recent publications
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academic article
- Valencia-Gallardo, C., Bou-Nader, C., Aguilar-Salvador, D. T., Carayol, N., Quenech'Du, N., Pecqueur, L., Park, H., Fontecave, M., Izard, T., Van Nhieu, G. T. Shigella IpaA binding to talin stimulates filopodial capture and cell adhesion Cell Reports 2019 26:921-932 DOI:10.1016/j.celrep.2018.12.091 PMID:30673614
- Chinthalapudi, K., Mandati, V., Zheng, J., Sharff, A. J., Bricogne, G., Griffin, P. R., Kissil, J., Izard, T. Lipid binding promotes the open conformation and tumor-suppressive activity of neurofibromin 2 Nature Communications 2018 9 DOI:10.1038/s41467-018-03648-4 PMID:29626191 PMCID:PMC5889391
- Patil, D. N., Rangarajan, E. S., Novick, S. J., Pascal, B. D., Kojetin, D. J., Griffin, P. R., Izard, T., Martemyanov, K. A. Structural organization of a major neuronal G protein regulator, the RGS7-Gβ5-R7BP complex ELife 2018 7 DOI:10.7554/eLife.42150 PMID:30540250 PMCID:PMC6310461
- Chinthalapudi, K., Rangarajan, E. S., Izard, T. The interaction of talin with the cell membrane is essential for integrin activation and focal adhesion formation Proceedings of the National Academy of Sciences of the United States of America 2018 115:10339-10344 DOI:10.1073/pnas.1806275115 PMID:30254158 PMCID:PMC6187153
- Stender, J. D., Nwachukwu, J. C., Kastrati, I., Kim, Y., Strid, T., Yakir, M., Srinivasan, S., Nowak, J., Izard, T., Rangarajan, E. S., Carlson, K. E., Katzenellenbogen, J. A., et al. Structural and molecular mechanisms of cytokine-mediated endocrine resistance in human breast cancer cells Molecular Cell 2017 65:1122-1135 DOI:10.1016/j.molcel.2017.02.008 PMID:28306507
- Nwachukwu, J. C., Srinivasan, S., Bruno, N. E., Nowak, J., Wright, N. J., Minutolo, F., Rangarajan, E. S., Izard, T., Yao, X. Q., Grant, B. J., Kojetin, D. J., Elemento, O., et al. Systems structural biology analysis of ligand effects on ERα predicts cellular response to environmental estrogens and anti-hormone therapies Cell Chemical Biology 2017 24:35-45 DOI:10.1016/j.chembiol.2016.11.014 PMID:28042045
- Cao, W., Kayama, H., Chen, M. L., Delmas, A., Sun, A., Kim, S. Y., Rangarajan, E. S., McKevitt, K., Beck, A. P., Jackson, C. B., Crynen, G., Oikonomopoulos, A., et al. The Xenobiotic Transporter Mdr1 Enforces T Cell Homeostasis in the Presence of Intestinal Bile Acids Immunity 2017 47:1182-1196 DOI:10.1016/j.immuni.2017.11.012 PMID:29262351 PMCID:PMC5741099
- Chinthalapudi, K., Rangarajan, E. S., Brown, D. T., Izard, T. Differential lipid binding of vinculin isoforms promotes quasi-equivalent dimerization Proceedings of the National Academy of Sciences of the United States of America 2016 113:9539-9544 DOI:10.1073/pnas.1600702113 PMID:27503891 PMCID:PMC5003255
- Izard, T., Brown, D. T. Mechanisms and functions of vinculin interactions with phospholipids at cell adhesion sites Journal of Biological Chemistry 2016 291:2548-2555 DOI:10.1074/jbc.R115.686493 PMID:26728462 PMCID:PMC4742724
- Chinthalapudi, K., Patil, D. N., Rangarajan, E. S., Rader, C., Izard, T. Lipid-directed vinculin dimerization Biochemistry 2015 54:2758-2768 DOI:10.1021/acs.biochem.5b00015 PMID:25880222
