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Gascoigne, Nicholas
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Gascoigne, Nicholas

Faculty Member Adjunct
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Positions

  • 2017 - Professor Adjunct, Immunology and Microbiology , Scripps Research
  • 2013 - Head of Department and Professor, Department of Microbiology & Immunology, National University of Singapore
  • 2013 - 2017 Professor Adjunct, Immunology and Microbial Science (IMS) , Scripps Research
  • 1987 - 2013 Professor, Immunology and Microbial Science (IMS) , Scripps Research
  • 1987 - 2013 Faculty Member, Skaggs Graduate School of Chemical and Biological Sciences , Scripps Research
  • 1983 - 1987 Postdoctoral Fellow, School of Medicine, Stanford University
Dr. Nicholas Gascoigne examines molecular and genetic interactions in T cell development and activation, including the spatiotemporal analysis of signaling cascades using fluorescence resonance energy transfer (FRET) and other techniques to image molecular interactions in live cells.

Contact

  • gascoign@scripps.edu

Websites

  • Nicholas Gascoigne, Ph.D.
  • Laboratory Website
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Publications

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    • Prasad, M., Wojciech, L., Brzostek, J., Hu, J. F., Chua, Y. L., Tung, D. W. H., Yap, J., Rybakin, V., Gascoigne, N. R. J. Expansion of an Unusual Virtual Memory CD8(+) Subpopulation Bearing V alpha 3.2 TCR in Themis-Deficient Mice Frontiers in Immunology  2021 12  DOI:10.3389/fimmu.2021.644483  PMID:33897691  PMCID:PMC8058184
    • Paster, W., Bruger, A. M., Katsch, K., Gregoire, C., Roncagalli, R., Fu, G., Gascoigne, N. R. J., Nika, K., Cohnen, A., Feller, S. M., Simister, P. C., Molder, K. C., et al. A THEMIS:SHP1 complex promotes T-cell survival EMBO Journal  2015 34:393-409  DOI:10.15252/embj.201387725  PMID:25535246  PMCID:PMC4339124
    • Rybakin, V., Westernberg, L., Fu, G., Kim, H. O., Ampudia, J., Sauer, K., Gascoigne, N. R. J. Allelic exclusion of TCR α-chains upon severe restriction of Vα repertoire PLoS One  2014 9  DOI:10.1371/journal.pone.0114320  PMID:25500569  PMCID:PMC4264757
    • Fu, G., Rybakin, V., Brzostek, J., Paster, W., Acuto, O., Gascoigne, N. R. J. Fine-tuning T cell receptor signaling to control T cell development Trends in Immunology  2014 35:311-318  DOI:10.1016/j.it.2014.05.003  PMID:24951034  PMCID:PMC4119814
    • Kong, K. F., Fu, G., Zhang, Y., Yokosuka, T., Casas, J., Canonigo-Balancio, A. J., Becart, S., Kim, G., Yates III, J. R., Kronenberg, M., Saito, T., Gascoigne, N. R. J., et al. Protein kinase C-eta controls CTLA-4-mediated regulatory T cell function Nature Immunology  2014 15:465-472  DOI:10.1038/ni.2866  PMID:24705298  PMCID:PMC4040250
    • Nabekura, T., Kanaya, M., Shibuya, A., Fu, G., Gascoigne, N. R. J., Lanier, L. L. Costimulatory molecule DNAM-1 is essential for optimal differentiation of memory natural killer cells during mouse cytomegalovirus infection Immunity  2014 40:225-234  DOI:10.1016/j.immuni.2013.12.011  PMID:24440149  PMCID:PMC3943894
    • Casas, J., Brzostek, J., Zarnitsyna, V. I., Hong, J. S., Wei, Q., Hoerter, J. A. H., Fu, G., Ampudia, J., Zamoyska, R., Zhu, C., Gascoigne, N. R. J. Ligand-engaged TCR is triggered by Lck not associated with CD8 coreceptor Nature Communications  2014 5:5624  DOI:10.1038/ncomms6624  PMID:25427562  PMCID:PMC4248239
    • Fu, G., Casas, J., Rigaud, S., Rybakin, V., Lambolez, F., Brzostek, J., Hoerter, J. A. H., Paster, W., Acuto, O., Cheroutre, H., Sauer, K., Gascoigne, N. R. J. Themis sets the signal threshold for positive and negative selection in T-cell development Nature  2013 504:441-445  DOI:10.1038/nature12718  PMID:24226767  PMCID:PMC3977001
