head of
- Patapoutian Laboratory Professor 2000 -
Patapoutian, Ardem Faculty Member
Positions
- Professor, Patapoutian Laboratory, Molecular and Cellular Neuroscience 2000 -
- Faculty Member, Kellogg School of Science and Technology, The Scripps Research Institute 2000 -
- Adjunct Professor, Graduate Program in Neurosciences, University of California, San Diego 2001 -
- Professor, Dorris Neuroscience Center, The Scripps Research Institute 2010 -
- Director of Discovery Research, Genomics Institute of the Novartis Research Foundation 2006 -
- Professor, Molecular and Cellular Neuroscience, The Scripps Research Institute 2013 -
- 2013 HHMI Investigator, Howard Hughes Medical Institute 2013 -
- Professor, Cell Biology, The Scripps Research Institute 2008 - 2012
- Associate Professor, Cell Biology, The Scripps Research Institute 2005 - 2008
- Head of Neuroscience, Genomics Institute of the Novartis Research Foundation 2002 - 2005
- Assistant Professor, Cell Biology, The Scripps Research Institute 2000 - 2005
- Staff Scientist, Genomics Institute of the Novartis Research Foundation 2000 - 2003
- Postdoctoral Fellow with Dr. Louis Reichardt, University of California, San Francisco 1996 - 2000
Dr. Ardem Patapoutian studies the molecular basis of the sense of touch by using genomics, imaging, and transgenic technologies to identify and characterize ion channel proteins involved in the perception of distinct thermal, mechanical, and chemical stimuli.
Affiliation
Publications
selected publications
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academic article
- Coste, B., Patapoutian, A. Neuroscience: Salty sensations Nature 2013 494:44-45 DOI:10.1038/nature11946 PMID:23364688
- Kim, S. E., Patapoutian, A., Grandl, J. Single residues in the outer pore of TRPV1 and TRPV3 have temperature-dependent conformations PLoS One 2013 8:e59593 DOI:10.1371/journal.pone.0059593 PMID:23555720
- Dubin, A. E., Schmidt, M., Mathur, J., Petrus, M. J., Xiao, B. L., Coste, B., Patapoutian, A. Inflammatory signals enhance piezo2-mediated mechanosensitive currents Cell Reports 2012 2:511-517 DOI:10.1016/j.celrep.2012.07.014 PMID:22921401
- Coste, B., Xiao, B. L., Santos, J. S., Syeda, R., Grandl, J., Spencer, K. S., Kim, S. E., Schmidt, M., Mathur, J., Dubin, A. E., Montal, M., Patapoutian, A. Piezo proteins are pore-forming subunits of mechanically activated channels Nature 2012 483:176-181 DOI:10.1038/nature10812 PMID:22343900
- Kim, S. E., Coste, B., Chadha, A., Cook, B., Patapoutian, A. The role of Drosophila Piezo in mechanical nociception Nature 2012 483:209-212 DOI:10.1038/nature10801 PMID:22343891
- Blasius, A. L., Dubin, A. E., Petrus, M. J., Lim, B. K., Narezkina, A., Criado, J. R., Wills, D. N., Xia, Y., Moresco, E. M. Y., Ehlers, C., Knowlton, K. U., Patapoutian, A., et al. Hypermorphic mutation of the voltage-gated sodium channel encoding gene Scn10a causes a dramatic stimulus-dependent neurobehavioral phenotype Proceedings of the National Academy of Sciences of the United States of America 2011 108:19413-19418 DOI:10.1073/pnas.1117020108 PMID:22087007
- Miyamoto, T., Petrus, M. J., Dubin, A. E., Patapoutian, A. TRPV3 regulates nitric oxide synthase-independent nitric oxide synthesis in the skin Nature Communications 2011 2:369 DOI:10.1038/ncomms1371 PMID:21712817
- Xiao, B. L., Coste, B., Mathur, J., Patapoutian, A. Temperature-dependent STIM1 activation induces Ca(2+) influx and modulates gene expression Nature Chemical Biology 2011 7:351-358 DOI:10.1038/nchembio.558 PMID:21499266
- Dubin, A. E., Patapoutian, A. Nociceptors: The sensors of the pain pathway Journal of Clinical Investigation 2010 120:3760-3772 DOI:10.1172/jci42843 PMID:21041958
