recent publications
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academic article
- Qi, J. P., Tsuji, K., Hymel, D., Burke, T. R., Hudecek, M., Rader, C., Peng, H. Chemically Programmable and Switchable CAR-T Therapy Angewandte Chemie-International Edition 2020 DOI:10.1002/anie.202005432 PMID:32329959
- Goydel, R. S., Weber, J., Peng, H. Y., Qi, J. P., Soden, J., Freeth, J., Park, H., Rader, C. Affinity maturation, humanization, and co-crystallization of a rabbit anti-human ROR2 monoclonal antibody for therapeutic applications Journal of Biological Chemistry 2020 295:5995-6006 DOI:10.1074/jbc.RA120.012791 PMID:32193207 PMCID:PMC7196640
- Hwang, D., Rader, C. Site-Specific Antibody-Drug Conjugates in Triple Variable Domain Fab Format Biomolecules 2020 10 DOI:10.3390/biom10050764 PMID:32422893 PMCID:PMC7278019
- Adhikari, A., Teijaro, C. N., Yan, X. H., Chang, C. Y., Gui, C., Liu, Y. C., Crnovcic, I., Yang, D., Annaval, T., Rader, C., Shen, B. Characterization of TnmH as an O-Methyltransferase Revealing Insights into Tiancimycin Biosynthesis and Enabling a Biocatalytic Strategy To Prepare Antibody-Tiancimycin Conjugates Journal of Medicinal Chemistry 2020 63:8432-8441 DOI:10.1021/acs.jmedchem.0c00799 PMID:32658465 PMCID:PMC7461649
- Nanna, A. R., Kel'in, A. V., Theile, C., Pierson, J. M., Voo, Z. X., Garg, A., Nair, J. K., Maier, M. A., Fitzgerald, K., Rader, C. Generation and validation of structurally defined antibody-siRNA conjugates Nucleic Acids Research 2020 48:5281-5293 DOI:10.1093/nar/gkaa286 PMID:32347936 PMCID:PMC7261152
- Hwang, D., Tsuji, K., Park, H., Burke, T. R., Rader, C. Site-specific lysine arylation as an alternative bioconjugation strategy for chemically programmed antibodies and antibody-drug conjugates Bioconjugate Chemistry 2019 30:2889-2896 DOI:10.1021/acs.bioconjchem.9b00609 PMID:31675216
- Hwang, D.*, Nilchan, N.*, Nanna, A. R., Li, X., Cameron, M. D., Roush, W. R., Park, H., Rader, C. Site-selective antibody functionalization via orthogonally reactive arginine and lysine residues Cell Chemical Biology 2019 26:1229-1239 DOI:10.1016/j.chembiol.2019.05.010 PMID:31231031 PMCID:PMC6754284 (* = equal contribution)
- Rader, C., Wiestner, A. Six-packed antibodies punch better Haematologica 2019 104:1696-1699 DOI:10.3324/haematol.2019.224196 PMID:31473607 PMCID:PMC6717577
- Qi, J., Hymel, D., Nelson, C. G., Burke Jr., T. R., Rader, C. Conventional and chemically programmed asymmetric bispecific antibodies targeting folate receptor 1 Frontiers in Immunology 2019 10 DOI:10.3389/fimmu.2019.01994 PMCID:PMC6712926
- Chiang, C. L., Goswami, S., Frissora, F. W., Xie, Z., Yan, P. S., Bundschuh, R., Walker, L. A., Huang, X. M., Mani, R., Mo, X. K. M., Baskar, S., Rader, C., et al. ROR1-targeted delivery of miR-29b induces cell cycle arrest and therapeutic benefit in vivo in a CLL mouse model Blood 2019 134:432-444 DOI:10.1182/blood.2018882290 PMID:31151986
- Srivastava, S., Salter, A. I., Liggitt, D., Yechan-Gunja, S., Sarvothama, M., Cooper, K., Smythe, K. S., Dudakov, J. A., Pierce, R. H., Rader, C., Ridde, S. R. Logic-gated ROR1 chimeric antigen receptor expression rescues T cell-mediated toxicity to normal tissues and enables selective tumor targeting Cancer Cell 2019 35:489-503 DOI:10.1016/j.ccell.2019.02.003 PMID:30889382 PMCID:PMC6450658
- Mage, R. G., Esteves, P. J., Rader, C. Rabbit models of human diseases for diagnostics and therapeutics development Developmental and Comparative Immunology 2019 92:99-104 DOI:10.1016/j.dci.2018.10.003 PMID:30339876 PMCID:PMC6364550
- Rydzek, J., Nerreter, T., Peng, H., Jutz, S., Leitner, J., Steinberger, P., Einsele, H., Rader, C., Hudecek, M. Chimeric antigen receptor library screening using a novel NF-κB/NFAT reporter cell platform Molecular Therapy 2019 27:287-299 DOI:10.1016/j.ymthe.2018.11.015 PMID:30573301 PMCID:PMC6369451
- Qi, J., Chen, S. S., Chiorazzi, N., Rader, C. An IgG1-like bispecific antibody targeting CD52 and CD20 for the treatment of B-cell malignancies Methods 2019 154:70-76 DOI:10.1016/j.ymeth.2018.08.008 PMID:30145356
- Wilson, H. D., Li, X., Peng, H., Rader, C. A sortase a programmable phage display format for improved panning of Fab antibody libraries Journal of Molecular Biology 2018 430:4387-4400 DOI:10.1016/j.jmb.2018.09.003 PMID:30213726 PMCID:PMC6186506
- Yan, X., Chen, J. J., Adhikari, A., Teijaro, C. N., Ge, H., Crnovcic, I., Chang, C. Y., Annaval, T., Yang, D., Rader, C., Shen, B. Comparative studies of the biosynthetic gene clusters for anthraquinone-fused enediynes shedding light into the tailoring steps of tiancimycin biosynthesis Organic Letters 2018 20:5918-5921 DOI:10.1021/acs.orglett.8b02584 PMID:30212211