    • Mukherjee, S., Rigaud, S., Seok, S. C., Fu, G., Prochenka, A., Dworkin, M., Gascoigne, N. R. J., Vieland, V. J., Sauer, K., Das, J. In silico modeling of Itk activation kinetics in thymocytes suggests competing positive and negative IP4 mediated feedbacks increase robustness PLoS One  2013 8:e73937  DOI:10.1371/journal.pone.0073937  PMID:24066087  PMCID:PMC3774804
    • Hoerter, J. A. H., Brzostek, J., Artyomov, M. N., Abel, S. M., Casas, J., Rybakin, V., Ampudia, J., Lotz, C., Connolly, J. M., Chakraborty, A. K., Gould, K. G., Gascoigne, N. R. J. Coreceptor affinity for MHC defines peptide specificity requirements for TCR interaction with coagonist peptide-MHC Journal of Experimental Medicine  2013 210:1807-1821  DOI:10.1084/jem.20122528  PMID:23940257  PMCID:PMC3754861
    • Paster, W., Brockmeyer, C., Fu, G., Simister, P. C., de Wet, B., Martinez-Riano, A., Hoerter, J. A. H., Feller, S. M., Wulfing, C., Gascoigne, N. R. J., Acuto, O. GRB2-mediated recruitment of THEMIS to LAT is essential for thymocyte development Journal of Immunology  2013 190:3749-3756  DOI:10.4049/jimmunol.1203389  PMID:23460737  PMCID:PMC3607403
    • Rybakin, V., Gascoigne, N. R. J. Negative selection assay based on stimulation of t cell receptor transgenic thymocytes with peptide-mhc tetramers PLoS One  2012 7:e43191  DOI:10.1371/journal.pone.0043191  PMID:22900100  PMCID:PMC3416795
    • Fu, G., Hu, J. F., Niederberger-Magnenat, N., Rybakin, V., Casas, J., Yachi, P. P., Feldstein, S., Ma, B., Hoerter, J. A. H., Ampudia, J., Rigaud, S., Lambolez, F., et al. Protein kinase C η is required for T cell activation and homeostatic proliferation Science Signaling  2011 4:ra84  DOI:10.1126/scisignal.2002058  PMID:22155788  PMCID:PMC3242502
    • Rybakin, V., Clamme, J. P., Ampudia, J., Yachi, P. P., Gascoigne, N. R. J. CD8αα and ‐αβ isotypes are equally recruited to the immunological synapse through their ability to bind to MHC class I EMBO Reports  2011 12:1251-1256  DOI:10.1038/embor.2011.209  PMID:22081144  PMCID:PMC3245696
    • Gascoigne, N. R. J., Palmer, E. Signaling in thymic selection Current Opinion in Immunology  2011 23:207-212  DOI:10.1016/j.coi.2010.12.017  PMID:21242076  PMCID:PMC3073816
    • Brockmeyer, C., Paster, W., Pepper, D., Tan, C. P., Trudgian, D. C., McGowan, S., Fu, G., Gascoigne, N. R. J., Acuto, O., Salek, M. T cell receptor (TCR)-induced tyrosine phosphorylation dynamics identifies THEMIS as a new TCR signalosome component Journal of Biological Chemistry  2011 286:7535-7547  DOI:10.1074/jbc.M110.201236  PMID:21189249  PMCID:PMC3045008
    • Gascoigne, N. R. J., Zal, T., Yachi, P. P., Hoerter, J. A. H. Co-receptors and recognition of self at the immunological synapse Current Topics in Microbiology and Immunology  2010 340:171-189  DOI:10.1007/978-3-642-03858-7_9  PMID:19960314
    • Fu, G., Gascoigne, N. R. J. Multiplexed labeling of samples with cell tracking dyes facilitates rapid and accurate internally controlled calcium flux measurement by flow cytometry Journal of Immunological Methods  2009 350:194-199  DOI:10.1016/j.jim.2009.07.009  PMID:19647745  PMCID:PMC2782925
    • Fu, G., Vallee, S., Rybakin, V., McGuire, M. V., Ampudia, J., Brockmeyer, C., Salek, M., Fallen, P. R., Hoerter, J. A. H., Munshi, A., Huang, Y. H., Hu, J. F., et al. Themis controls thymocyte selection through regulation of t cell antigen receptor-mediated signaling Nature Immunology  2009 10:848-856  DOI:10.1038/ni.1766  PMID:19597499  PMCID:PMC2757056
    • Singleton, K. L., Roybal, K. T., Sun, Y., Fu, G., Gascoigne, N. R. J., van Oers, N. S. C., Wulfing, C. Spatiotemporal patterning during t cell activation is highly diverse Science Signaling  2009 2:ra15  DOI:10.1126/scisignal.2000199  PMID:19351954  PMCID:PMC2694444
    • Gascoigne, N. R. J., Ampudia, J., Clamme, J. P., Fu, G., Lotz, C., Mallaun, M., Niederberger, N., Palmer, E., Rybakin, V., Yachi, P. P., Zal, T. Visualizing intermolecular interactions in T cells Current Topics in Microbiology and Immunology  2009 334:31-46  DOI:10.1007/978-3-540-93864-4_2  PMID:19521680  PMCID:PMC3079427