- Coste, B., Mathur, J., Schmidt, M., Earley, T. J., Ranade, S., Petrus, M. J., Dubin, A. E., Patapoutian, A. Piezo1 and Piezo2 are essential components of distinct mechanically activated cation channels Science 2010 330:55-60 DOI:10.1126/science.1193270 PMID:20813920
- del Camino, D., Murphy, S., Heiry, M., Barrett, L. B., Earley, T. J., Cook, C. A., Petrus, M. J., Zhao, M., D'Amours, M., Deering, N., Brenner, G. J., Costigan, M., et al. TRPA1 contributes to cold hypersensitivity Journal of Neuroscience 2010 30:15165-15174 DOI:10.1523/jneurosci.2580-10.2010 PMID:21068322
- Grandl, J., Kim, S. E., Uzzell, V., Bursulaya, B., Petrus, M., Bandell, M., Patapoutian, A. Temperature-induced opening of TRPV1 ion channel is stabilized by the pore domain Nature Neuroscience 2010 13:708-714 DOI:10.1038/nn.2552 PMID:20414199
- Schmidt, M., Dubin, A. E., Petrus, M. J., Earley, T. J., Patapoutian, A. Nociceptive signals induce trafficking of TRPA1 to the plasma membrane Neuron 2009 64:498-509 DOI:10.1016/j.neuron.2009.09.030 PMID:19945392
- Miyamoto, T., Dubin, A. E., Petrus, M. J., Patapoutian, A. TRPV1 and TRPA1 mediate peripheral nitric oxide-induced nociception in mice PLoS One 2009 4:e7596 DOI:10.1371/journal.pone.0007596 PMID:19893614
- Dhaka, A., Uzzell, V., Dubin, A. E., Mathur, J., Petrus, M., Bandell, M., Patapoutian, A. TRPV1 is activated by both acidic and basic pH Journal of Neuroscience 2009 29:153-158 DOI:10.1523/jneurosci.4901-08.2009 PMID:19129393
- Mandadi, S., Sokabe, T., Shibasaki, K., Katanosaka, K., Mizuno, A., Moqrich, A., Patapoutian, A., Fukumi-Tominaga, T., Mizumura, K., Tominaga, M. TRPV3 in keratinocytes transmits temperature information to sensory neurons via ATP Pflugers Archiv-European Journal of Physiology 2009 458:1093-1102 DOI:10.1007/s00424-009-0703-x PMID:19669158
- Patapoutian, A., Tate, S., Woolf, C. J. Transient receptor potential channels: Targeting pain at the source Nature Reviews Drug Discovery 2009 8:55-68 DOI:10.1038/nrd2757 PMID:19116627
- Hu, H. Z., Grandl, J., Bandell, M., Petrus, M., Patapoutian, A. Two amino acid residues determine 2-APB sensitivity of the ion channels TRPV3 and TRPV4 Proceedings of the National Academy of Sciences of the United States of America 2009 106:1626-1631 DOI:10.1073/pnas.0812209106 PMID:19164517
- Hu, H. Z., Bandell, M., Petrus, M. J., Zhu, M. X., Patapoutian, A. Zinc activates damage-sensing TRPA1 ion channels Nature Chemical Biology 2009 5:183-190 DOI:10.1038/nchembio.146 PMID:19202543
- Xiao, B., Dubin, A. E., Bursulaya, B., Viswanath, V., Jegla, T. J., Patapoutian, A. Identification of transmembrane domain 5 as a critical molecular determinant of menthol sensitivity in mammalian TRPA1 channels Journal of Neuroscience 2008 28:9640-9651 DOI:10.1523/jneurosci.2772-08.2008 PMID:18815250
- Grandl, J., Hu, H. Z., Bandell, M., Bursulaya, B., Schmidt, M., Petrus, M., Patapoutian, A. Pore region of TRPV3 ion channel is specifically required for heat activation Nature Neuroscience 2008 11:1007-1013 DOI:10.1038/nn.2169 PMID:19160498
- Dhaka, A., Earley, T. J., Watson, J., Patapoutian, A. Visualizing cold spots: TRPM8-expressing sensory neurons and their projections Journal of Neuroscience 2008 28:566-575 DOI:10.1523/jneurosci.3976-07.2008 PMID:18199758
- Petrus, M., Peier, A. M., Bandell, M., Hwang, S. W., Huynh, T., Olney, N., Jegla, T., Patapoutian, A. A role of TRPA1 in mechanical hyperalgesia is revealed by pharmacological inhibition Molecular Pain 2007 3:40 DOI:10.1186/1744-8069-3-40 PMID:18086313