- Chang, J., Peng, H., Shaffer, B. C., Baskar, S., Wecken, I. C., Cyr, M. G., Martinez, G. J., Soden, J., Freeth, J., Wiestner, A., Rader, C. Siglec-6 on chronic lymphocytic leukemia cells is a target for post-allogeneic hematopoietic stem cell transplantation antibodies Cancer Immunology Research 2018 6:1008-1013 DOI:10.1158/2326-6066.cir-18-0102 PMID:29980538 PMCID:PMC6125214
- Robinson, H. R., Qi, J., Cook, E. M., Nichols, C., Dadashian, E. L., Underbayev, C., Herman, S. E. M., Saba, N. S., Keyvanfar, K., Sun, C., Ahn, I. E., Baskar, S., et al. A CD19/CD3 bispecific antibody for effective immunotherapy of chronic lymphocytic leukemia in the ibrutinib era Blood 2018 132:521-532 DOI:10.1182/blood-2018-02-830992 PMID:29743179 PMCID:PMC6073325
- Qi, J., Li, X., Peng, H., Cook, E. M., Dadashian, E. L., Wiestner, A., Park, H., Rader, C. Potent and selective antitumor activity of a T cell-engaging bispecific antibody targeting a membrane-proximal epitope of ROR1 Proceedings of the National Academy of Sciences of the United States of America 2018 115:E5467-E5476 DOI:10.1073/pnas.1719905115 PMID:29844189
- Yan, X., Chen, J. J., Adhikari, A., Yang, D., Crnovcic, I., Wang, N., Chang, C. Y., Rader, C., Shen, B. Genome mining of micromonospora yangpuensis DSM 45577 as a producer of an anthraquinone-fused enediyne Organic Letters 2017 19:6192-6195 DOI:10.1021/acs.orglett.7b03120 PMID:29086572
- Nanna, A. R., Li, X., Walseng, E., Pedzisa, L., Goydel, R. S., Hymel, D., Burke, T. R., Roush, W. R., Rader, C. Harnessing a catalytic lysine residue for the one-step preparation of homogeneous antibody-drug conjugates Nature Communications 2017 8 DOI:10.1038/s41467-017-01257-1 PMID:29062027 PMCID:PMC5653646
- Zhuang, X., Watts, N. R., Palmer, I. W., Kaufman, J. D., Dearborn, A. D., Trenbeath, J. L., Eren, E., Steven, A. C., Rader, C., Wingfield, P. T. Chimeric rabbit/human Fab antibodies against the hepatitis Be-antigen and their potential applications in assays, characterization, and therapy Journal of Biological Chemistry 2017 292:16760-16772 DOI:10.1074/jbc.M117.802272 PMID:28842495 PMCID:PMC5633136
- Peng, H., Nerreter, T., Chang, J., Qi, J., Li, X., Karunadharma, P., Martinez, G. J., Fallahi, M., Soden, J., Freeth, J., Beerli, R. R., Grawunder, U., et al. Mining naive rabbit antibody repertoires by phage display for monoclonal antibodies of therapeutic utility Journal of Molecular Biology 2017 429:2954-2973 DOI:10.1016/j.jmb.2017.08.003 PMID:28818634 PMCID:PMC5610937
- Li, X., Nelson, C. G., Nair, R. R., Hazlehurst, L., Moroni, T., Martinez-Acedo, P., Nanna, A. R., Hymel, D., Burke, T. R., Rader, C. Stable and potent selenomab-drug conjugates Cell Chemical Biology 2017 24:433-442 DOI:10.1016/j.chembiol.2017.02.012 PMID:28330604 PMCID:PMC5400723
- Moreadith, R. W., Viegas, T. X., Bentley, M. D., Harris, J. M., Fang, Z., Yoon, K., Dizman, B., Weimer, R., Rae, B. P., Li, X., Rader, C., Standaert, D., et al. Clinical development of a poly(2-oxazoline) (POZ) polymer therapeutic for the treatment of Parkinson's disease - Proof of concept of POZ as a versatile polymer platform for drug development in multiple therapeutic indications European Polymer Journal 2017 88:524-552 DOI:10.1016/j.eurpolymj.2016.09.052
- Weber, J., Peng, H., Rader, C. From rabbit antibody repertoires to rabbit monoclonal antibodies Experimental and Molecular Medicine 2017 49 DOI:10.1038/emm.2017.23 PMID:28336958 PMCID:PMC5382564
- Yan, X., Ge, H., Huang, T., Hindra, Yang, D., Teng, Q., Crnovcic, I., Li, X., Rudolf, J. D., Lohman, J. R., Gansemans, Y., Zhu, X., et al. Strain prioritization and genome mining for enediyne natural products MBio 2016 7 DOI:10.1128/mBio.02104-16 PMID:27999165 PMCID:PMC5181780
- Patterson, J. T., Wilson, H. D., Asano, S., Nilchan, N., Fuller, R. P., Roush, W. R., Rader, C., Barbas III, C. F. Human serum albumin domain I fusion protein for antibody conjugation Bioconjugate Chemistry 2016 27:2271-2275 DOI:10.1021/acs.bioconjchem.6b00432 PMID:27666414
- Walseng, E., Nelson, C. G., Qi, J., Nanna, A. R., Roush, W. R., Goswami, R. K., Sinha, S. C., Burke Jr., T. R., Rader, C. Chemically programmed bispecific antibodies in diabody format Journal of Biological Chemistry 2016 291:19661-19673 DOI:10.1074/jbc.M116.745588 PMID:27445334 PMCID:PMC5016699
- Li, W., Li, X., Huang, T., Teng, Q., Crnovcic, I., Rader, C., Shen, B. Engineered production of cancer targeting peptide (CTP)-containing C-1027 in Streptomyces globisporus and biological evaluation Bioorganic & Medicinal Chemistry 2016 24:3887-3892 DOI:10.1016/j.bmc.2016.04.017 PMID:27094150 PMCID:PMC4972678
- Gnaim, S., Scomparin, A., Li, X., Baran, P. S., Rader, C., Satchi-Fainaro, R., Shabat, D. Tagging the untaggable: a difluoroalkyl-sulfinate ketone-based reagent for direct C-H functionalization of bioactive heteroarenes Bioconjugate Chemistry 2016 27:1965-1971 DOI:10.1021/acs.bioconjchem.6b00382 PMID:27494153