    • Gascoigne, N. R. J. Do t cells need endogenous peptides for activation? Nature Reviews Immunology  2008 8:895-900  DOI:10.1038/nri2431  PMID:18846098
    • Mallaun, M., Naeher, D., Daniels, M. A., Yachi, P. P., Hausmann, B., Luescher, I. F., Gascoigne, N. R. J., Palmer, E. The T cell receptor's α-chain connecting peptide motif promotes close approximation of the CD8 coreceptor allowing efficient signal initiation Journal of Immunology  2008 180:8211-8221  PMID:18523287  PMCID:PMC2764738
    • Haraldsson, M. K., Louis-Dit-Sully, C. A., Lawson, B. R., Sternik, G., Santiago-Raber, M. L., Gascoigne, N. R. J., Theofilopoulos, A. N., Kono, D. H. The lupus-related Lmb3 locus contains a disease-suppressing coronin-1A gene mutation Immunity  2008 28:40-51  DOI:10.1016/j.immuni.2007.11.023  PMID:18199416  PMCID:PMC2274909
    • Yachi, P. P., Lotz, C., Ampudia, J., Gascoigne, N. R. J. T cell activation enhancement by endogenous pMHC acts for both weak and strong agonists but varies with differentiation state Journal of Experimental Medicine  2007 204:2747-2757  DOI:10.1084/jem.20062610  PMID:17954567  PMCID:PMC2118480
    • Daniels, M. A., Teixeiro, E., Gill, J., Hausmann, B., Roubaty, D., Holmberg, K., Werlen, G., Hollander, G. A., Gascoigne, N. R. J., Palmer, E. Thymic selection threshold defined by compartmentalization of ras/mapk signalling Nature  2006 444:724-729  DOI:10.1038/nature05269  PMID:17086201
    • Zal, T., Zal, M. A., Lotz, C., Goergen, C. J., Gascoigne, N. R. J. Spectral shift of fluorescent dye fm4-64 reveals distinct microenvironment of nuclear envelope in living cells Traffic  2006 7:1607-1613  DOI:10.1111/j.1600-0854.2006.00498.x  PMID:17052249
    • Zambricki, E., Zal, T., Yachi, P., Shigeoka, A., Sprent, J., Gascoigne, N., McKay, D. In vivo anergized T cells form altered immunological synapses in vitro American Journal of Transplantation  2006 6:2572-2579  DOI:10.1111/j.1600-6143.2006.01517.x  PMID:16952297
    • Yachi, P. P., Ampudia, J., Zal, T., Gascoigne, N. R. J. Altered peptide ligands induce delayed CD8-T cell receptor interaction--a role for CD8 in distinguishing antigen quality Immunity  2006 25:203-211  DOI:10.1016/j.immuni.2006.05.015  PMID:16872849
    • Bosco, N., Hung, H. C., Pasqual, N., Jouvin-Marche, E., Marche, P. N., Gascoigne, N. R. J., Ceredig, R. Role of the t cell receptor alpha chain in the development and phenotype of naturally arising cd4(+)cd25(+) t cells Molecular Immunology  2006 43:246-254  DOI:10.1016/j.molimm.2005.02.002  PMID:16199261
    • Yachi, P. P., Ampudia, J., Gascoigne, N. R. J., Zal, T. Nonstimulatory peptides contribute to antigen-induced CD8-T cell receptor interaction at the immunological synapse Nature Immunology  2005 6:785-792  DOI:10.1038/ni1220  PMID:15980863  PMCID:PMC1352171
    • Lin, M. Y., Zal, T., Ch'en, I. L., Gascoigne, N. R. J., Hedrick, S. M. A pivotal role for the multifunctional calcium/calmodulin-dependent protein kinase ii in t cells: From activation to unresponsiveness Journal of Immunology  2005 174:5583-5592  PMID:15843557
    • Niederberger, N., Buehler, L. K., Ampudia, J., Gascoigne, N. R. J. Thymocyte stimulation by anti-tcr-beta, but not by anti-tcr-alpha, leads to induction of developmental transcription program Journal of Leukocyte Biology  2005 77:830-841  DOI:10.1189/jlb.1004608  PMID:15661827
    • Gronski, M. A., Boulter, J. M., Moskophidis, D., Nguyen, L. T., Holmberg, K., Elford, A. R., Deenick, E. K., Kim, H. O., Penninger, J. M., Odermatt, B., Gallimore, A., Gascoigne, N. R. J., et al. Tcr affinity and negative regulation limit autoimmunity Nature Medicine  2004 10:1234-1239  DOI:10.1038/nm1114  PMID:15467726
    • Santori, F. R., Holmberg, K., Ostrov, D., Gascoigne, N. R. J., Vukmanovic, S. Distinct footprints of tcr engagement with highly homologous ligands Journal of Immunology  2004 172:7466-7475  PMID:15187125
    • Gascoigne, N. R. J., Zal, T. Molecular interactions at the t cell-antigen-presenting cell interface Current Opinion in Immunology  2004 16:114-119  DOI:10.1016/j.coi.2003.11.008  PMID:14734119
