., Dorrestein, P. C., Christenson, S. D., Liu, W., Ju, J. H., Kelleher, N. L., Shen, B. Biosynthesis of the beta-amino acid moiety of the enediyne antitumor antibiotic c-1027 featuring beta-amino acyl-s-carrier protein intermediates Journal of the American Chemical Society 2005 127:11594-11595 DOI:10.1021/ja052871k PMID:16104723
- Wendt-Pienkowski, E., Huang, Y., Zhang, J., Li, B. S., Jiang, H., Kwon, H. J., Hutchinson, C. R., Shen, B. Cloning, sequencing, analysis, and heterologous expression of the fredericamycin biosynthetic gene cluster from streptomyces griseus Journal of the American Chemical Society 2005 127:16442-16452 DOI:10.1021/ja054376u PMID:16305230
- Ju, J. H., Lim, S. K., Jiang, H., Seo, J. W., Shen, B. Iso-migrastatin congeners from streptomyces platensis and generation of a glutarimide polyketide library featuring the dorrigocin, lactimidomycin, migrastatin, and nk30424 scaffolds Journal of the American Chemical Society 2005 127:11930-11931 DOI:10.1021/ja053118u PMID:16117518
- Ju, J. H., Lim, S. K., Jiang, H., Shen, B. Migrastatin and dorrigocins are shunt metabolites of iso-migrastatin Journal of the American Chemical Society 2005 127:1622-1623 DOI:10.1021/ja043808i PMID:15700980
- Liu, W., Nonaka, K., Nie, L. P., Zhang, J., Christenson, S. D., Bae, J., Van Lanen, S. G., Zazopoulos, E., Farnet, C. M., Yang, C. F., Shen, B. The neocarzinostatin biosynthetic gene cluster from streptomyces carzinostaticus atcc 15944 involving two iterative type i polyketide synthases Chemistry & Biology 2005 12:293-302 DOI:10.1016/j.chembiol.2004.12.013 PMID:15797213
- Shen, B. Accessing natural products by combinatorial biosynthesis Science's STKE : Signal Transduction Knowledge Environment 2004 2004:pe14 DOI:10.1126/stke.2252004pe14 PMID:15039491
- Murrell, J. M., Liu, W., Shen, B. Biochemical characterization of the sgca1 alpha-d-glucopyranosyl-1-phosphate thymidylyltransferase from the enediyne antitumor antibiotic c-1027 biosynthetic pathway and overexpression of sgca1 in streptomyces globisporus to improve c-1027 production Journal of Natural Products 2004 67:206-213 DOI:10.1021/np0340403 PMID:14987060
- Ju, J. H., Ozanick, S. G., Shen, B., Thomas, M. G. Conversion of (2s)-arginine to (2s,3r)-capreomycidine by vioc and viod from the viomycin biosynthetic pathway of streptomyces sp strain atcc11861 ChemBioChem 2004 5:1281-1285 DOI:10.1002/cbic.200400136 PMID:15368582
- Tang, G. L., Cheng, Y. Q., Shen, B. Leinamycin biosynthesis revealing unprecedented architectural complexity for a hybrid polyketide synthase and nonribosomal peptide synthetase Chemistry & Biology 2004 11:33-45 DOI:10.1016/j.chembiol.2003.12.014 PMID:15112993
- Chapados, B. R., Hosfield, D. J., Han, S., Qiu, J. Z., Yelent, B., Shen, B. H., Tainer, J. A. Structural basis for FEN-1 substrate specificity and PCNA-mediated activation in DNA replication and repair Cell 2004 116:39-50 DOI:10.1016/s0092-8674(03)01036-5 PMID:14718165
- Zazopoulos, E., Huang, K. X., Staffa, A., Liu, W., Bachmann, B. O., Nonaka, K., Ahlert, J., Thorson, J. S., Shen, B., Farnet, C. M. A genomics-guided approach for discovering and expressing cryptic metabolic pathways Nature Biotechnology 2003 21:187-190 DOI:10.1038/nbt784 PMID:12536216
- Christenson, S. D., Liu, W., Toney, M. D., Shen, B. A novel 4-methylideneimidazole-5-one-containing tyrosine aminomutase in enediyne antitumor antibiotic c-1027 biosynthesis Journal of the American Chemical Society 2003 125:6062-6063 DOI:10.1021/ja034609m PMID:12785829
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- Schneider, T. L., Shen, B., Walsh, C. T. Oxidase domains in epothilone and bleomycin biosynthesis: Thiazoline to thiazole oxidation during chain elongation Biochemistry 2003 42:9722-9730 DOI:10.1021/bi034792w PMID:12911314
