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Cline, Hollis
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Cline, Hollis

Faculty Member
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Positions

  • 2017 - Hahn Professor of Neuroscience, Neuroscience , Scripps Research
  • 2017 - Co-Chair, Neuroscience , Scripps Research
  • 2017 - Professor (Joint Appointment), Molecular Medicine , Scripps Research
  • 2016 - Director, Dorris Neuroscience Center , Scripps Research
  • 2013 - Adjunct Professor, Graduate Program, Salk Institute for Biological Studies
  • 2010 - Professor (Joint Appointment), Dorris Neuroscience Center , Scripps Research
  • 2009 - Adjunct Professor, Department of Neurosciences, School of Medicine. Member, Neurosciences Graduate Program, University of California, San Diego
  • 2008 - Faculty Member, Skaggs Graduate School of Chemical and Biological Sciences , Scripps Research
  • 2016 - 2017 Chairman, Molecular and Cellular Neuroscience (MCN) , Scripps Research
  • 2013 - 2017 Hahn Professor of Neuroscience, Molecular and Cellular Neuroscience (MCN) , Scripps Research
  • 2008 - 2017 Professor (Joint Appointment), Chemical Physiology , Scripps Research
  • 2008 - 2012 Professor, Cell Biology , Scripps Research
  • 2008 - 2012 Hahn Professor of Neuroscience, Cell Biology , Scripps Research
  • 2000 - 2008 Charles and Marie Robertson Professor of Neuroscience, Cold Spring Harbor Laboratory
  • 1998 - 2008 Professor, Cold Spring Harbor Laboratory
  • 2002 - 2006 Director of Research, Cold Spring Harbor Laboratory
  • 1997 - 1998 Associate Professor, Cold Spring Harbor Laboratory
  • 1994 - 1996 Assistant Professor, Cold Spring Harbor Laboratory
  • 1990 - 1993 Assistant Professor, Department of Physiology and Biophysics, University of Iowa
  • 1977 - 1979 Research Assistant, (A. S. Schneider) Department of Endocrinology, Sloan-Kettering Institute
  • 1976 Student Research Assistant, (C. DeDuve), Lab of Biochemical Cytology, The Rockefeller University
Dr. Hollis Cline studies the analysis of the activity-dependent control of cell proliferation, neuronal development and circuit formation in the visual system using gene transfer, in vivo imaging and electrophysiological techniques.

Research Areas research areas

  • Blindness (UMLS)
  • Mental Illness (UMLS)

Contact

  • cline@scripps.edu

Websites

  • Hollis Cline, Ph.D.
  • Laboratory Website
  • Publications
  • Research
  • Teaching
  • Background
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Publications

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  • Publications without PubMed PMIDs