- Pedzisa, L., Li, X., Rader, C., Roush, W. R. Assessment of reagents for selenocysteine conjugation and the stability of selenocysteine adducts Organic & Biomolecular Chemistry 2016 14:5141-5147 DOI:10.1039/c6ob00775a PMID:27184239 PMCID:PMC4887430
- Sarkar, M., Liu, Y., Qi, J., Peng, H., Morimoto, J., Rader, C., Chiorazzi, N., Kodadek, T. Targeting stereotyped B cell receptors from chronic lymphocytic leukemia patients with synthetic antigen surrogates Journal of Biological Chemistry 2016 291:7558-7570 DOI:10.1074/jbc.M115.701656 PMID:26851280 PMCID:PMC4817184
- Li, X., Patterson, J. T., Sarkar, M., Pedzisa, L., Kodadek, T., Roush, W. R., Rader, C. Site-specific dual antibody conjugation via engineered cysteine and selenocysteine residues Bioconjugate Chemistry 2015 26:2243-2248 DOI:10.1021/acs.bioconjchem.5600244 PMID:26161903
- Ma, M., Rateb, M. E., Teng, Q., Yang, D., Rudolf, J. D., Zhu, X., Huang, Y., Zhao, L. X., Jiang, Y., Li, X., Rader, C., Duan, Y., et al. Angucyclines and angucyclinones from streptomyces sp. CB01913 featuring C-ring cleavage and expansion Journal of Natural Products 2015 78:2471-2480 DOI:10.1021/acs.jnatprod.5b00601 PMID:26335269 PMCID:PMC4845661
- Mani, R., Chiang, C. L., Frissora, F. W., Yan, R., Mo, X., Baskar, S., Rader, C., Klisovic, R., Phelps, M. A., Chen, C. S., Lee, R. J., Byrd, J. C., et al. ROR1-targeted delivery of OSU-2S, a nonimmunosuppressive FTY720 derivative, exerts potent cytotoxicity in mantle-cell lymphoma in vitro and in vivo Experimental Hematology 2015 43:770-774 DOI:10.1016/j.exphem.2015.04.008 PMID:25937048
- Huang, X., Park, H., Greene, J., Pao, J., Mulvey, E., Zhou, S. X., Albert, C. M., Moy, F., Sachdev, D., Yee, D., Rader, C., Hamby, C. V., et al. IGF1R-and ROR1-specific CAR T cells as a potential therapy for high risk sarcomas PLoS One 2015 10:e0133152 DOI:10.1371/journal.pone.0133152 PMID:26173023 PMCID:PMC4501840
- Chinthalapudi, K., Patil, D. N., Rangarajan, E. S., Rader, C., Izard, T. Lipid-directed vinculin dimerization Biochemistry 2015 54:2758-2768 DOI:10.1021/acs.biochem.5b00015 PMID:25880222
- Rader, C. How to minimalize antibodies Nature 2015 518:38-39 PMID:25652990
- Berger, C., Sommermeyer, D., Hudecek, M., Berger, M., Balakrishnan, A., Paszkiewicz, P. J., Kosasih, P. L., Rader, C., Riddell, S. R. Safety of targeting ROR1 in primates with chimeric antigen receptor-modified T cells Cancer Immunology Research 2015 3:206-216 DOI:10.1158/2326-6066.cir-14-0163 PMID:25355068 PMCID:PMC4324006
- Hudecek, M., Sommermeyer, D., Kosasih, P. L., Silva-Benedict, A., Liu, L., Rader, C., Jensen, M. C., Riddell, S. R. The nonsignaling extracellular spacer domain of chimeric antigen receptors is decisive for in vivo antitumor activity Cancer Immunology Research 2015 3:125-135 DOI:10.1158/2326-6066.cir-14-0127 PMID:25212991
- Mani, R., Mao, Y., Frissora, F. W., Chiang, C. L., Wang, J., Zhao, Y., Wu, Y., Yu, B., Yan, R., Mo, X., Yu, L., Flynn, J., et al. Tumor antigen ROR1 targeted drug delivery mediated selective leukemic but not normal B-cell cytotoxicity in chronic lymphocytic leukemia Leukemia 2015 29:346-355 DOI:10.1038/leu.2014.199 PMID:24947019 PMCID:PMC4272672
- Sarkar, M., Liu, Y., Morimoto, J., Peng, H., Aquino, C., Rader, C., Chiorazzi, N., Kodadek, T. Recognition of antigen-specific B-cell receptors from chronic lymphocytic leukemia patients by synthetic antigen surrogates Chemistry & Biology 2014 21:1670-1679 DOI:10.1016/j.chembiol.2014.10.010 PMID:25467125 PMCID:PMC4272629
- Vire, B., Skarzynski, M., Thomas, J. D., Nelson, C. G., David, A., Aue, G., Burke Jr., T. R., Rader, C., Wiestner, A. Harnessing the fcμ receptor for potent and selective cytotoxic therapy of chronic lymphocytic leukemia Cancer Research 2014 74:7510-7520 DOI:10.1158/0008-5472.can-14-2030 PMID:25344228 PMCID:PMC4268434
- Rader, C., Segal, D. J., Shabat, D. Carlos F. Barbas III (1964-2014): Visionary at the interface of chemistry and biology ACS Chemical Biology 2014 9:1645-1646 DOI:10.1021/cb5005993 PMID:25123302
- Patterson, J. T., Asano, S., Li, X., Rader, C., Barbas III, C. F. Improving the serum stability of site-specific antibody conjugates with sulfone linkers Bioconjugate Chemistry 2014 25:1402-1407 DOI:10.1021/bc500276m PMID:25099687 PMCID:PMC4140540
- Gronwall, C., Charles, E. D., Dustin, L. B., Rader, C., Silverman, G. J. Selection of apoptotic cell specific human antibodies from adult bone marrow PLoS One 2014 9:e95999 DOI:10.1371/journal.pone.0095999 PMID:24760047 PMCID:PMC3997490
- Rader, Christoph Chemically programmed antibodies Trends in Biotechnology 2014 32:186-197 DOI:10.1016/j.tibtech.2014.02.003 PMID:24630478 PMCID:PMC3978777
- Li, X., Yang, J., Rader, C. Antibody conjugation via one and two C-terminal selenocysteines Methods 2014 65:133-138 DOI:10.1016/j.ymeth.2013.05.023 PMID:23756202 PMCID:PMC3871924