- Shen, B. Polyketide biosynthesis beyond the type i, ii and iii polyketide synthase paradigms Current Opinion in Chemical Biology 2003 7:285-295 DOI:10.1016/s1367-5931(03)00020-6 PMID:12714063
- Liu, W., Ahlert, J., Gao, Q. J., Wendt-Pienkowski, E., Shen, B., Thorson, J. S. Rapid pcr amplification of minimal enediyne polyketide synthase cassettes leads to a predictive familial classification model Proceedings of the National Academy of Sciences of the United States of America 2003 100:11959-11963 DOI:10.1073/pnas.2034291100 PMID:14528002 PMCID:PMC218695
- Cheng, Y. Q., Tang, G. L., Shen, B. Type i polyketide synthase requiring a discrete acyltransferase for polyketide biosynthesis Proceedings of the National Academy of Sciences of the United States of America 2003 100:3149-3154 DOI:10.1073/pnas.0537286100 PMID:12598647 PMCID:PMC152261
- Liu, W., Christenson, S. D., Standage, S., Shen, B. Biosynthesis of the enediyne antitumor antibiotic c-1027 Science 2002 297:1170-1173 DOI:10.1126/science.1072110 PMID:12183628
- Kwon, H. J., Smith, W. C., Scharon, A. J., Hwang, S. H., Kurth, M. J., Shen, B. C-o bond formation by polyketide synthases Science 2002 297:1327-1330 DOI:10.1126/science.1073175 PMID:12193782
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- Cheng, Y. Q., Tang, G. L., Shen, B. Identification and localization of the gene cluster encoding biosynthesis of the antitumor macrolactam leinamycin in streptomyces atroolivaceus s-140 Journal of Bacteriology 2002 184:7013-7024 DOI:10.1128/jb.184.24.7013-7024.2002 PMID:12446651
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- Chai, Q., Qiu, J., Chapados, B. R., Shen, B. Archaeoglobus fulgidus RNase HII in DNA replication: enzymological functions and activity regulation via metal cofactors Biochemical and Biophysical Research Communications 2001 286:1073-1081 DOI:10.1006/bbrc.2001.5523 PMID:11527410
- Du, L., Shen, B. Biosynthesis of hybrid peptide-polyketide natural products Current Opinion in Drug Discovery & Development 2001 4:215-228 PMID:11378961
- Sanchez, C., Du, L. C., Edwards, D. J., Toney, M. D., Shen, B. Cloning and characterization of a phosphopantetheinyl transferase from streptomyces verticillus atcc15003, the producer of the hybrid peptide-polyketide antitumor drug bleomycin Chemistry & Biology 2001 8:725-738 DOI:10.1016/s1074-5521(01)00047-3 PMID:11451672
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- Du, L. H., Sanchez, C., Shen, B. Hybrid peptide-polyketide natural products: Biosynthesis and prospects toward engineering novel molecules Metabolic Engineering 2001 3:78-95 DOI:10.1006/mben.2000.0171 PMID:11162234
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- Du, L. C., Chen, M., Sanchez, C., Shen, B. An oxidation domain in the blmiii non-ribosomal peptide synthetase probably catalyzing thiazole formation in the biosynthesis of the anti-tumor drug bleomycin in streptomyces verticillus atcc15003 FEMS Microbiology Letters 2000 189:171-175 DOI:10.1111/j.1574-6968.2000.tb09225.x PMID:10930733
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- Shen, B., Du, L. C., Sanchez, C., Chen, M., Edwards, D. J. Bleomycin biosynthesis in streptomyces verticillus atcc15003: A model of hybrid peptide and polyketide biosynthesis Bioorganic Chemistry 1999 27:155-171 DOI:10.1006/bioo.1998.1131
- Thorson, J. S., Shen, B., Whitwam, R. E., Liu, W., Li, Y., Ahlert, J. Enediyne biosynthesis and self-resistance: A progress report Bioorganic Chemistry 1999 27:172-188 DOI:10.1006/bioo.1998.1122
- Du, L. C., Shen, B. Identification and characterization of a type ii peptidyl carrier protein from the bleomycin producer streptomyces verticillus atcc 15003 Chemistry & Biology 1999 6:507-517 DOI:10.1016/s1074-5521(99)80083-0 PMID:10421758