    • Zal, T., Gascoigne, N. R. J. Photobleaching-corrected fret efficiency imaging of live cells Biophysical Journal  2004 86:3923-3939  DOI:10.1529/biophysj.103.022087  PMID:15189889  PMCID:PMC1304294
    • Zal, T., Gascoigne, N. R. Using live fret imaging to reveal early protein-protein interactions during t cell activation Current Opinion in Immunology  2004 16:418-427  DOI:10.1016/j.coi.2004.05.019  PMID:15245734
    • Holmberg, K., Mariathasan, S., Ohteki, T., Ohashi, P. S., Gascoigne, N. R. J. Tcr binding kinetics measured with mhc class i tetramers reveal a positive selecting peptide with relatively high affinity for tcr Journal of Immunology  2003 171:2427-2434  PMID:12928390
    • Niederberger, N., Holmberg, K., Alam, S. M., Sakati, W., Naramura, M., Gu, H., Gascoigne, N. R. J. Allelic exclusion of the tcr alpha-chain is an active process requiring tcr-mediated signaling and c-cbl Journal of Immunology  2003 170:4557-4563  PMID:12707333
    • Sim, B. C., Holmberg, K., Sidobre, S., Naidenko, O., Niederberger, N., Marine, S. D., Kronenberg, M., Gascoigne, N. R. J. Surprisingly minor influence of trav11 (v alpha 14) polymorphism on nk t-receptor mcd1/alpha-galactosylceramide binding kinetics Immunogenetics  2003 54:874-883  DOI:10.1007/s00251-002-0532-y  PMID:12671739
    • Sidobre, S., Naidenko, O. V., Sim, B. C., Gascoigne, N. R. J., Garcia, K. C., Kronenberg, M. The v alpha 14 nkt cell tcr exhibits high-affinity binding to a glycolipid/cd1d complex Journal of Immunology  2002 169:1340-1348  PMID:12133957
    • Huang, J. Y., Lo, P. F., Zal, T., Gascoigne, N. R. J., Smith, B. A., Levin, S. D., Grey, H. M. Cd28 plays a critical role in the segregation of pkc theta within the immunologic synapse Proceedings of the National Academy of Sciences of the United States of America  2002 99:9369-9373  DOI:10.1073/pnas.142298399  PMID:12077322  PMCID:PMC123147
    • Zal, T., Zal, M. A., Gascoigne, N. R. J. Inhibition of t cell receptor-coreceptor interactions by antagonist ligands visualized by live fret imaging of the t-hybridoma immunological synapse Immunity  2002 16:521-534  DOI:10.1016/s1074-7613(02)00301-1  PMID:11970876
    • McGuire, M. V., Suthipinijtham, P., Gascoigne, N. R. J. The mouse supt16h/fact140 gene, encoding part of the fact chromatin transcription complex, maps close to tcra and is highly expressed in thymus Mammalian Genome  2001 12:664-667  DOI:10.1007/s003350020036  PMID:11471063
    • Rosette, C., Werlen, G., Daniels, M. A., Holman, P. O., Alam, S. M., Travers, P. J., Gascoigne, N. R. J., Palmer, E., Jameson, S. C. The impact of duration versus extent of tcr occupancy on t cell activation: A revision of the kinetic proofreading model Immunity  2001 15:59-70  DOI:10.1016/s1074-7613(01)00173-x  PMID:11485738
    • Redpath, S., Ghazal, P., Gascoigne, N. R. J. Hijacking and exploitation of il-10 by intracellular pathogens Trends in Microbiology  2001 9:86-92  DOI:10.1016/s0966-842x(00)01919-3  PMID:11173248
    • Redpath, S., Angulo, A., Gascoigne, N. R. J., Ghazal, P. Immune checkpoints in viral latency Annual Review of Microbiology  2001 55:531-560  DOI:10.1146/annurev.micro.55.1.531  PMID:11544366
    • Gascoigne, N. R. J., Alam, S. M., Lin, C. M., McGuire, M. V., Marine, S., Niederberger, N., Redpath, S., Sim, B. C., Travers, P. J., Yachi, P., Zal, M. A., Zal, T. T cell receptor binding kinetics and special role of Vα in T cell development and activation Immunologic Research  2000 21:225-231  DOI:10.1385/ir:21:2-3:225  PMID:10852121
    • Gascoigne, N. R. J., Alam, S. M. Allelic exclusion of the t cell receptor alpha-chain: Developmental regulation of a post-translational event Seminars in Immunology  1999 11:337-347  DOI:10.1006/smim.1999.0190  PMID:10497088
    • Redpath, S., Alam, S. M., Lin, C. M., O'Rourke, A. M., Gascoigne, N. R. J. Cutting edge: Trimolecular interaction of tcr with mhc class ii and bacterial superantigen shows a similar affinity to mhc : Peptide ligands Journal of Immunology  1999 163:6-10  PMID:10384091