- Hudecek, M., Lupo-Stanghellini, M. T., Kosasih, P. L., Sommermeyer, D., Jensen, M. C., Rader, C., Riddell, S. R. Receptor affinity and extracellular domain modifications affect tumor recognition by ROR1-specific chimeric antigen receptor T cells Clinical Cancer Research 2013 19:3153-3164 DOI:10.1158/1078-0432.ccr-13-0330 PMID:23620405
- Dave, H., Anver, M. R., Butcher, D. O., Brown, P., Khan, J., Wayne, A. S., Baskar, S., Rader, C. Restricted cell surface expression of receptor tyrosine kinase ROR1 in pediatric B-lineage acute lymphoblastic leukemia suggests targetability with therapeutic monoclonal antibodies PLoS One 2012 7:e52655 DOI:10.1371/journal.pone.0052655 PMID:23285131 PMCID:PMC3527582
- Thomas, J. D., Cui, H. T., North, P. J., Hofer, T., Rader, C., Burke, T. R. Application of strain-promoted azide-alkyne cycloaddition and tetrazine ligation to targeted Fc-drug conjugates Bioconjugate Chemistry 2012 23:2007-2013 DOI:10.1021/bc300052u PMID:22988967 PMCID:PMC3474869
- Cui, H. T., Thomas, J. D., Burke, T. R., Rader, C. Chemically programmed bispecific antibodies that recruit and activate T cells Journal of Biological Chemistry 2012 287:28206-28214 DOI:10.1074/jbc.M112.384594 PMID:22761439 PMCID:PMC3436515
- Baskar, S., Wiestner, A., Wilson, W. H., Pastan, I., Rader, C. Targeting malignant B cells with an immunotoxin against ROR1 MAbs 2012 4:349-361 DOI:10.4161/mabs.19870 PMID:22531447 PMCID:PMC3355487
- Yang, J., Rader, C. Cloning, expression, and purification of monoclonal antibodies in scFv-Fc format Methods in Molecular Biology 2012 901:209-232 DOI:10.1007/978-1-61779-931-0_14 PMID:22723104
- Rader, C. Generation of human Fab libraries for phage display Methods in Molecular Biology 2012 901:53-79 DOI:10.1007/978-1-61779-931-0_4 PMID:22723094
- Rader, C. Selection of human Fab libraries by phage display Methods in Molecular Biology 2012 901:81-99 DOI:10.1007/978-1-61779-931-0_5 PMID:22723095
- Yang, J. H., Baskar, S., Kwong, K. Y., Kennedy, M. G., Wiestner, A., Rader, C. Therapeutic potential and challenges of targeting receptor tyrosine kinase ROR1 with monoclonal antibodies in B-cell malignancies PLoS One 2011 6:e21018 DOI:10.1371/journal.pone.0021018 PMID:21698301 PMCID:PMC3115963
- Bagnara, D., Kaufman, M. S., Calissano, C., Marsilio, S., Patten, P. E. M., Simone, R., Chum, P., Yan, X. J., Allen, S. L., Kolitz, J. E., Baskar, S., Rader, C., et al. A novel adoptive transfer model of chronic lymphocytic leukemia suggests a key role for T lymphocytes in the disease Blood 2011 117:5463-5472 DOI:10.1182/blood-2010-12-324210 PMID:21385850 PMCID:PMC3109718
- Ho, M., Feng, M. Q., Fisher, R. J., Rader, C., Pastan, I. A novel high-affinity human monoclonal antibody to mesothelin International Journal of Cancer 2011 128:2020-2030 DOI:10.1002/ijc.25557 PMID:20635390 PMCID:PMC2978266
- Rader, C. DARTs take aim at BiTEs Blood 2011 117:4403-4404 PMID:21527536
- Perez-Galan, P., Mora-Jensen, H., Weniger, M. A., Shaffer, A. L., Rizzatti, E. G., Chapman, C. M., Mo, C. C., Stennett, L. S., Rader, C., Liu, P. C., Raghavachari, N., Stetler-Stevenson, M., et al. Bortezomib resistance in mantle cell lymphoma is associated with plasmacytic differentiation Blood 2011 117:542-552 DOI:10.1182/blood-2010-02-269514 PMID:20956803 PMCID:PMC3031479
- Andris-Widhopf, J., Steinberger, P., Fuller, R., Rader, C., Barbas III, C. F. Generation of human Fab antibody libraries: PCR amplification and assembly of light- and heavy-chain coding sequences Cold Spring Harbor Protocols 2011 2011 DOI:10.1101/pdb.prot065565 PMID:21880817
- Andris-Widhopf, J., Steinberger, P., Fuller, R., Rader, C., Barbas III, C. F. Generation of human scFv antibody libraries: PCR amplification and assembly of light- and heavy-chain coding sequences Cold Spring Harbor Protocols 2011 2011 DOI:10.1101/pdb.prot065573 PMID:21880816
- Hudecek, M., Schmitt, T. M., Baskar, S., Lupo-Stanghellini, M. T., Nishida, T., Yamamoto, T. N., Bleakley, M., Turtle, C. J., Chang, W. C., Greisman, H. A., Wood, B., Maloney, D. G., et al. The B-cell tumor-associated antigen ROR1 can be targeted with T cells modified to express a ROR1-specific chimeric antigen receptor Blood 2010 116:4532-4541 DOI:10.1182/blood-2010-05-283309 PMID:20702778 PMCID:PMC2996114
- Beerli, R. R., Rader, C. Mining human antibody repertoires MAbs 2010 2:365-378 DOI:10.4161/mabs.2.4.12187 PMID:20505349 PMCID:PMC3180084
- Stahl, S. J., Watts, N. R., Rader, C., DiMattia, M. A., Mage, R. G., Palmer, I., Kaufman, J. D., Grimes, J. M., Stuart, D. I., Steven, A. C., Wingfield, P. T. Generation and characterization of a chimeric rabbit/human Fab for co-crystallization of HIV-1 Rev Journal of Molecular Biology 2010 397:697-708 DOI:10.1016/j.jmb.2010.01.061 PMID:20138059 PMCID:PMC2851401