- Shen, B., Hutchinson, C. R. Deciphering the mechanism for the assembly of aromatic polyketides by a bacterial polyketide synthase Proceedings of the National Academy of Sciences of the United States of America 1996 93:6600-6604 DOI:10.1073/pnas.93.13.6600 PMID:8692863
- Summers, R. G., Ali, A., Shen, B., Wessel, W. A., Hutchinson, C. R. Malonyl-coenzyme a-acyl carrier protein acyltransferase of streptomyces-glaucescens - a possible link between fatty-acid and polyketide biosynthesis Biochemistry 1995 34:9389-9402 DOI:10.1021/bi00029a015 PMID:7626609
- Shen, B., Summers, R. G., Wendtpienkowski, E., Hutchinson, C. R. The streptomyces-glaucescens tcmkl polyketide synthase and tcmn polyketide cyclase genes govern the size and shape of aromatic polyketides Journal of the American Chemical Society 1995 117:6811-6821 DOI:10.1021/ja00131a002
- Shen, B., Hutchinson, C. R. Triple hydroxylation of tetracenomycin-a2 to tetracenomycin-c in streptomyces-glaucescens - overexpression of the tcmg gene in streptomyces-lividans and characterization of the tetracenomycin-a2 oxygenase Journal of Biological Chemistry 1994 269:30726-30733 PMID:7982994
- Shen, B., Hutchinson, C. R. Enzymatic-synthesis of a bacterial polyketide from acetyl and malonyl coenzyme-a Science 1993 262:1535-1540 DOI:10.1126/science.8248801 PMID:8248801
- Shen, B., Nakayama, H., Hutchinson, C. R. Isolation and structural elucidation of tetracenomycin-f2 and tetracenomycin-f1 - early intermediates in the biosynthesis of tetracenomycin-c in streptomyces-glaucescens Journal of Natural Products 1993 56:1288-1293 DOI:10.1021/np50098a013 PMID:8229013
- Shen, B., Hutchinson, C. R. Tetracenomycin f1-monooxygenase - oxidation of a naphthacenone to a naphthacenequinone in the biosynthesis of tetracenomycin-c in streptomyces-glaucescens Biochemistry 1993 32:6656-6663 DOI:10.1021/bi00077a019 PMID:8329392
- Shen, B., Hutchinson, C. R. Tetracenomycin-f2 cyclase - intramolecular aldol condensation in the biosynthesis of tetracenomycin-c in streptomyces-glaucescens Biochemistry 1993 32:11149-11154 DOI:10.1021/bi00092a026 PMID:8218177
- Hutchinson, C. R., Decker, H., Guilfoile, P., Shen, B., Summers, R., Wendt-Pienkowski, E., Wessel, B. Polyketide synthases: enzyme complexes and multifunctional proteins directing the biosynthesis of bacterial metabolites from fatty acids Environmental Science Research 1992 44:3-10
- Shen, B., Summers, R. G., Gramajo, H., Bibb, M. J., Hutchinson, C. R. Purification and characterization of the acyl carrier protein of the streptomyces-glaucescens tetracenomycin-c polyketide synthase Journal of Bacteriology 1992 174:3818-3821 PMID:1592832 PMCID:PMC206074
- Gould, S. J., Shen, B. Epoxyquinones from 2,5-dihydroxyacetanilide - opposite facial specificity in the epoxidation by enzymes from streptomyces ll-c10037 and streptomyces mpp 3051 Journal of the American Chemical Society 1991 113:684-686 DOI:10.1021/ja00002a052
- Shen, B., Gould, S. J. Opposite facial specificity for 2 hydroquinone epoxidases - (3-si,4-re)-2,5-dihydroxyacetanilide epoxidase from streptomyces ll-c10037 and (3-re,4-si)-2,5-dihydroxyacetanilide epoxidase from streptomyces mpp-3051 Biochemistry 1991 30:8936-8944 DOI:10.1021/bi00101a004 PMID:1892811
- Shen, B., Whittle, Y. G., Gould, S. J., Keszler, D. A. Structure and absolute stereochemistry of the epoxyquinol ll-c10037-alpha and related metabolites from streptomyces ll-c10037 Journal of Organic Chemistry 1990 55:4422-4426 DOI:10.1021/jo00301a039
- Gould, S. J., Shen, B., Whittle, Y. G. Biosynthesis of antibiotic ll-c10037-alpha - the steps beyond 3-hydroxyanthranilic acid Journal of the American Chemical Society 1989 111:7932-7938 DOI:10.1021/ja00202a039
- Liu, C., Chen, Q., Yu, Z., Shen, B. Mordenite-catalyzed three-phase hydration of alpha-pinene: product distribution, kinetics and reaction mechanism Acta Chimica Sinica 1986 44:545-550

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