    • Redpath, S., Angulo, A., Gascoigne, N. R. J., Ghazal, P. Murine cytomegalovirus infection down-regulates mhc class ii expression on macrophages by induction of il-10 Journal of Immunology  1999 162:6701-6707  PMID:10352288
    • Sim, B. C., Gascoigne, N. R. J. Reciprocal expression in cd4 or cd8 subsets of different members of the v alpha 11 gene family correlates with sequence polymorphism Journal of Immunology  1999 162:3153-3159  PMID:10092765
    • Alam, S. M., Davies, G. M., Lin, C. M., Zal, T., Nasholds, W., Jameson, S. C., Hogquist, K. A., Gascoigne, N. R. J., Travers, P. J. Qualitative and quantitative differences in t cell receptor binding of agonist and antagonist ligands Immunity  1999 10:227-237  DOI:10.1016/s1074-7613(00)80023-0  PMID:10072075
    • Sim, B. C., Aftahi, N., Reilly, C., Bogen, B., Schwartz, R. H., Gascoigne, N. R. J., Lo, D. Thymic skewing of the cd4/cd8 ratio maps with the t-cell receptor alpha-chain locus Current Biology  1998 8:701-704  DOI:10.1016/s0960-9822(98)70276-3  PMID:9637921
    • Sim, B. C., Lo, D., Gascoigne, N. R. J. Preferential expression of tcr v alpha regions in cd4/cd8 subsets: Class discrimination or co-receptor recognition? Immunology Today  1998 19:276-282  DOI:10.1016/s0167-5699(98)01257-2  PMID:9639993
    • Alam, S. M., Gascoigne, N. R. J. Posttranslational regulation of tcr v alpha allelic exclusion during t cell differentiation Journal of Immunology  1998 160:3883-3890  PMID:9558094
    • Sim, B. C., Wung, J. L., Gascoigne, N. R. J. Polymorphism within a tcrav family influences the repertoire through class i/ii restriction Journal of Immunology  1998 160:1204-1211  PMID:9570535
    • Gascoigne, N. R. J. Natural killer cells: Influence of the home environment Current Biology  1997 7:R624-R626  DOI:10.1016/s0960-9822(06)00318-6  PMID:9368742
    • Sim, B. C., Travers, P. J., Gascoigne, N. R. J. V alpha 3.2 selection in mhc class i mutant mice - evidence for an alternate orientation of tcr-mhc class i interaction Journal of Immunology  1997 159:3322-3329  PMID:9317130
    • Wung, J. L., Gascoigne, N. R. J. Selection of phage-displayed superantigen by binding to cell-surface mhc class ii Journal of Immunological Methods  1997 204:33-41  DOI:10.1016/s0022-1759(97)00038-0  PMID:9202707
    • Wung, J. L., Gascoigne, N. R. J. Antibody screening for secreted proteins expressed in pichia pastoris Biotechniques  1996 21:808, 810, 812  PMID:8922617
    • Sim, B. C., Zerva, L., Greene, M. I., Gascoigne, N. R. J. Control of mhc restriction by tcr v-alpha cdr1 and cdr2 Science  1996 273:963-966  DOI:10.1126/science.273.5277.963  PMID:8688082
    • Alam, S. M., Travers, P. J., Wung, J. L., Nasholds, W., Redpath, S., Jameson, S. C., Gascoigne, N. R. J. T-cell-receptor affinity and thymocyte positive selection Nature  1996 381:616-620  DOI:10.1038/381616a0  PMID:8637599
    • Alam, S. M., Crispe, I. N., Gascoigne, N. R. J. Allelic exclusion of mouse t-cell receptor-alpha chains occurs at the time of thymocyte tcr up-regulation Immunity  1995 3:449-458  PMID:7584136
    • Alam, S. M., Sim, B. C., Gascoigne, N. R. J. Selection of tcr v-alpha by mhc class-ii predicts superantigen reactivity International Immunology  1995 7:1311-1318  DOI:10.1093/intimm/7.8.1311  PMID:7495738
    • Dohlsten, M., Abrahmsen, L., Bjork, P., Lando, P. A., Hedlund, G., Forsberg, G., Brodin, T., Gascoigne, N. R. J., Forberg, C., Lind, P., Kalland, T. Monoclonal antibody-superantigen fusion proteins - tumor-specific agents for t-cell-based tumor-therapy Proceedings of the National Academy of Sciences of the United States of America  1994 91:8945-8949  DOI:10.1073/pnas.91.19.8945  PMID:8090750
    • Webb, S. R., Gascoigne, N. R. J. T-cell activation by superantigens Current Opinion in Immunology  1994 6:467-475  DOI:10.1016/0952-7915(94)90129-5  PMID:7917116