- Lapalombella, R., Andritsos, L., Liu, Q., May, S. E., Browning, R., Pham, L. V., Blum, K. A., Blum, W., Ramanunni, A., Raymond, C. A., Smith, L. L., Lehman, A., et al. Lenalidomide treatment promotes CD154 expression on CLL cells and enhances production of antibodies by normal B cells through a PI3-kinase-dependent pathway Blood 2010 115:2619-2629 DOI:10.1182/blood-2009-09-242438 PMID:19965642 PMCID:PMC2852364
- DiMattia, M. A., Watts, N. R., Stahl, S. J., Rader, C., Wingfield, P. T., Stuart, D. I., Steven, A. C., Grimes, J. M. Implications of the HIV-1 Rev dimer structure at 3.2 Å resolution for multimeric binding to the Rev response element Proceedings of the National Academy of Sciences of the United States of America 2010 107:5810-5814 DOI:10.1073/pnas.0914946107 PMID:20231488 PMCID:PMC2851902
- Salerno, E., Yuan, Y., Scaglione, B. J., Marti, G., Jankovic, A., Mazzella, F., Laurindo, M. F., Despres, D., Baskar, S., Rader, C., Raveche, E. The New Zealand black mouse as a model for the development and progression of chronic lymphocytic leukemia Cytometry Part B-Clinical Cytometry 2010 78B:S98-S109 DOI:10.1002/cyto.b.20544 PMID:20839343 PMCID:PMC2963456
- Hofer, T., Skeffington, L. R., Chapman, C. M., Rader, C. Molecularly defined antibody conjugation through a selenocysteine interface Biochemistry 2009 48:12047-12057 DOI:10.1021/bi901744t PMID:19894757 PMCID:PMC2825887
- Baskar, S., Suschak, J. M., Samija, I., Srinivasan, R., Childs, R. W., Pavletic, S. Z., Bishop, M. R., Rader, C. A human monoclonal antibody drug and target discovery platform for B-cell chronic lymphocytic leukemia based on allogeneic hematopoietic stem cell transplantation and phage display Blood 2009 114:4494-4502 DOI:10.1182/blood-2009-05-222786 PMID:19667400 PMCID:PMC2777128
- Derfuss, T., Parikh, K., Velhin, S., Braun, M., Mathey, E., Krumbholz, M., Kumpfel, T., Moldenhauer, A., Rader, C., Sonderegger, P., Pollmann, W., Tiefenthaller, C., et al. Contactin-2/TAG-1-directed autoimmunity is identified in multiple sclerosis patients and mediates gray matter pathology in animals Proceedings of the National Academy of Sciences of the United States of America 2009 106:8302-8307 DOI:10.1073/pnas.0901496106 PMID:19416878 PMCID:PMC2688870
- Robak, L. A., Venkatesh, K., Lee, H., Raiker, S. J., Duan, Y., Lee-Osbourne, J., Hofer, T., Mage, R. G., Rader, C., Giger, R. J. Molecular basis of the interactions of the Nogo-66 receptor and its homolog NgR2 with myelin-associated glycoprotein: development of NgROMNI-Fc, a novel antagonist of CNS myelin inhibition Journal of Neuroscience 2009 29:5768-5783 DOI:10.1523/jneurosci.4935-08.2009 PMID:19420245 PMCID:PMC2779053
- Kwong, K. Y., Rader, C. E. coli expression and purification of Fab antibody fragments Current Protocols in Protein Science 2009 Chapter 6:6.10.1-6.10.14 DOI:10.1002/0471140864.ps0610s55 PMID:19235139
- Rader, C. Generation and selection of rabbit antibody libraries by phage display Methods in Molecular Biology 2009 525:101-128 DOI:10.1007/978-1-59745-554-1_5 PMID:19252834
- Rader, C. Overview on concepts and applications of Fab antibody fragments Current Protocols in Protein Science 2009 Chapter 6:6.9.1-6.9.14 DOI:10.1002/0471140864.ps0609s55 PMID:19235140
- Kwong, K. Y., Baskar, S., Zhang, H., Mackall, C. L., Rader, C. Generation, affinity maturation, and characterization of a human anti-human NKG2D monoclonal antibody with dual antagonistic and agonistic activity Journal of Molecular Biology 2008 384:1143-1156 DOI:10.1016/j.jmb.2008.09.008 PMID:18809410 PMCID:PMC2659651
- Thomas, J. D., Hofer, T., Rader, C., Burke, T. R. Application of a trifunctional reactive linker for the construction of antibody-drug hybrid conjugates Bioorganic & Medicinal Chemistry Letters 2008 18:5785-5788 DOI:10.1016/j.bmcl.2008.09.078 PMID:18922692 PMCID:PMC3461582
- Hofer, T., Thomas, J. D., Burke, T. R., Rader, C. An engineered selenocysteine defines a unique class of antibody derivatives Proceedings of the National Academy of Sciences of the United States of America 2008 105:12451-12456 DOI:10.1073/pnas.0800800105 PMID:18719095 PMCID:PMC2518826
- Baskar, S., Kwong, K., Hofer, T., Levy, J. M., Kennedy, M. G., Lee, E., Staudt, L. M., Wilson, W. H., Wiestner, A., Rader, C. Unique cell surface expression of receptor tyrosine kinase ROR1 in human B-cell chronic lymphocytic leukemia Clinical Cancer Research 2008 14:396-404 DOI:10.1158/1078-0432.ccr-07-1823 PMID:18223214
- Giger, R. J., Venkatesh, K., Chivatakarn, O., Raiker, S. J., Robak, L., Hofer, T., Lee, H. J., Rader, C. Mechanisms of CNS myelin inhibition: evidence for distinct and neuronal cell type specific receptor systems Restorative Neurology and Neuroscience 2008 26:97-115 PMID:18820405