- Brown, D. T., Izard, T. Vinculin-cell membrane interactions Oncotarget 2015 6:34043-34044 DOI:10.18632/oncotarget.5868 PMID:26431280 PMCID:PMC4741423
- Chinthalapudi, K., Rangarajan, E. S., Patil, D. N., George, E. M., Brown, D. T., Izard, T. Lipid binding promotes oligomerization and focal adhesion activity of vinculin Journal of Cell Biology 2014 207:643-656 DOI:10.1083/jcb.201404128 PMID:25488920 PMCID:PMC4259812
- Poussin, K., Pilati, C., Couchy, G., Calderaro, J., Bioulac-Sage, P., Bacq, Y., Paradis, V., Leteurtre, E., Sturm, N., Ramos, J., Guettier, C., Bardier-Dupas, A., et al. Biochemical and functional analyses of gp130 mutants unveil JAK1 as a novel therapeutic target in human inflammatory hepatocellular adenoma Oncoimmunology 2013 2:e27090 DOI:10.4161/onci.27090 PMID:24501689 PMCID:PMC3913689
- Lee, J. H., Vonrhein, C., Bricogne, G., Izard, T. Crystal structure of the N-terminal domains of the surface cell antigen 4 of Rickettsia Protein Science 2013 22:1425-1431 DOI:10.1002/pro.2322 PMID:23904352 PMCID:PMC3795500
- Rangarajan, E. S., Izard, T. Dimer asymmetry defines alpha-catenin interactions Nature Structural & Molecular Biology 2013 20:188-193 DOI:10.1038/nsmb.2479 PMID:23292143 PMCID:PMC3805043
- Yogesha, S. D., Rangarajan, E. S., Vonrhein, C., Bricogne, G., Izard, T. Crystal structure of vinculin in complex with vinculin binding site 50 (VBS50), the integrin binding site 2 (IBS2) of talin Protein Science 2012 21:583-588 DOI:10.1002/pro.2041 PMID:22334306 PMCID:PMC3375758
- Rangarajan, E. S., Izard, T. The cytoskeletal protein alpha-catenin unfurls upon binding to vinculin Journal of Biological Chemistry 2012 287:18492-18499 DOI:10.1074/jbc.M112.351023 PMID:22493458 PMCID:PMC3365723
- Lee, J. H., Rangarajan, E. S., Vonrhein, C., Bricogne, G., Izard, T. The metavinculin tail domain directs constitutive interactions with raver1 and vinculin RNA Journal of Molecular Biology 2012 422:697-704 DOI:10.1016/j.jmb.2012.06.015 PMID:22709580 PMCID:PMC3835166
- Rangarajan, E. S., Lee, J. H., Izard, T. Apo raver1 structure reveals distinct RRM domain orientations Protein Science 2011 20:1464-1470 DOI:10.1002/pro.664 PMID:21633983 PMCID:PMC3189531
- Yogesha, S. D., Sharff, A., Bricogne, G., Izard, T. Intermolecular versus intramolecular interactions of the vinculin binding site 33 of talin Protein Science 2011 20:1471-1476 DOI:10.1002/pro.671 PMID:21648001 PMCID:PMC3189532
- Park, H., Valencia-Gallardo, C., Sharff, A., Guy, T. V. N., Izard, T. Novel vinculin binding site of the IpaA invasin of Shigella Journal of Biological Chemistry 2011 286:23214-23221 DOI:10.1074/jbc.M110.184283 PMID:21525010 PMCID:PMC3123088
- Pilati, C., Amessou, M., Bihl, M. P., Balabaud, C., Jeanne, T. V. N., Paradis, V., Nault, J. C., Izard, T., Bioulac-Sage, P., Couchy, G., Poussin, K., Zucman-Rossi, J. Somatic mutations activating STAT3 in human inflammatory hepatocellular adenomas Journal of Experimental Medicine 2011 208:1359-1366 DOI:10.1084/jem.20110283 PMID:21690253 PMCID:PMC3135371