    • Irwin, M. J., Gascoigne, N. R. J. Interplay between superantigens and the immune-system Journal of Leukocyte Biology  1993 54:495-503  PMID:8228628
    • Gascoigne, N. R. J. What role does the alpha-chain play in recognition of superantigens Research in Immunology  1993 144:184-187  DOI:10.1016/0923-2494(93)80115-f  PMID:7694343
    • Irwin, M. J., Hudson, K. R., Ames, K. T., Fraser, J. D., Gascoigne, N. R. J. T-cell receptor beta-chain binding to enterotoxin superantigens Immunological Reviews  1993 131:61-78  DOI:10.1111/j.1600-065X.1993.tb01530.x  PMID:8486394
    • Irwin, M. J., Hudson, K. R., Fraser, J. D., Gascoigne, N. R. J. Enterotoxin residues determining t-cell receptor v-beta binding-specificity Nature  1992 359:841-843  DOI:10.1038/359841a0  PMID:1436060
    • Pontzer, C. H., Irwin, M. J., Gascoigne, N. R. J., Johnson, H. M. T-cell antigen receptor-binding sites for the microbial superantigen staphylococcal enterotoxin-a Proceedings of the National Academy of Sciences of the United States of America  1992 89:7727-7731  DOI:10.1073/pnas.89.16.7727  PMID:1502191
    • Nakajima, P. B., Divincenzo, J. P., Jameson, S. C., Gascoigne, N. R. J. Chromosome-14 in b10.A(18r) mice is recombinant and includes tcra-va alleles Immunogenetics  1992 35:190-198  PMID:1371499
    • Gahm, S. J., Fowlkes, B. J., Jameson, S. C., Gascoigne, N. R. J., Cotterman, M. M., Kanagawa, O., Schwartz, R. H., Matis, L. A. Profound alteration in an alpha-beta t-cell antigen receptor repertoire due to polymorphism in the 1st complementarity-determining region of the beta-chain Proceedings of the National Academy of Sciences of the United States of America  1991 88:10267-10271  DOI:10.1073/pnas.88.22.10267  PMID:1835090
    • Jameson, S. C., Nakajima, P. B., Brooks, J. L., Heath, W., Kanagawa, O., Gascoigne, N. R. J. The t-cell receptor v-alpha-11 gene family - analysis of allelic sequence polymorphism and demonstration of j-alpha region-dependent recognition by allele-specific antibodies Journal of Immunology  1991 147:3185-3193  PMID:1717591
    • Gascoigne, N. R. J., Ames, K. T. Direct binding of secreted t-cell receptor beta chain to superantigen associated with class-ii major histocompatibility complex protein Proceedings of the National Academy of Sciences of the United States of America  1991 88:613-616  DOI:10.1073/pnas.88.2.613  PMID:1824876
    • Jameson, S. C., Kaye, J., Gascoigne, N. R. J. A t-cell receptor-v-alpha region selectively expressed in cd4+ cells Journal of Immunology  1990 145:1324-1331  PMID:1696594
    • Gascoigne, N. R. J. Transport and secretion of truncated t-cell receptor beta-chain occurs in the absence of association with cd3 Journal of Biological Chemistry  1990 265:9296-9301  PMID:2140571
    • Kaye, J., Hsu, M. L., Sauron, M. E., Jameson, S. C., Gascoigne, N. R. J., Hedrick, S. M. Selective development of cd4+ t-cells in transgenic mice expressing a class-ii mhc-restricted antigen receptor Nature  1989 341:746-749  DOI:10.1038/341746a0  PMID:2571940
    • Gascoigne, N. R. J., Goodnow, C. C., Dudzik, K. I., Oi, V. T., Davis, M. M. Secretion of a chimeric t-cell receptor immunoglobulin protein Proceedings of the National Academy of Sciences of the United States of America  1987 84:2936-2940  DOI:10.1073/pnas.84.9.2936  PMID:3472243
    • Gascoigne, N. R. J., Waters, S., Elliott, J. F., Victorkobrin, C., Goodnow, C., Davis, M. M., Bona, C. A. Expression of t-cell receptor genes in an antigen-specific hybridoma and radiation-induced variants Journal of Experimental Medicine  1986 164:113-130  DOI:10.1084/jem.164.1.113  PMID:2425026
    • Davis, M. M., Lindsten, T., Gascoigne, N. R. J., Goodnow, C., Chien, Y. H. T-cell receptor gene structure and function Cellular Immunology  1986 99:24-28  DOI:10.1016/0008-8749(86)90211-x  PMID:3489561
    • Davis, M. M., Lindsten, T., Gascoigne, N. R. J., Goodnow, C., Chien, Y. H. Paradigm regained - similarities and differences between t-cell receptor and immunoglobulin genes Biochemical Society Symposium  1986 205-209