  • academic article

    • Hiramoto, M., Cline, H. T. NMDARs Translate Sequential Temporal Information into Spatial Maps iScience  2020 23  DOI:10.1016/j.isci.2020.101130  PMID:32480133  PMCID:PMC7262552
    • Cline, H. T., Schwarz, T. L. Editorial overview: Cellular Neuroscience Current Opinion in Neurobiology  2020 63:III-VI  DOI:10.1016/j.conb.2020.07.003  PMID:32892945
    • Cline, H., Coolen, L., de Vries, S., Hyman, S., Segal, R., Steward, O. Recognizing Team Science Contributions in Academic Hiring, Promotion, and Tenure Journal of Neuroscience  2020 40:6662-6663  DOI:10.1523/jneurosci.1139-20.2020  PMID:32847999  PMCID:PMC7455215
    • McKeown, C. R., Cline, H. T. Nutrient restriction causes reversible G2 arrest in Xenopus neural progenitors Development  2019 146  DOI:10.1242/dev.178871  PMID:31649012  PMCID:PMC6826041
    • Schiapparelli, L. M., Shah, S. H., Ma, Y., McClatchy, D. B., Sharma, P., Li, J., Yates III, J. R., Goldberg, J. L., Cline, H. T. The retinal ganglion cell transportome identifies proteins transported to axons and presynaptic compartments in the visual system in vivo Cell Reports  2019 28:1935-1947  DOI:10.1016/j.celrep.2019.07.037  PMID:31412257  PMCID:PMC6707540
    • Sharma, P., Mesci, P., Carromeu, C., McClatchy, D. R., Schiapparelli, L., Yates III, J. R., Muotri, A. R., Cline, H. T. Exosomes regulate neurogenesis and circuit assembly Proceedings of the National Academy of Sciences of the United States of America  2019 116:16086-16094  DOI:10.1073/pnas.1902513116  PMID:31320591  PMCID:PMC6689941
    • Gambrill, A. C., Faulkner, R. L., McKeown, C. R., Cline, H. T. Enhanced visual experience rehabilitates the injured brain in Xenopus tadpoles in an NMDAR-dependent manner Journal of Neurophysiology  2019 121:306-320  DOI:10.1152/jn.00664.2018  PMID:30517041
    • He, H. Y., Shen, W., Zheng, L., Guo, X., Cline, H. T. Excitatory synaptic dysfunction cell-autonomously decreases inhibitory inputs and disrupts structural and functional plasticity Nature Communications  2018 9  DOI:10.1038/s41467-018-05125-4  PMID:30042473  PMCID:PMC6057951
    • Gambrill, A. C., Faulkner, R. L., Cline, H. T. Direct intertectal inputs are an integral component of the bilateral sensorimotor circuit for behavior in Xenopus tadpoles Journal of Neurophysiology  2018 119:1947-1961  DOI:10.1152/jn.00051.2018  PMID:29442555  PMCID:PMC6008090
    • Liu, H. H., McClatchy, D. B., Schiapparelli, L., Shen, W. H., Yates III, J. R., Cline, H. T. Role of the visual experience-dependent nascent proteome in neuronal plasticity Elife  2018 7  DOI:10.7554/eLife.33420  PMID:29412139  PMCID:PMC5815848
    • Lissek, T., Adams, M., Adelman, J., Ahissar, E., Akaaboune, M., Akil, H., al'Absi, M., Arain, F., Arango-Lasprilla, J. C., Atasoy, D., Avila, J., Badawi, A., et al. Building Bridges through Science Neuron  2017 96:730-735  DOI:10.1016/j.neuron.2017.09.028  PMID:29144972
    • McKeown, C. R., Thompson, C. K., Cline, H. T. Reversible developmental stasis in response to nutrient availability in the Xenopus laevis central nervous system Journal of Experimental Biology  2017 220:358-368  DOI:10.1242/jeb.151043  PMID:27875263  PMCID:PMC5312736
    • Gambrill, A. C., Faulkner, R. L., Cline, H. T. Experience-dependent plasticity of excitatory and inhibitory intertectal inputs in Xenopus tadpoles Journal of Neurophysiology  2016 116:2281-2297  DOI:10.1152/jn.00611.2016  PMID:27582296  PMCID:PMC5110636
    • Pratt, K. G., Hiramoto, M., Cline, H. T. An evolutionarily conserved mechanism for activity-dependent visual circuit development Frontiers in Neural Circuits  2016 10  DOI:10.3389/fncir.2016.00079  PMID:27818623  PMCID:PMC5073143
    • Thompson, C. K., Cline, H. T. Thyroid hormone acts locally to increase neurogenesis, neuronal differentiation, and dendritic arbor elaboration in the tadpole visual system Journal of Neuroscience  2016 36:10356-10375  DOI:10.1523/jneurosci.4147-15.2016  PMID:27707971  PMCID:PMC5050329
    • Truszkowski, T. L. S., James, E. J., Hasan, M., Wishard, T. J., Liu, Z., Pratt, K. G., Cline, H. T., Aizenman, C. D. Fragile X mental retardation protein knockdown in the developing Xenopus tadpole optic tectum results in enhanced feedforward inhibition and behavioral deficits Neural Development  2016 11  DOI:10.1186/s13064-016-0069-7  PMID:27503008  PMCID:PMC4977860
    • Liu, H. H., Cline, H. T. Fragile X mental retardation protein is required to maintain visual conditioning-induced behavioral plasticity by limiting local protein synthesis Journal of Neuroscience  2016 36:7325-7339  DOI:10.1523/jneurosci.4282-15.2016  PMID:27383604  PMCID:PMC4938868