- Park, H., Lee, J. H., Gouin, E., Cossart, P., Izard, T. The rickettsia surface cell antigen 4 applies mimicry to bind to and activate vinculin Journal of Biological Chemistry 2011 286:35096-35103 DOI:10.1074/jbc.M111.263855 PMID:21841197 PMCID:PMC3186400
- Yogesha, S. D., Sharff, A. J., Giovannini, M., Bricogne, G., Izard, T. Unfurling of the band 4.1, ezrin, radixin, moesin (FERM) domain of the merlin tumor suppressor Protein Science 2011 20:2113-2120 DOI:10.1002/pro.751 PMID:22012890 PMCID:PMC3302654
- Rangarajan, E. S., Lee, J. H., Yogesha, S. D., Izard, T. A helix replacement mechanism directs metavinculin functions PLoS One 2010 5:e10679 DOI:10.1371/journal.pone.0010679 PMID:20502710 PMCID:PMC2873289
- Park, H., Rangarajan, E. S., Sygusch, J., Izard, T. Dramatic improvement of crystal quality for low-temperature-grown rabbit muscle aldolase Acta Crystallographica Section F-Structural Biology and Crystallization Communications 2010 66:595-600 DOI:10.1107/s1744309110011875 PMID:20445268 PMCID:PMC2864701
- Rangarajan, E. S., Izard, T. Improving the diffraction of full-length human selenomethionyl metavinculin crystals by streak-seeding Acta Crystallographica Section F-Structural Biology and Crystallization Communications 2010 66:1617-1620 DOI:10.1107/s1744309110041059 PMID:21139209 PMCID:PMC2998368
- Rangarajan, E. S., Park, H., Fortin, E., Sygusch, J., Izard, T. Mechanism of aldolase control of sorting nexin 9 function in endocytosis Journal of Biological Chemistry 2010 285:11983-11990 DOI:10.1074/jbc.M109.092049 PMID:20129922 PMCID:PMC2852936
- George, E. M., Izard, T., Anderson, S. D., Brown, D. T. Nucleosome interaction surface of linker histone H1c is distinct from that of H1(0) Journal of Biological Chemistry 2010 285:20891-20896 DOI:10.1074/jbc.M110.108639 PMID:20444700 PMCID:PMC2898364
- Rebouissou, S., Amessou, M., Couchy, G., Poussin, K., Imbeaud, S., Pilati, C., Izard, T., Balabaud, C., Bioulac-Sage, P., Zucman-Rossi, J. Frequent in-frame somatic deletions activate gp130 in inflammatory hepatocellular tumours Nature 2009 457:200-204 DOI:10.1038/nature07475 PMID:19020503 PMCID:PMC2695248
- Lee, J. H., Rangarajan, E. S., Yogesha, S. D., Izard, T. Raver1 interactions with vinculin and RNA suggest a feed-forward pathway in directing mRNA to focal adhesions Structure 2009 17:833-842 DOI:10.1016/j.str.2009.04.010 PMID:19523901 PMCID:PMC2811071
- St-Jean, M., Izard, T., Sygusch, J. A hydrophobic pocket in the active site of glycolytic aldolase mediates interactions with Wiskott-Aldrich syndrome protein Journal of Biological Chemistry 2007 282:14309-14315 DOI:10.1074/jbc.M611505200 PMID:17329259
- Ramarao, N., Le Clainche, C., Izard, T., Bourdet-Sicard, R., Ageron, E., Sansonetti, P. J., Carlier, M. F., Van Nhieu, G. T. Capping of actin filaments by vinculin activated by the Shigella IpaA carboxyl-terminal domain FEBS Letters 2007 581:853-857 DOI:10.1016/j.febslet.2007.01.057 PMID:17289036
- Van Nhieu, G. T., Izard, T. Vinculin binding in its closed conformation by a helix addition mechanism EMBO Journal 2007 26:4588-4596 DOI:10.1038/sj.emboj.7601863 PMID:17932491 PMCID:PMC2063484