    • Hedrick, S. M., Germain, R. N., Bevan, M. J., Dorf, M., Engel, I., Fink, P., Gascoigne, N., Heberkatz, E., Kapp, J., Kaufmann, Y., Kaye, J., Melchers, F., et al. Rearrangement and transcription of a t-cell receptor beta-chain gene in different t-cell subsets Proceedings of the National Academy of Sciences of the United States of America  1985 82:531-535  DOI:10.1073/pnas.82.2.531  PMID:2578667
    • Becker, D. M., Patten, P., Chien, Y. H., Yokota, T., Eshhar, Z., Giedlin, M., Gascoigne, N. R. J., Goodnow, C., Wolf, R., Arai, K., Davis, M. M. Variability and repertoire size of t-cell receptor v-alpha gene segments Nature  1985 317:430-434  DOI:10.1038/317430a0  PMID:2995827
    • Davis, M. M., Chien, Y., Gascoigne, N. R. J., Hedrick, S. M. A murine t-cell receptor gene-complex - isolation, structure and rearrangement Immunological Reviews  1984 81:235-258  DOI:10.1111/j.1600-065X.1984.tb01113.x  PMID:6096259
    • Crispe, I. N., Gascoigne, N. R. J., Owens, T. An allospecific murine T helper clone which can help both T and B cell responses in vitro and in vivo Immunology  1984 52:55-65  PMID:6232208  PMCID:PMC1454605
    • Gascoigne, N. R. J., Crispe, I. N. Differential expression of the repertoire in in vitro and in vivo responses to minor alloantigens Immunogenetics  1984 19:511-517  DOI:10.1007/bf00403442  PMID:6610635
    • Gascoigne, N. R. J., Chien, Y. H., Becker, D. M., Kavaler, J., Davis, M. M. Genomic organization and sequence of t-cell receptor beta-chain constant-region and joining-region genes Nature  1984 310:387-391  DOI:10.1038/310387a0  PMID:6336329
    • Czitrom, A. A., Gascoigne, N. R. J., Edwards, S., Waterfield, D. J. Induction of minor alloantigen-specific T cell subsets in vivo: recognition of processed antigen by helper but not by cytotoxic T cell precursors Journal of Immunology  1984 133:33-39  PMID:6202785
    • Chien, Y. H., Gascoigne, N. R. J., Kavaler, J., Lee, N. E., Davis, M. M. Somatic recombination in a murine t-cell receptor gene Nature  1984 309:322-326  DOI:10.1038/309322a0  PMID:6328306
    • Gascoigne, N. R. J. Suppression of the cytotoxic T cell response to minor alloantigens in vivo. II. Fine specificity of suppressor T cells and lack of restriction by immunoglobulin heavy chain-linked gene products European Journal of Immunology  1984 14:677-680  DOI:10.1002/eji.1830140717  PMID:6235118
    • Gascoigne, N. R. J., Crispe, I. N. Suppression of the cytotoxic T cell response to minor alloantigens in vivo. Linked recognition by suppressor T cells European Journal of Immunology  1984 14:210-215  DOI:10.1002/eji.1830140303  PMID:6200331
    • Gascoigne, N. R. J., Lai, P. K. T helper cell lines that augment in vivo cytotoxic T-cell responses to minor alloantigens Cellular Immunology  1984 83:302-312  DOI:10.1016/0008-8749(84)90309-5  PMID:6198100
    • Owens, T., Czitrom, A. A., Gascoigne, N. R. J., Crispe, I. N., Ratcliffe, M. J. H., Lai, P. K., Mitchison, N. A. The presentation of cell-surface alloantigens to t-cells Immunobiology  1984 168:189-201  PMID:6241598
    • Crispe, I. N., Gascoigne, N. R. J. Computer-assisted analysis of in vivo cytotoxic T cell responses Journal of Immunological Methods  1983 64:91-98  DOI:10.1016/0022-1759(83)90387-3  PMID:6606000
    • Crispe, I. N., Gascoigne, N. R. J., Lai, P. K., Mitchison, N. A., Owens, T. Immunoregulation of the response to membrane alloantigens Transplantation Proceedings  1983 15:685-691
    • Czitrom, A. A., Gascoigne, N. R. J. Primary t-cell responses to minor alloantigens .1. Characterization of cyto-toxic effector-cells generated from regional lymph-nodes after immunization in the footpad Immunology  1983 50:121-129  PMID:6193055  PMCID:PMC1454219
    • Lai, P. K., Waterfield, J. D., Gascoigne, N. R. J., Sharrock, C. E. M., Mitchison, N. A. T-cell responses to minor histocompatibility antigens Immunology  1982 47:371-381  PMID:6214502  PMCID:PMC1555461