- Abraham, S., Guo, F., Li, L. S., Rader, C., Liu, C., Barbas, C. F., 3rd, Lerner, R. A., Sinha, S. C. Synthesis of the next-generation therapeutic antibodies that combine cell targeting and antibody-catalyzed prodrug activation Proceedings of the National Academy of Sciences of the United States of America 2007 104:5584-5589 DOI:10.1073/pnas.0700223104 PMID:17372220 PMCID:PMC1838444
- Hofer, T., Tangkeangsirisin, W., Kennedy, M. G., Mage, R. G., Raiker, S. J., Venkatesh, K., Lee, H., Giger, R. J., Rader, C. Chimeric rabbit/human Fab and IgG specific for members of the Nogo-66 receptor family selected for species cross-reactivity with an improved phage display vector Journal of Immunological Methods 2007 318:75-87 DOI:10.1016/j.jim.2006.10.007 PMID:17140598 PMCID:PMC1930160
- Rader, C., Barbas III, C. F. Synthetic antibodies prey on B cells Blood 2006 108:2889-2890 DOI:10.1182/blood-2006-08-041327
- Popkov, M., Rader, C., Gonzalez, B., Sinha, S. C., Barbas, C. F. Small molecule drug activity in melanoma models may be dramatically enhanced with an antibody effector International Journal of Cancer 2006 119:1194-1207 DOI:10.1002/ijc.21924 PMID:16570283
- Jendreyko, N., Rader, C., Barbas III, C. F., Gaedicke, G. Simultaneous, phenotypic knockout of VEGF-R2 and Tie-2 with an intradiabody enhances antiangiogenic effects in vivo Klinische Padiatrie 2006 218:143-151 DOI:10.1055/s-2006-933415 PMID:16688670
- Nathan, S., Rader, C., Barbas, C. F. Neutralization of Burkholderia pseudomallei protease by Fabs generated through phage display Bioscience Biotechnology and Biochemistry 2005 69:2302-2311 DOI:10.1271/bbb.69.2302 PMID:16377887
- Popkov, M., Jendreyko, N., McGavern, D. B., Rader, C., Barbas, C. F. Targeting tumor angiogenesis with adenovirus-delivered anti-Tie-2 intrabody Cancer Research 2005 65:972-981 PMID:15705898
- Venkatesh, K., Chivatakarn, O., Lee, H., Joshi, P. S., Kantor, D. B., Newman, B. A., Mage, R., Rader, C., Giger, R. J. The Nogo-66 receptor homolog NgR2 is a sialic acid-dependent receptor selective for myelin-associated glycoprotein Journal of Neuroscience 2005 25:808-822 DOI:10.1523/jneurosci.4464-04.2005 PMID:15673660
- Jendreyko, N., Popkov, M., Rader, C., Barbas, C. F. Phenotypic knockout of VEGF-R2 and Tie-2 with an intradiabody reduces tumor growth and angiogenesis in vivo Proceedings of the National Academy of Sciences of the United States of America 2005 102:8293-8298 DOI:10.1073/pnas.0503168102 PMID:15928093 PMCID:PMC1149442
- Li, L. S., Rader, C., Matsushita, M., Das, S., Barbas, C. F., Lerner, R. A., Sinha, S. C. Chemical adaptor immunotherapy: design, synthesis, and evaluation of novel integrin-targeting devices Journal of Medicinal Chemistry 2004 47:5630-5640 DOI:10.1021/jm049666k PMID:15509162
- Sinha, S. C., Li, L. S., Watanabe, S., Kaltgrad, E., Tanaka, F., Rader, C., Lerner, R. A., Barbas, C. F. Aldolase antibody activation of prodrugs of potent aldehyde-containing cytotoxics for selective chemotherapy Chemistry-a European Journal 2004 10:5467-5472 DOI:10.1002/chem.200400419 PMID:15378729
- Popkov, M., Rader, C., Barbas, C. F. Isolation of human prostate cancer cell reactive antibodies using phage display technology Journal of Immunological Methods 2004 291:137-151 DOI:10.1016/j.jim.2004.05.004 PMID:15345312
- Popkov, M., Jendreyko, N., Gonzalez-Sapienza, G., Mage, R. G., Rader, C., Barbas, C. F. Human/mouse cross-reactive anti-VEGF receptor 2 recombinant antibodies selected from an immune b9 allotype rabbit antibody library Journal of Immunological Methods 2004 288:149-164 DOI:10.1016/j.jim.2004.03.005 PMID:15183093
- Gopin, A., Rader, C., Shabat, D. New chemical adaptor unit designed to release a drug from a tumor targeting device by enzymatic triggering Bioorganic & Medicinal Chemistry 2004 12:1853-1858 DOI:10.1016/j.bmc.2004.01.41 PMID:15051054
- Sinha, S. C., Li, L. S., Miller, G. P., Dutta, S., Rader, C., Lerner, R. A. Prodrugs of dynemicin analogs for selective chemotherapy mediated by an aldolase catalytic Ab Proceedings of the National Academy of Sciences of the United States of America 2004 101:3095-3099 DOI:10.1073/pnas.0307319101 PMID:14981258 PMCID:PMC365749
- Chung, J. H., Rader, C., Popkov, M., Hur, Y. M., Kim, H. K., Lee, Y. J., Barbas, C. F. Integrin α(IIb)β(3)-specific synthetic human monoclonal antibodies and HCDR3 peptides that potently inhibit platelet aggregation FASEB Journal 2004 18:361-363 DOI:10.1096/fj.03-0586fje PMID:14688205
- Jendreyko, N., Popkov, M., Beerli, R. R., Chung, J. H., McGavern, D. B., Rader, C., Barbas, C. F. Intradiabodies, bispecific, tetravalent antibodies for the simultaneous functional knockout of two cell surface receptors Journal of Biological Chemistry 2003 278:47812-47819 DOI:10.1074/jbc.M307002200 PMID:12947084
- Rader, C., Turner, J. M., Heine, A., Shabat, D., Sinha, S. C., Wilson, I. A., Lerner, R. A., Barbas, C. F. A humanized aldolase antibody for selective chemotherapy and adaptor immunotherapy Journal of Molecular Biology 2003 332:889-899 DOI:10.1016/s0022-2836(03)00992-6 PMID:12972259