- Macpherson, L. J., Xiao, B., Kwan, K. Y., Petrus, M. J., Dubin, A. E., Hwang, S., Cravatt, B., Corey, D. P., Patapoutian, A. An ion channel essential for sensing chemical damage Journal of Neuroscience 2007 27:11412-11415 DOI:10.1523/jneurosci.3600-07.2007 PMID:17942735
- Kindt, K. S., Viswanath, V., Macpherson, L., Quast, K., Hu, H. Z., Patapoutian, A., Schafer, W. R. Caenorhabditis elegans TRPA-1 functions in mechanosensation Nature Neuroscience 2007 10:568-577 DOI:10.1038/nn1886 PMID:17450139
- Bandell, M., Macpherson, L. J., Patapoutian, A. From chills to chilis: Mechanisms for thermosensation and chemesthesis via thermoTRPs Current Opinion in Neurobiology 2007 17:490-497 DOI:10.1016/j.conb.2007.07.014 PMID:17706410
- Macpherson, L. J., Dubin, A. E., Evans, M. J., Marr, F., Schultz, P. G., Cravatt, B. F., Patapoutian, A. Noxious compounds activate TRPA1 ion channels through covalent modification of cysteines Nature 2007 445:541-545 DOI:10.1038/nature05544 PMID:17237762
- Dhaka, A., Murray, A. N., Mathur, J., Earley, T. J., Petrus, M. J., Patapoutian, A. TRPM8 is required for cold sensation in mice Neuron 2007 54:371-378 DOI:10.1016/j.neuron.2007.02.024 PMID:17481391
- Saghatelian, A., McKinney, M. K., Bandell, M., Patapoutian, A., Cravatt, B. F. A FAAH-regulated class of N-acyl taurines that activates TRP ion channels Biochemistry 2006 45:9007-9015 DOI:10.1021/bi0608008 PMID:16866345
- McCleverty, C. J., Koesema, E., Patapoutian, A., Lesley, S. A., Kreusch, A. Crystal structure of the human TRPV2 channel ankyrin repeat domain Protein Science 2006 15:2201-2206 DOI:10.1110/ps.062357206 PMID:16882997
- Bandell, M., Dubin, A. E., Petrus, M. J., Orth, A., Mathur, J., Hwang, S. W., Patapoutian, A. High-throughput random mutagenesis screen reveals TRPM8 residues specifically required for activation by menthol Nature Neuroscience 2006 9:493-500 DOI:10.1038/nn1665 PMID:16520735
- Macpherson, L. J., Hwang, S. W., Miyamoto, T., Dubin, A. E., Patapoutian, A., Story, G. M. More than cool: Promiscuous relationships of menthol and other sensory compounds Molecular and Cellular Neuroscience 2006 32:335-343 DOI:10.1016/j.mcn.2006.05.005 PMID:16829128
- Moqrich, A., Hwang, S. W., Earley, T. J., Petrus, M. J., Murray, A. N., Spencer, K. S. R., Andahazy, M., Story, G. M., Patapoutian, A. Impaired thermosensation in mice lacking TRPV3, a heat and camphor sensor in the skin Science 2005 307:1468-1472 DOI:10.1126/science.1108609 PMID:15746429
- Patapoutian, A. TRP channels and thermosensation Chemical Senses 2005 30:i193-i194 DOI:10.1093/chemse/bjh180 PMID:15738110
- Rosenzweig, M., Brennan, K. M., Tayler, T. D., Phelps, P. O., Patapoutian, A., Garrity, P. A. The Drosophila ortholog of vertebrate TRPA1 regulates thermotaxis Genes & Development 2005 19:419-424 DOI:10.1101/gad.1278205 PMID:15681611
- Macpherson, L. J., Geierstanger, B. H., Viswanath, V., Bandell, M., Eid, S. R., Hwang, S., Patapoutian, A. The pungency of garlic: Activation of TRPA1 and TRPV1 in response to allicin Current Biology 2005 15:929-934 DOI:10.1016/j/cub.2005.04.018 PMID:15916949
- Moqrich, A., Earley, T. J., Watson, J., Andahazy, M., Backus, C., Martin-Zanca, D., Wright, D. E., Reichardt, L. F., Patapoutian, A. Expressing TrkC from the TrkA locus causes a subset of dorsal root ganglia neurons to switch fate Nature Neuroscience 2004 7:812-818 DOI:10.1038/nn1283 PMID:15247919
- Bandell, M., Story, G. M., Hwang, S. W., Viswanath, V., Eid, S. R., Petrus, M. J., Earley, T. J., Patapoutian, A. Noxious cold ion channel TRPA1 is activated by pungent compounds and bradykinin Neuron 2004 41:849-857 DOI:10.1016/s0896-6273(04)00150-3 PMID:15046718