    • He, H. Y., Shen, W., Hiramoto, M., Cline, H. T. Experience-dependent bimodal plasticity of inhibitory neurons in early development Neuron  2016 90:1203-1214  DOI:10.1016/j.neuron.2016.04.044  PMID:27238867  PMCID:PMC4938159
    • Bestman, J. E., Huang, L. C., Lee-Osbourne, J., Cheung, P., Cline, H. T. An in vivo screen to identify candidate neurogenic genes in the developing Xenopus visual system Developmental Biology  2015 408:269-291  DOI:10.1016/j.ydbio.2015.03.010  PMID:25818835  PMCID:PMC4584193
    • Muñoz, R., Edwards-Faret, G., Moreno, M., Zuñiga, N., Cline, H., Larrain, J. Regeneration of Xenopus laevis spinal cord requires Sox2/3 expressing cells Developmental Biology  2015 408:229-243  DOI:10.1016/j.ydbio.2015.03.009  PMID:25797152  PMCID:PMC4826040
    • Faulkner, R. L., Wishard, T. J., Thompson, C. K., Liu, H. H., Cline, H. T. FMRP regulates neurogenesis in vivo in Xenopus laevis tadpoles eNeuro  2015 2  DOI:10.1523/eneuro.0055-14.2014  PMID:25844398  PMCID:PMC4384423
    • Hiramoto, M., Cline, H. T. Optic flow instructs retinotopic map formation through a spatial to temporal to spatial transformation of visual information Proceedings of the National Academy of Sciences of the United States of America  2014 111:E5105-E5113  DOI:10.1073/pnas.1416953111  PMID:25385606  PMCID:PMC4250144
    • Schiapparelli, L. M., McClatchy, D. B., Liu, H. H., Sharma, P., Yates III, J. R., Cline, H. T. Direct detection of biotinylated proteins by mass spectrometry Journal of Proteome Research  2014 13:3966-3978  DOI:10.1021/pr5002862  PMID:25117199  PMCID:PMC4156236
    • Shen, W., Liu, H. H., Schiapparelli, L., McClatchy, D., He, H. Y., Yates III, J. R., Cline, H. T. Acute synthesis of CPEB is required for plasticity of visual avoidance behavior in Xenopus Cell Reports  2014 6:737-747  DOI:10.1016/j.celrep.2014.01.024  PMID:24529705  PMCID:PMC3962200
    • Bestman, J. E., Cline, H. T. Morpholino studies in Xenopus brain development Methods in Molecular Biology  2014 1082:155-171  DOI:10.1007/978-1-62703-655-9_11  PMID:24048933
    • Sharma, P., Schiapparelli, L., Cline, H. T. Exosomes function in cell-cell communication during brain circuit development Current Opinion in Neurobiology  2013 23:997-1004  DOI:10.1016/j.conb.2013.08.005  PMID:23998929  PMCID:PMC3830597
    • McKeown, C. R., Sharma, P., Sharipov, H. E., Shen, W. H., Cline, H. T. Neurogenesis is required for behavioral recovery after injury in the visual system of Xenopus laevis Journal of Comparative Neurology  2013 521:2262-2278  DOI:10.1002/cne.23283  PMID:23238877  PMCID:PMC3626762
    • Lee, P. C., He, H. Y., Lin, C. Y., Ching, Y. T., Cline, H. T. Computer aided alignment and quantitative 4D structural plasticity analysis of neurons Neuroinformatics  2013 11:249-257  DOI:10.1007/s12021-013-9179-0  PMID:23408326  PMCID:PMC3622159
    • Demas, J. A., Payne, H., Cline, H. T. Vision drives correlated activity without patterned spontaneous activity in developing Xenopus retina Developmental Neurobiology  2012 72:537-546  DOI:10.1002/dneu.20880  PMID:21312343  PMCID:PMC3157589
    • Bestman, J. E., Lee-Osbourne, J., Cline, H. T. In vivo time-lapse imaging of cell proliferation and differentiation in the optic tectum of Xenopus laevis tadpoles Journal of Comparative Neurology  2012 520:401-433  DOI:10.1002/cne.22795  PMID:22113462  PMCID:PMC3366109
    • Shen, W. H., McKeown, C. R., Demas, J. A., Cline, H. T. Inhibition to excitation ratio regulates visual system responses and behavior in vivo Journal of Neurophysiology  2011 106:2285-2302  DOI:10.1152/jn.00641.2011  PMID:21795628  PMCID:PMC3214097
    • Hiramoto, M., Cline, H. T. Mapping dynamic branch displacements: A versatile method to quantify spatiotemporal neurite dynamics Frontiers in Neural Circuits  2011 5:13  DOI:10.3389/fncir.2011.00013  PMID:21994492  PMCID:PMC3183586
    • He, H. Y., Cline, H. T. Diadem X: Automated 4 dimensional analysis of morphological data Neuroinformatics  2011 9:107-112  DOI:10.1007/s12021-011-9098-x  PMID:21271361  PMCID:PMC3104138
    • Li, J. L., Erisir, A., Cline, H. In vivo time-lapse imaging and serial section electron microscopy reveal developmental synaptic rearrangements Neuron  2011 69:273-286  DOI:10.1016/j.neuron.2010.12.022  PMID:21262466  PMCID:PMC3052740
    • Sharma, P., Cline, H. T. Visual activity regulates neural progenitor cells in developing xenopus CNS through musashi1 Neuron  2010 68:442-455  DOI:10.1016/j.neuron.2010.09.028  PMID:21040846  PMCID:PMC3005332