- Brown, D. T., Izard, T., Misteli, T. Mapping the interaction surface of linker histone H1(0) with the nucleosome of native chromatin in vivo Nature Structural & Molecular Biology 2006 13:250-255 DOI:10.1038/nsmb1050 PMID:16462749 PMCID:PMC1868459
- Izard, T., Van Nhieu, G. T., Bois, P. R. J. Shigella applies molecular mimicry to subvert vinculin and invade host cells Journal of Cell Biology 2006 175:465-475 DOI:10.1083/jcb.200605091 PMID:17088427 PMCID:PMC2064523
- Bois, P. R. J., O'Hara, B. P., Nietlispach, D., Kirkpatrick, J., Izard, T. The vinculin binding sites of talin and alpha-actinin are sufficient to activate vinculin Journal of Biological Chemistry 2006 281:7228-7236 DOI:10.1074/jbc.M510397200 PMID:16407299
- Bois, P. R. J., Borgon, R. A., Vonrhein, C., Izard, T. Structural dynamics of alpha-actinin-vinculin interactions Molecular and Cellular Biology 2005 25:6112-6122 DOI:10.1128/mcb.25.14.6112-6122.2005 PMID:15988023
- Borgon, R. A., Vonrhein, C., Bricogne, G., Bois, P. R. J., Izard, T. Crystal structure of human vinculin Structure 2004 12:1189-1197 DOI:10.1016/j.str.2004.05.009 PMID:15242595
- Izard, T., Sygusch, J. Induced fit movements and metal cofactor selectivity of class ll aldolases - structure of Thermus aquaticus fructose-1,6-bisphosphate aldolase Journal of Biological Chemistry 2004 279:11825-11833 DOI:10.1074/jbc.M311375200 PMID:14699122
- Brown, K. L., Morris, V. K., Izard, T. Rhombohedral crystals of Mycobacterium tuberculosis phosphopantetheine adenylyltransferase Acta Crystallographica Section D-Biological Crystallography 2004 60:195-196 DOI:10.1107/s0907444903025988 PMID:14684928
- Rush, C. L., Izard, T. Rhombohedral crystals of the human vinculin head domain in complex with a vinculin-binding site of talin Acta Crystallographica Section D-Biological Crystallography 2004 60:945-947 DOI:10.1107/s0907444904006547 PMID:15103147
- Izard, T., Vonrhein, C. Structural basis for amplifying vinculin activation by talin Journal of Biological Chemistry 2004 279:27667-27678 DOI:10.1074/jbc.M403076200 PMID:15070891
- Morris, V. K., Izard, T. Substrate-induced asymmetry and channel closure revealed by the apoenzyme structure of Mycobacterium tuberculosis phosphopantetheine adenylyltransferase Protein Science 2004 13:2547-2552 DOI:10.1110/ps.04816904 PMID:15322293 PMCID:PMC2280004
- Izard, T., Evans, G., Borgon, R. A., Rush, C. L., Bricogne, G., Bois, P. R. J. Vinculin activation by talin through helical bundle conversion Nature 2004 427:171-175 DOI:10.1038/nature02281 PMID:14702644
- Izard, T. A novel adenylate binding site confers phosphopantetheine adenylyltransferase interactions with coenzyme A Journal of Bacteriology 2003 185:4074-4080 DOI:10.1128/jb.185.14.4074-4080.2003 PMID:12837781
- Hurlbert, J. C., Izard, T. Crystallization of HLA-DR4 fused to an immunodominant collagen ll peptide implicated in rheumatoid arthritis Acta Crystallographica Section D-Biological Crystallography 2002 58:1749-1751 DOI:10.1107/s0907444902014142 PMID:12351899
- Izard, T. The crystal structures of phosphopantetheine adenylyltransferase with bound substrates reveal the enzyme's catalytic mechanism Journal of Molecular Biology 2002 315:487-495 DOI:10.1006/jmbi.2001.5272 PMID:11812124