- Rader, C., Sinha, S. C., Popkov, M., Lerner, R. A., Barbas, C. F. Chemically programmed monoclonal antibodies for cancer therapy: adaptor immunotherapy based on a covalent antibody catalyst Proceedings of the National Academy of Sciences of the United States of America 2003 100:5396-5400 DOI:10.1073/pnas.0931308100 PMID:12702756 PMCID:PMC154356
- Popkov, M., Mage, R. G., Alexander, C. B., Thundivalappil, S., Barbas, C. F., Rader, C. Rabbit immune repertoires as sources for therapeutic monoclonal antibodies: the impact of kappa allotype-correlated variation in cysteine content on antibody libraries selected by phage display Journal of Molecular Biology 2003 325:325-335 DOI:10.1016/s0022-2836(02)01232-9 PMID:12488098
- Rader, C., Popkov, M., Neves, J. A., Barbas, C. F. Integrin α(v)β(3) targeted therapy for Kaposi's sarcoma with an in vitro evolved antibody FASEB Journal 2002 16:2000-2002 DOI:10.1096/fj.02-0281fje PMID:12397091
- Kunz, B., Lierheimer, R., Rader, C., Spirig, M., Ziegler, U., Sonderegger, P. Axonin-1/TAG-1 mediates cell-cell adhesion by a cis-assisted trans-interaction Journal of Biological Chemistry 2002 277:4551-4557 DOI:10.1074/jbc.M109779200 PMID:11733523
- Plagge, A., Sendtner-Voelderndorff, L., Sirim, P., Freigang, J., Rader, C., Sonderegger, P., Brummendorf, T. The contactin-related protein FAR-2 defines Purkinje cell clusters and labels subpopulations of climbing fibers in the developing cerebellum Molecular and Cellular Neuroscience 2001 18:91-107 DOI:10.1006/mcne.2001.1006 PMID:11461156
- Shabat, D., Lode, H. N., Pertl, U., Reisfeld, R. A., Rader, C., Lerner, R. A., Barbas, C. F. In vivo activity in a catalytic antibody-prodrug system: antibody catalyzed etoposide prodrug activation for selective chemotherapy Proceedings of the National Academy of Sciences of the United States of America 2001 98:7528-7533 DOI:10.1073/pnas.131187998 PMID:11404472 PMCID:PMC34702
- Sonderegger, P., Kunz, S., Rader, C., Suter, D. M., Stoeckli, E. T. Analysis of cell-cell contact mediated by Ig superfamily cell adhesion molecules Current Protocols in Cell Biology 2001 Chapter 9:9.5.1–9.5.52 DOI:10.1002/0471143030.cb0905s11 PMID:18228394
- Rader, C. Antibody libraries in drug and target discovery Drug Discovery Today 2001 6:36-43 DOI:10.1016/s1359-6446(00)01595-6 PMID:11165171
- Steinberger, P., Sutton, J. K., Rader, C., Elia, M., Barbas, C. F. Generation and characterization of a recombinant human CCR5-specific antibody. A phage display approach for rabbit antibody humanization Journal of Biological Chemistry 2000 275:36073-36078 DOI:10.1074/jbc.M002765200 PMID:10969070
- Andris-Widhopf, J., Rader, C., Steinberger, P., Fuller, R., Barbas, C. F. Methods for the generation of chicken monoclonal antibody fragments by phage display Journal of Immunological Methods 2000 242:159-181 DOI:10.1016/s0022-1759(00)00221-0 PMID:10986398
- Rader, C., List, B. Catalytic antibodies as magic bullets Chemistry-a European Journal 2000 6:2091-2095 DOI:10.1002/1521-3765(20000616)6:12<2091::aid-chem2091>3.3.co;2-j PMID:10926212
- Fitzli, D., Stoeckli, E. T., Kunz, S., Siribour, K., Rader, C., Kunz, B., Kozlov, S. V., Buchstaller, A., Lane, R. P., Suter, D. M., Dreyer, W. J., Sonderegger, P. A direct interaction of axonin-1 with NgCAM-related cell adhesion molecule (NrCAM) results in guidance, but not growth of commissural axons Journal of Cell Biology 2000 149:951-968 DOI:10.1083/jcb.149.4.951 PMID:10811834
- Rader, C., Ritter, G., Nathan, S., Elia, M., Gout, I., Jungbluth, A. A., Cohen, L. S., Welt, S., Old, L. J., Barbas, C. F. The rabbit antibody repertoire as a novel source for the generation of therapeutic human antibodies Journal of Biological Chemistry 2000 275:13668-13676 DOI:10.1074/jbc.275.18.13668 PMID:10788485
- Karlstrom, A., Zhong, G. F., Rader, C., Larsen, N. A., Heine, A., Fuller, R., List, B., Tanaka, F., Wilson, I. A., Barbas, C. F., Lerner, R. A. Using antibody catalysis to study the outcome of multiple evolutionary trials of a chemical task Proceedings of the National Academy of Sciences of the United States of America 2000 97:3878-3883 DOI:10.1073/pnas.97.8.3878 PMID:10760259
- Barbas, C. F., Rader, C., Segal, D. J., List, B., Turner, J. M. From catalytic asymmetric synthesis to the transcriptional regulation of genes: in vivo and in vitro evolution of proteins Advances in Protein Chemistry 2000 55:317-366 DOI:10.1016/S0065-3233(01)55008-1 PMID:11050938
- Shabat, D., Rader, C., List, B., Lerner, R. A., Barbas, C. F. Multiple event activation of a generic prodrug trigger by antibody catalysis Proceedings of the National Academy of Sciences of the United States of America 1999 96:6925-6930 DOI:10.1073/pnas.96.12.6925 PMID:10359815