    • Li, J. L., Cline, H. T. Visual deprivation increases accumulation of dense core vesicles in developing optic tectal synapses in Xenopus laevis Journal of Comparative Neurology  2010 518:2365-2381  DOI:10.1002/cne.22338  PMID:20437533  PMCID:PMC2980367
    • Chiu, S. L., Cline, H. T. Insulin receptor signaling in the development of neuronal structure and function Neural Development  2010 5:7  DOI:10.1186/1749-8104-5-7  PMID:20230616  PMCID:PMC2843688
    • Li, J. L., Wang, Y., Chiu, S. L., Cline, H. T. Membrane targeted horseradish peroxidase as a marker for correlative fluorescence and electron microscopy studies Frontiers in Neural Circuits  2010 4:6  DOI:10.3389/neuro.04.006.2010  PMID:20204144  PMCID:PMC2831632
    • Wurdak, H., Zhu, S. T., Min, K. H., Aimone, L., Lairson, L. L., Watson, J., Chopiuk, G., Demas, J., Charette, B., Weerapana, E., Cravatt, B. F., Cline, H. T. A small molecule accelerates neuronal differentiation in the adult rat Proceedings of the National Academy of Sciences of the United States of America  2010 107:16542-16547  DOI:10.1073/pnas.1010300107  PMID:20823227  PMCID:PMC2944756
    • Hiramoto, M., Cline, H. T. Convergence of multisensory inputs in Xenopus tadpole tectum Developmental Neurobiology  2009 69:959-971  DOI:10.1002/dneu.20754  PMID:19813244  PMCID:PMC2902241
    • Shen, W. H., Da Silva, J. S., He, H. Y., Cline, H. T. Type A GABA-receptor-dependent synaptic transmission sculpts dendritic arbor structure in Xenopus tadpoles in vivo Journal of Neuroscience  2009 29:5032-5043  DOI:10.1523/jneurosci.5331-08.2009  PMID:19369572  PMCID:PMC2706946
    • Bohland, J. W., Wu, C. Z., Barbas, H., Bokil, H., Bota, M., Breiter, H. C., Cline, H. T., Doyle, J. C., Freed, P. J., Greenspan, R. J., Haber, S. N., Hawrylycz, M., et al. A proposal for a coordinated effort for the determination of brainwide neuroanatomical connectivity in model organisms at a mesoscopic scale PLoS Computational Biology  2009 5:e1000334  DOI:10.1371/journal.pcbi.1000334  PMID:19325892  PMCID:PMC2655718
    • Bestman, J. E., Cline, H. T. The RNA binding protein CPEB regulates dendrite morphogenesis and neuronal circuit assembly in vivo Proceedings of the National Academy of Sciences of the United States of America  2008 105:20494-20499  DOI:10.1073/pnas.0806296105  PMID:19074264  PMCID:PMC2629308
    • Van Keuren-Jensen, K. R., Cline, H. T. Homer proteins shape Xenopus optic tectal cell dendritic arbor development in vivo Developmental Neurobiology  2008 68:1315-1324  DOI:10.1002/dneu.20659  PMID:18636533  PMCID:PMC3641002
    • Thirumalai, V., Cline, H. T. Endogenous dopamine suppresses initiation of swimming in prefeeding zebrafish larvae Journal of Neurophysiology  2008 100:1635-1648  DOI:10.1152/jn.90568.2008  PMID:18562547  PMCID:PMC2544474
    • Chiu, S. L., Chen, C. M., Cline, H. T. Insulin receptor signaling regulates synapse number, dendritic plasticity, and circuit function in vivo Neuron  2008 58:708-719  DOI:10.1016/j.neuron.2008.04.014  PMID:18549783  PMCID:PMC3057650
    • Cantallops, I., Cline, H. T. Rapid activity-dependent neurotrophic protein CPG15 delivery of the to the axon surface of neurons in intact Xenopus tadpoles Developmental Neurobiology  2008 68:744-759  DOI:10.1002/dneu.20529  PMID:18383547
    • Cline, H., Haas, K. The regulation of dendritic arbor development and plasticity by glutamatergic synaptic input: A review of the synaptotrophic hypothesis Journal of Physiology-London  2008 586:1509-1517  DOI:10.1113/jphysiol.2007.150029  PMID:18202093  PMCID:PMC2375708
    • Ewald, R. C., Van Keuren-Jensen, K. R., Aizenman, C. D., Cline, H. T. Roles of NR2A and NR2B in the development of dendritic arbor morphology in vivo Journal of Neuroscience  2008 28:850-861  DOI:10.1523/jneurosci.5078-07.2008  PMID:18216193
    • Akerman, C. J., Cline, H. T. Refining the roles of GABAergic signaling during neural circuit formation Trends in Neurosciences  2007 30:382-389  DOI:10.1016/j.tins.2007.06.002  PMID:17590449
    • Aizenman, C. D., Cline, H. T. Enhanced visual activity in vivo forms nascent synapses in the developing retinotectal projection Journal of Neurophysiology  2007 97:2949-2957  DOI:10.1152/jn.00452.2006  PMID:17267761
    • Verde, E. M. R., Lee-Osbourne, J., Worley, P. F., Malinow, R., Cline, H. T. Increased expression of the immediate-early gene arc/arg3.1 reduces AMPA receptor-mediated synaptic transmission Neuron  2006 52:461-474  DOI:10.1016/j.neuron.2006.09.031  PMID:17088212  PMCID:PMC3951199