featured in

  • Solution to Immune Mystery Could Lead to Better Therapies  News Release
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Research

research overview

  • Molecular Interactions During T Cell Activation

    The interests of my lab are centered on the basis of T cell activation through the T cell receptor during thymocyte development and the immune response. A major aspect of our current work is real-time imaging of molecular interactions during T cell activation, using fluorescent chimeras between cell surface or intracellular proteins and variants of the green fluorescent protein. Live cell deconvolution microscopy allows visualization of movements of these proteins into the immunological synapse during T cell activation, and, using fluorescence resonance energy transfer (FRET) between the fluorescent proteins, we can watch interactions between the proteins. During T cell activation, the T cell receptor must distinguish the few antigenic ligands from a vastly greater number of very similar, but non-stimulatory endogenous ligands. We have found that these non-stimulatory ligands in fact aid in the recognition of the antigenic ligand, and we are now seeking to understand the molecular basis of this phenomenon, as this is critical to understanding the basis of T cell activation. We recently discovered a new T cell specific protein that is necessary for T cell receptor-mediated signal transduction. The thymic positive selection process is defective in the absence of this gene, but the signaling pathway involved seems to be novel. Identifying how this molecule works is a new focus of the lab and is using a wide range of immunological, molecular genetic, biochemical and imaging tools.

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Teaching

advisees

  • graduate advising relationship

    • Yachi, Pia, Ph.D.  candidate, 1999 - 2004
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Background

education and training

  • Ph.D. in Immunology, University of London 1980 - 1983
  • B.Sc. in Zoology, Aberystwyth University 1977 - 1980
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Contact

full name

  • Nicholas R. J. Gascoigne

geographic location

  • Scripps California 
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Identity

ORCID iD

  • ORCID iD http://orcid.org/0000-0001-9980-4225

Scopus ID

  • 7005940463

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