- Kunz, S., Spirig, M., Ginsburg, C., Buchstaller, A., Berger, P., Lanz, R., Rader, C., Vogt, L., Kunz, B., Sonderegger, P. Neurite fasciculation mediated by complexes of axonin-1 and Ng cell adhesion molecule Journal of Cell Biology 1998 143:1673-1690 DOI:10.1083/jcb.143.6.1673 PMID:9852159
- Rader, C., Cheresh, D. A., Barbas, C. F. A phage display approach for rapid antibody humanization: designed combinatorial V gene libraries Proceedings of the National Academy of Sciences of the United States of America 1998 95:8910-8915 DOI:10.1073/pnas.95.15.8910 PMID:9671778
- Sonderegger, P., Kunz, S., Rader, C., Buchstaller, A., Berger, P., Vogt, L., Kozlov, S. V., Ziegler, U., Kunz, B., Fitzli, D., Stoeckli, E. T. Discrete clusters of axonin-1 and NgCAM at neuronal contact sites: facts and speculations on the regulation of axonal fasciculation Progress in Brain Research 1998 117:93-104 PMID:9932403
- Rader, C., Sonderegger, P. Structural features of neural immunoglobulin superfamily adhesion molecules Cell Adhesion and Communication 1998 6:1-22
- Rader, C., Barbas, C. F. Phage display of combinatorial antibody libraries Current Opinion in Biotechnology 1997 8:503-508 DOI:10.1016/s0958-1669(97)80075-4 PMID:9265732
- Buchstaller, A., Kunz, S., Berger, P., Kunz, B., Ziegler, U., Rader, C., Sonderegger, P. Cell adhesion molecules NgCAM and axonin-1 form heterodimers in the neuronal membrane and cooperate in neurite outgrowth promotion Journal of Cell Biology 1996 135:1593-1607 DOI:10.1083/jcb.135.6.1593 PMID:8978825
- Rader, C., Kunz, B., Lierheimer, R., Giger, R. J., Berger, P., Tittmann, P., Gross, H., Sonderegger, P. Implications for the domain arrangement of axonin-1 derived from the mapping of its NgCAM binding site EMBO Journal 1996 15:2056-2068 PMID:8641271 PMCID:PMC450127
- Giger, R. J., Vogt, L., Zuellig, R. A., Rader, C., Henehanbeatty, A., Wolfer, D. P., Sonderegger, P. The gene of chicken axonin-1. Complete structure and analysis of the promoter European Journal of Biochemistry 1995 227:617-628 DOI:10.1111/j.1432-1033.1995.tb20181.x PMID:7867620
- Rader, C., Stoeckli, E. T., Ziegler, U., Osterwalder, T., Kunz, B., Sonderegger, P. Cell-cell adhesion by homophilic interaction of the neuronal recognition molecule axonin-1 European Journal of Biochemistry 1993 215:133-141 DOI:10.1111/j.1432-1033.1993.tb18015.x PMID:8344273
- Hasler, T. H., Rader, C., Stoeckli, E. T., Zuellig, R. A., Sonderegger, P. cDNA cloning, structural features, and eucaryotic expression of human TAG-1/axonin-1 European Journal of Biochemistry 1993 211:329-339 DOI:10.1111/j.1432-1033.1993.tb19902.x PMID:8425542
- Zuellig, R. A., Rader, C., Schroeder, A., Kalousek, M. B., Von Bohlen und Halbach, F., Osterwalder, T., Inan, C., Stoeckli, E. T., Halbach, F., Affolter, H. U., Fritz, A., Hafen, E., et al. The axonally secreted cell adhesion molecule, axonin-1. Primary structure, immunoglobulin-like and fibronectin-type-III-like domains and glycosyl-phosphatidylinositol anchorage European Journal of Biochemistry 1992 204:453-463 DOI:10.1111/j.1432-1033.1992.tb16655.x PMID:1311675
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chapter
- Nanna, A. R., Roush, W. R., Rader, C. Chemical assembly of antibody-drug conjugates Next Generation Antibody Drug Conjugates (ADCs) and Immunotoxins. 1-28. 2017
- Bishop, M. R., Shaffer, B., Rader, C. CD20 Encyclopedia of cancer therapeutic targets. 2012
- Rader, C. Monoclonal antibody therapy for cancer Experimental and applied immunotherapy. 59-83. 2011 DOI:10.1007/978-1-60761-980-2_3
- Rader, C., Bishop, M. R. Monoclonal antibodies in cancer therapy General principles of tumor immunotherapy : basic and clinical applications of tumor immunology. 453-484. 2007 DOI:10.1007/978-1-4020-6087-8_20
- Steinberger, P., Rader, C., Barbas III, C. F. Analysis of selected antibodies Phage display : a laboratory manual. 11.1-11.24. 2001
- Rader, C., Barbas III, C. F. Antibody engineering Phage display : a laboratory manual. 13.1-13.15. 2001
- Andris-Widhopf, J., Steinberger, P., Fuller, R., Rader, C., Barbas III, C. F. Generation of antibody libraries: PCR amplification and assembly of light- and heavy-chain coding sequences Phage display : a laboratory manual. 9.1-9.113. 2001
- Andris-Widhopf, J., Rader, C., Barbas III, C. F. Generation of antibody libraries: immunization, RNA preparation, and cDNA synthesis Phage display : a laboratory manual. 8.1-8.18. 2001
- Rader, C., Steinberger, P., Barbas III, C. F. Selection from antibody libraries Phage display : a laboratory manual. 10.1-10.20. 2001
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patent
- Barbas III, C. F., Shabat, D., Rader, C., List, B., Lerner, R. A., inventors; Scripps Research, assignee. Prodrug activation using catalytic antibodies World patent WO2000071556A1 (2000).