    • Haas, K., Li, J. L., Cline, H. T. Ampa receptors regulate experience-dependent dendritic arbor growth in vivo Proceedings of the National Academy of Sciences of the United States of America  2006 103:12127-12131  DOI:10.1073/pnas.0602670103  PMID:16882725  PMCID:PMC1525049
    • Van Keuren-Jensen, K., Cline, H. T. Visual experience regulates metabotropic glutamate receptor-mediated plasticity of AMPA receptor synaptic transmission by homer1a induction Journal of Neuroscience  2006 26:7575-7580  DOI:10.1523/jneurosci.5083-05.2006  PMID:16855085
    • Akerman, C. J., Cline, H. T. Depolarizing GABAergic conductances regulate the balance of excitation to inhibition in the developing retinotectal circuit in vivo Journal of Neuroscience  2006 26:5117-5130  DOI:10.1523/jneurosci.0319-06.2006  PMID:16687503
    • Ruthazer, E. S., Li, J. L., Cline, H. T. Stabilization of axon branch dynamics by synaptic maturation Journal of Neuroscience  2006 26:3594-3603  DOI:10.1523/jneurosci.0069-06.2006  PMID:16571768
    • Bestman, J. E., Ewald, R. C., Chiu, S. L., Cline, H. T. In vivo single-cell electroporation for transfer of DNA and macromolecules Nature Protocols  2006 1:1267-1272  DOI:10.1038/nprot.2006.186  PMID:17406410
    • Foa, L., Jensen, K., Rajan, I., Bronson, K., Gasperini, R., Worley, P. F., Tu, J. C., Cline, H. T. Homer expression in the Xenopus tadpole nervous system Journal of Comparative Neurology  2005 487:42-53  DOI:10.1002/cne.20496  PMID:15861458
    • Javaherian, A., Cline, H. T. Coordinated motor neuron axon growth and neuromuscular synaptogenesis are promoted by CPG15 in vivo Neuron  2005 45:505-512  DOI:10.1016/j.neuron.2004.12.051  PMID:15721237
    • Van Aelst, L., Cline, H. T. Rho GTPases and activity-dependent dendtite development Current Opinion in Neurobiology  2004 14:297-304  DOI:10.1016/j.conb.2004.05.012  PMID:15194109
    • Ruthazer, E. S., Cline, H. T. Insights into activity-dependent map formation from the retinotectal system: A middle-of-the-brain perspective Journal of Neurobiology  2004 59:134-146  DOI:10.1002/neu.10344  PMID:15007832
    • Aizenman, C. D., Akerman, C. J., Jensen, K. R., Cline, H. T. Visually driven regulation of intrinsic neuronal excitability improves stimulus detection in vivo Neuron  2003 39:831-842  DOI:10.1016/s0896-6273(03)00527-0  PMID:12948449
    • Ruthazer, E. S., Akerman, C. J., Cline, H. T. Control of axon branch dynamics by correlated activity in vivo Science  2003 301:66-70  DOI:10.1126/science.1082545  PMID:12843386
    • Wu, G. Y., Cline, H. T. Time-lapse in vivo imaging of the morphological development of Xenopus optic tectal interneurons Journal of Comparative Neurology  2003 459:392-406  DOI:10.1002/cne.10618  PMID:12687706
    • Sin, W. C., Haas, K., Ruthazer, E. S., Cline, H. T. Dendrite growth increased by visual activity requires NMDA receptor and Rho GTPases Nature  2002 419:475-480  DOI:10.1038/nature00987  PMID:12368855
    • Ruthazer, E. S., Cline, H. T. Multiphoton imaging of neurons in living tissue: Acquisition and analysis of time-lapse morphological data Real-Time Imaging  2002 8:175-188  DOI:10.1006/rtim.2002.0284
    • Haas, K., Jensen, K., Sin, W. C., Foa, L., Cline, H. T. Targeted electroporation in Xenopus tadpoles in vivo - from single cells to the entire brain Differentiation  2002 70:148-154  DOI:10.1046/j.1432-0436.2002.700404.x  PMID:12147134
    • Aizenman, C. D., Munoz-Elias, G., Cline, H. T. Visually driven modulation of glutamatergic synaptic transmission is mediated by the regulation of intracellular polyamines Neuron  2002 34:623-634  DOI:10.1016/s0896-6273(02)00674-8  PMID:12062045
    • Lisman, J., Schulman, H., Cline, H. The molecular basis of CaMKII function in synaptic and behavioural memory Nature Reviews Neuroscience  2002 3:175-190  DOI:10.1038/nrn753  PMID:11994750
    • Li, Z., Aizenman, C. D., Cline, H. T. Regulation of Rho GTPases by crosstalk and neuronal activity in vivo Neuron  2002 33:741-750  DOI:10.1016/s0896-6273(02)00621-9  PMID:11879651
    • Debski, E. A., Cline, H. T. Activity-dependent mapping in the retinotectal projection Current Opinion in Neurobiology  2002 12:93-99  DOI:10.1016/s0959-4388(02)00295-7  PMID:11861170
    • Peunova, N., Scheinker, V., Cline, H., Enikolopov, G. Nitric oxide is an essential negative regulator of cell proliferation in Xenopus brain Journal of Neuroscience  2001 21:8809-8818  PMID:11698593
    • Nedivi, E., Javaherian, A., Cantallops, I., Cline, H. T. Developmental regulation of CPG15 expression in Xenopus Journal of Comparative Neurology  2001 435:464-473  DOI:10.1002/cne.1044  PMID:11406826