- Izard, T. Structural basis for chloramphenicol tolerance in Streptomyces venezuelae by chloramphenicol phosphotransferase activity Protein Science 2001 10:1508-1513 DOI:10.1110/ps.10.8.1508 PMID:11468347
- Izard, T., Blackwell, N. C. Crystal structures of the metal-dependent 2-dehydro-3-deoxy-galactarate aldolase suggest a novel reaction mechanism EMBO Journal 2000 19:3849-3856 DOI:10.1093/emboj/19.15.3849 PMID:10921867 PMCID:PMC306599
- Izard, T., Ellis, J. The crystal structures of chloramphenicol phosphotransferase reveal a novel inactivation mechanism EMBO Journal 2000 19:2690-2700 DOI:10.1093/emboj/19.11.2690 PMID:10835366 PMCID:PMC212772
- Ellis, J., Campopiano, D. J., Izard, T. Cubic crystals of chloramphenicol phosphotransferase from Streptomyces venezuelae in complex with chloramphenicol Acta Crystallographica Section D-Biological Crystallography 1999 55:1086-1088 DOI:10.1107/s0907444999003029 PMID:10216290
- Izard, T., Geerlof, A., Lewendon, A., Barker, J. J. Cubic crystals of phosphopantetheine adenylyltransferase from Escherichia coli Acta Crystallographica Section D-Biological Crystallography 1999 55:1226-1228 DOI:10.1107/s0907444999004394 PMID:10329792
- Izard, T., Aevarsson, A., Allen, M. D., Westphal, A. H., Perham, R. N., de Kok, A., Hol, W. G. J. Principles of quasi-equivalence and Euclidean geometry govern the assembly of cubic and dodecahedral cores of pyruvate dehydrogenase complexes Proceedings of the National Academy of Sciences of the United States of America 1999 96:1240-1245 DOI:10.1073/pnas.96.4.1240 PMID:9990008
- Blackwell, N. C., Cullis, P. M., Cooper, R. A., Izard, T. Rhombohedral crystals of 2-dehydro-3-deoxygalactarate aldolase from Escherichia coli Acta Crystallographica Section D-Biological Crystallography 1999 55:1368-1369 DOI:10.1107/s0907444999006502 PMID:10393309
- Izard, T., Geerlof, A. The crystal structure of a novel bacterial adenylyltransferase reveals half of sites reactivity EMBO Journal 1999 18:2021-2030 DOI:10.1093/emboj/18.8.2021 PMID:10205156 PMCID:PMC1171286
- Izard, T., Sarfaty, S., Westphal, A., DeKok, A., Hol, W. G. J. Improvement of diffraction quality upon rehydration of dehydrated icosahedral Enterococcus faecalis pyruvate dehydrogenase core crystals Protein Science 1997 6:913-915 DOI:10.1002/pro.5560060419 PMID:9098902 PMCID:PMC2144755
- Lawrence, M. C., Izard, T., Beuchat, M., Blagrove, R. J., Colman, P. M. Structure of phaseolin at 2.2 Å resolution - implications for a common vicilin/legumin structure and the genetic-engineering of seed storage proteins Journal of Molecular Biology 1994 238:748-776 DOI:10.1006/jmbi.1994.1333 PMID:8182747
- Izard, T., Lawrence, M. C., Malby, R. L., Lilley, G. G., Colman, P. M. The three-dimensional structure of N-acetylneuraminate lyase from Escherichia coli Structure 1994 2:361-369 DOI:10.1016/s0969-2126(00)00038-1 PMID:8081752
- Izard, T., Fol, B., Pauptit, R. A., Jansonius, J. N. Trigonal crystals of porcine mitochondrial aspartate-aminotransferase Journal of Molecular Biology 1990 215:341-344 DOI:10.1016/s0022-2836(05)80355-9 PMID:2231709
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