    • Foa, L., Rajan, I., Haas, K., Wu, G. Y., Brakeman, P., Worley, P., Cline, H. The scaffold protein, Homer1b/c, regulates axon pathfinding in the central nervous system in vivo Nature Neuroscience  2001 4:499-506  PMID:11319558
    • Dickson, B. J., Cline, H., Polleux, F., Ghosh, A. Making connections - meeting: Axon guidance and neural plasticity EMBO Reports  2001 2:182-186  DOI:10.1093/embo-reports/kve051  PMID:11266357  PMCID:PMC1083847
    • Haas, K., Sin, W. C., Javaherian, A., Li, Z., Cline, H. T. Single-cell electroporation for gene transfer in vivo Neuron  2001 29:583-591  DOI:10.1016/s0896-6273(01)00235-5  PMID:11301019
    • Cline, H. T. Dendritic arbor development and synaptogenesis Current Opinion in Neurobiology  2001 11:118-126  DOI:10.1016/s0959-4388(00)00182-3  PMID:11179881
    • Cantallops, I., Haas, K., Cline, H. T. Postsynaptic CPG15 promotes synaptic maturation and presynaptic axon arbor elaboration in vivo Nature Neuroscience  2000 3:1004-1011  PMID:11017173
    • Cantallops, I., Cline, H. T. Synapse formation: If it looks like a duck and quacks like a duck Current Biology  2000 10:R620-R623  DOI:10.1016/s0960-9822(00)00663-1  PMID:10996085
    • Li, Z., Van Aelst, L., Cline, H. T. Rho GTPases regulate distinct aspects of dendritic arbor growth in Xenopus central neurons in vivo Nature Neuroscience  2000 3:217-225  PMID:10700252
    • Kay, A. R., Alfonso, A., Alford, S., Cline, H. T., Holgado, A. M., Sakmann, B., Snitsarev, V. A., Stricker, T. P., Takahashi, M., Wu, L. G. Imaging synaptic activity in intact brain and slices with FM1-43 in C-elegans, lamprey, and rat Neuron  1999 24:809-817  DOI:10.1016/s0896-6273(00)81029-6  PMID:10624945
    • Zou, D. J., Cline, H. T. Postsynaptic calcium/calmodulin-dependent protein kinase II is required to limit elaboration of presynaptic and postsynaptic neuronal arbors Journal of Neuroscience  1999 19:8909-8918  PMID:10516310
    • Wu, G. Y., Zou, D. J., Rajan, I., Cline, H. Dendritic dynamics in vivo change during neuronal maturation Journal of Neuroscience  1999 19:4472-4483  PMID:10341248
    • Rajan, I., Witte, S., Cline, H. T. NMDA receptor activity stabilizes presynaptic retinotectal axons and postsynaptic optic tectal cell dendrites in vivo Journal of Neurobiology  1999 38:357-368  DOI:10.1002/(sici)1097-4695(19990215)38:3<357::aid-neu5>3.0.co;2-#  PMID:10022578
    • Edwards, J. A., Cline, H. T. Light-induced calcium influx into retinal axons is regulated by presynaptic nicotinic acetylcholine receptor activity in vivo Journal of Neurophysiology  1999 81:895-907  PMID:10036287
    • Cline, H. Activity-dependent control of dendritic growth in vivo Developmental Neuropsychology  1999 16:331-333  DOI:10.1207/s15326942dn1603_8
    • Cline, H. T. Topographic maps: Developing roles of synaptic plasticity Current Biology  1998 8:R836-R839  DOI:10.1016/s0960-9822(07)00525-8  PMID:9822571
    • Rajan, I., Cline, H. T. Glutamate receptor activity is required for normal development of tectal cells dendrites in vivo Journal of Neuroscience  1998 18:7836-7846  PMID:9742152
    • Haas, K., Cline, H., Malinow, R. No change in NMDA receptor-mediated response rise-time during development: Evidence against transmitter spillover Neuropharmacology  1998 37:1393-1398  DOI:10.1016/s0028-3908(98)00137-3  PMID:9849674
    • Nedivi, E., Wu, G. Y., Cline, H. T. Promotion of dendritic growth by CPG15, an activity-induced signaling molecule Science  1998 281:1863-1866  DOI:10.1126/science.281.5384.1863  PMID:9743502
    • Pinches, E. M., Cline, H. T. Distribution of synaptic vesicle proteins within single retinotectal axons of Xenopus tadpoles Journal of Neurobiology  1998 35:426-434  DOI:10.1002/(sici)1097-4695(19980615)35:4<426::aid-neu8>3.3.co;2-o  PMID:9624623
    • Constantine-Paton, M., Cline, H. T. LTP and activity-dependent synaptogenesis: The more alike they are, the more different they become Current Opinion in Neurobiology  1998 8:139-148  DOI:10.1016/s0959-4388(98)80017-2  PMID:9568401
    • Wu, G. Y., Cline, H. T. Stabilization of dendritic arbor structure in vivo by CaMKII Science  1998 279:222-226  DOI:10.1126/science.279.5348.222  PMID:9422694
    • Jones, K. W., Berry, W. J., Borsay, D. J., Cline, H. T., Conner, W. C., Fullmer, C. S. Applications of synchrotron radiation-induced x-ray emission (srixe) X-Ray Spectrometry  1997 26:350-358  DOI:10.1002/(sici)1097-4539(199711/12)26:6<350::aid-xrs233>3.3.co;2-5
    • Wu, G. Y., Malinow, R., Cline, H. T. Maturation of a central glutamatergic synapse Science  1996 274:972-976  DOI:10.1126/science.274.5289.972  PMID:8875937
    • Witte, S., Stier, H., Cline, H. T. In vivo observations of timecourse and distribution of morphological dynamics in Xenopus retinotectal axon arbors Journal of Neurobiology  1996 31:219-234  DOI:10.1002/(sici)1097-4695(199610)31:2<219::aid-neu7>3.0.co;2-e  PMID:8885202
    • Cline, H. T., Witte, S., Jones, K. W. Low lead levels stunt neuronal growth in a reversible manner Proceedings of the National Academy of Sciences of the United States of America  1996 93:9915-9920  DOI:10.1073/pnas.93.18.9915  PMID:8790431
    • Cline, H. Can there be growth without growth cones? Seminars in the Neurosciences  1996 8:89-95  DOI:10.1006/smns.1996.0012
    • Zou, D. J., Cline, H. T. Expression of constitutively active CaMKII in target tissue modifies presynaptic axon arbor growth Neuron  1996 16:529-539  DOI:10.1016/s0896-6273(00)80072-0  PMID:8785050
    • Cline, H. T., Wu, G. Y., Malinow, R. In vivo development of neuronal structure and function Cold Spring Harbor Symposia on Quantitative Biology  1996 61:95-104  PMID:9246439
    • Wu, G. Y., Zou, D. J., Koothan, T., Cline, H. T. Infection of frog neurons with vaccinia virus permits in vivo expression of foreign proteins Neuron  1995 14:681-684  DOI:10.1016/0896-6273(95)90211-2  PMID:7718230
    • Cline, H. T., McDonald, J. W., Constantinepaton, M. Glutamate-receptor binding in juvenile and adult rana-pipiens cns Journal of Neurobiology  1994 25:488-502  DOI:10.1002/neu.480250504  PMID:8071657
    • Orourke, N. A., Cline, H. T., Fraser, S. E. Rapid remodeling of retinal arbors in the tectum with and without blockade of synaptic transmission Neuron  1994 12:921-934  DOI:10.1016/0896-6273(94)90343-3  PMID:8161460
    • Cline, H. T. Activity-dependent plasticity in the visual systems of frogs and fish Trends in Neurosciences  1991 14:104-111  DOI:10.1016/0166-2236(91)90071-2  PMID:1709534
    • Cline, H. T., Tsien, R. W. Glutamate-induced increases in intracellular Ca2+ in cultured frog tectal cells mediated by direct activation of NMDA receptor channels Neuron  1991 6:259-267  DOI:10.1016/0896-6273(91)90361-3  PMID:1704244
    • Cline, H. T., Constantinepaton, M. The differential influence of protein-kinase inhibitors on retinal arbor morphology and eye-specific stripes in the frog retinotectal system Neuron  1990 4:899-908  DOI:10.1016/0896-6273(90)90143-4  PMID:2361013
    • Cline, H. T., Constantinepaton, M. Nmda receptor agonist and antagonists alter retinal ganglion-cell arbor structure in the developing frog retinotectal projection Journal of Neuroscience  1990 10:1197-1216  PMID:2158526
    • Debski, E. A., Cline, H. T., Constantinepaton, M. Activity-dependent tuning and the NMDA receptor Journal of Neurobiology  1990 21:18-32  DOI:10.1002/neu.480210103  PMID:2156953
    • Constantinepaton, M., Cline, H. T., Debski, E. Patterned activity, synaptic convergence, and the NMDA receptor in developing visual pathways Annual Review of Neuroscience  1990 13:129-154  DOI:10.1146/annurev.neuro.13.1.129  PMID:2183671
    • Cline, H. T., Constantinepaton, M. NMDA receptor antagonists disrupt the retinotectal topographic map Neuron  1989 3:413-426  DOI:10.1016/0896-6273(89)90201-8  PMID:2577128
    • McDonald, J. W., Cline, H. T., Constantinepaton, M., Maragos, W. F., Johnston, M. V., Young, A. B. Quantitative autoradiographic localization of NMDA, quisqualate and PCP receptors in the frog tectum Brain Research  1989 482:155-158  DOI:10.1016/0006-8993(89)90553-2  PMID:2539881
    • Cline, H. T., Debski, E. A., Constantinepaton, M. N-methyl-d-aspartate receptor antagonist desegregates eye-specific stripes Proceedings of the National Academy of Sciences of the United States of America  1987 84:4342-4345  DOI:10.1073/pnas.84.12.4342  PMID:2884663
    • Glover, J. C., Stuart, D. K., Cline, H. T., McCaman, R. E., Magill, C., Stent, G. S. Development of neurotransmitter metabolism in embryos of the leech Haementeria-ghilianii Journal of Neuroscience  1987 7:581-594  PMID:2880941
    • Cline, H. T. Evidence for GABA as a neurotransmitter in the leech Journal of Neuroscience  1986 6:2848-2856  PMID:2876064
    • Cline, H. T., Nusbaum, M. P., Kristan, W. B. Identified GABAergic inhibitory motor neurons in the leech central nervous system take up GABA. Brain Research  1985 348:359-362  DOI:10.1016/0006-8993(85)90457-3  PMID:4075094
    • Cline, H. T. 3H-GABA uptake selectively labels identifiable neurons in the leech central nervous-system Journal of Comparative Neurology  1983 215:351-358  DOI:10.1002/cne.902150309  PMID:6853778
  • thesis

    • Cline, H. CABA-ergic neurons in the leech central nervous system 1985

featured in

  • Pioneering Neuroscientist Hollis T. Cline Joins The Scripps Research Institute  News Release
  • Scripps Research Institute Dedicates Dorris Neuroscience Center  News Release
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Research

research overview

  • Understanding How Experience Controls Brain Development

    My laboratory is interested in the role of sensory experience in controlling the development, function and plasticity of the visual circuit and how circuit development and function become disrupted in neurodevelopmental disorders. We conduct state of the art experiments in visual circuit development using molecular genetic manipulations, electrophysiological methods, in vivo time-lapse imaging, electron microscopy and behavior. Over the past 20 years we have demonstrated roles for a variety of activity-dependent mechanisms in controlling structural plasticity of neuronal dendrites and axons, synaptic maturation and topographic map formation. This body of work has helped to generate a comprehensive understanding of the role of experience in shaping brain development. Two key points to emerge from our research is that circuit formation in vivo is a dynamic process throughout development that is continuously guided by experience, and that the basic mechanisms governing brain development, plasticity, information processing and organizational principles of brain circuits are highly conserved across vertebrates. More recently we have begun to address the role of activity-dependent mechanisms in the development of the functional visual circuit, assessed by in vivo recordings of visual responses in intact animals and behavioral assays. This series of studies has revealed critical molecular/genetic players required for the integration of neurons into functional circuits in vivo.

    Research Projects

    The Dynamic Connectome

    A thorough understanding of circuit development and brain function requires knowledge of the connectivity of brain networks. In vivo time-lapse imaging of dendritic and axonal structures have shown that they are dynamic over the timecourse of hours and days. Importantly the structural dynamics are the physical substrate of synaptic dynamics and therefore dynamics in the connectivity map. We have begun to assess the dynamics in the connectivity map of the optic tectum by combining in vivo time lapse imaging with serial section electron microscopy and three-dimensional reconstruction of optic tectal neuronal dendrites and axons. We use 2 photon time lapse imaging of GFP expressing neurons in living animals to identify dynamic branches within neurons and serial section electron microscopy (EM) to generate 3 dimensional reconstructions of labeled neurons and their synaptic partners. By comparing the live imaging data and the serial section EM data, we determine the synaptic connectivity and ultrastructural synaptic features of dynamics and stable dendritic and axonal branches. This type of analysis will allow us to identify stable and dynamic components of the connectome and to determine how connectivity changes with experience and under conditions that model human neurodevelopemental diseases.

    Regulation of Neurogenesis

    The development of brain networks depends of the spatial and temporal control of cell proliferation and differentiation. We have recently begun several projects to investigate the control of neurogenesis in the tadpole brain. A significant advantage of studying neurogenesis in tadpoles is that the animals develop externally so the entire process from progenitor cell proliferation to neuronal differentiation and integration into brain circuits can be easily visualized and manipulated in the intact animal. We have recently found that visual experience controls neurogenesis and we have conducted microarray analysis to identify candidate molecular genetic pathways through which sensory experience controls neurogenesis.

    Balanced Inhibition in Visual Circuit Function and Behavior

    The retinotectal system processes and integrates visual and mechanosensory information and controls behavioral responses to sensory inputs. The interaction between excitatory and inhibitory synaptic inputs is essential for these brain functions. Excitatory synaptic activity is balanced by inhibitory synaptic input throughout the CNS. Despite the widespread expectation that balanced inhibition to excitation is essential for circuit function and information processing, the consequences of manipulating the ratio of inhibition to excitation on information processing and behavior have not been directly tested. We are using in vivo imaging, electrophysiological methods and behavior combined with molecular genetic manipulations to determine the function of inhibition in visual system function. These projects will the mechanisms controlling the development of inhibitory and excitatory neurons and explore the consequences of disrupting the balance of inhibition to excitation in the intact brain, akin to deficits in information processing seen in neurodevelopmental disorders such as autism spectrum disorders.

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Teaching

advisees

  • graduate advising relationship

    • Verduzco Espinoza, Ana Paola, Ph.D.  candidate, 2019 -
    • Xie, Yi, Ph.D.  candidate, 2019 -
    • Liu, Han-Hsuan, Ph.D.  candidate, 2012 - 2017
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Background

education and training

  • B.A. in Biology, Bryn Mawr College
  • Ph.D. in Neurobiology, University of California, Berkeley , with Dr. Gunther Stent 1985

awards and honors

  • Outstanding Mentor Award, conferred by Scripps Research, 2013
  • Fellow of the American Academy of Arts and Sciences, conferred by American Academy of Arts & Sciences, 2012
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Contact

full name

  • Hollis Tremaine Cline, Ph.D.

geographic location

  • Scripps California 

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