- Story, G. M., Peier, A. M., Reeve, A. J., Eid, S. R., Mosbacher, J., Hricik, T. R., Earley, T. J., Hergarden, A. C., Andersson, D. A., Hwang, S. W., McIntyre, P., Jegla, T., et al. ANKTM1, a TRP-like channel expressed in nociceptive neurons, is activated by cold temperatures Cell 2003 112:819-829 DOI:10.1016/s0092-8674(03)00158-2 PMID:12654248
- Patapoutian, A., Peier, A. M., Story, G. M., Viswanath, V. ThermoTRP channels and beyond: Mechanisms of temperature sensation Nature Reviews Neuroscience 2003 4:529-539 DOI:10.1038/nrn1141 PMID:12838328
- Peier, A. M., Moqrich, A., Hergarden, A. C., Reeve, A. J., Andersson, D. A., Story, G. M., Earley, T. J., Dragoni, I., McIntyre, P., Bevan, S., Patapoutian, A. A TRP channel that senses cold stimuli and menthol Cell 2002 108:705-715 DOI:10.1016/s0092-8674(02)00652-9 PMID:11893340
- Peier, A. M., Reeve, A. J., Andersson, D. A., Moqrich, A., Earley, T. J., Hergarden, A. C., Story, G. M., Colley, S., Hogenesch, J. B., McIntyre, P., Bevan, S., Patapoutian, A. A heat-sensitive TRP channel expressed in keratinocytes Science 2002 296:2046-2049 DOI:10.1126/science.1073140 PMID:12016205
- Su, A. I., Cooke, M. P., Ching, K. A., Hakak, Y., Walker, J. R., Wiltshire, T., Orth, A. P., Vega, R. G., Sapinoso, L. M., Moqrich, A., Patapoutian, A., Hampton, G. M., et al. Large-scale analysis of the human and mouse transcriptomes Proceedings of the National Academy of Sciences of the United States of America 2002 99:4465-4470 DOI:10.1073/pnas.012025199 PMID:11904358
- Patapoutian, A., Reichardt, L. F. Roles of Wnt proteins in neural development and maintenance Current Opinion in Neurobiology 2000 10:392-399 DOI:10.1016/s0959-4388(00)00100-8 PMID:10851180
- Patapoutian, A., Yoon, J. K., Miner, J. H., Wang, S. L., Stark, K., Wold, B. Disruption of the mouse MRF4 gene identifies multiple waves of myogenesis in the myotome Development 1995 121:3347-3358 PMID:7588068
- Patapoutian, A., Wold, B. J., Wagner, R. A. Evidence for developmentally programmed transdifferentiation in mouse esophageal muscle Science 1995 270:1818-1821 DOI:10.1126/science.270.5243.1818 PMID:8525375
- Patapoutian, A., Miner, J. H., Lyons, G. E., Wold, B. Isolated sequences from the linked Myf-5 and MRF4 genes drive distinct patterns of muscle-specific expression in transgenic mice Development 1993 118:61-69 PMID:8375340
- Verma, R., Patapoutian, A., Gordon, C. B., Campbell, J. L. Identification and purification of a factor that binds to the Mlu I cell cycle box of yeast DNA replication genes Proceedings of the National Academy of Sciences of the United States of America 1991 88:7155-7159 DOI:10.1073/pnas.88.16.7155 PMID:1871128
- Pignoni, F., Baldarelli, R. M., Steingrimsson, E., Diaz, R. J., Patapoutian, A., Merriam, J. R., Lengyel, J. A. The Drosophila gene tailless is expressed at the embryonic termini and is a member of the steroid receptor superfamily Cell 1990 62:151-163 DOI:10.1016/0092-8674(90)90249-e PMID:2364433
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Research
research overview
- The sense of touch consists of the perception of discrete types of thermal, mechanical, and chemical stimuli. However, little is known about the molecular basis of touch perception. Our lab is characterizing the genes involved in the first step of touch sensation: those that encode the molecular sensors of touch stimuli. With the completion of the human genome project, we have powerful new methods to identify these elusive sensory molecules. We recently identified the first gene involved in our ability to sense cold temperatures. This gene, trpm8, encodes for a protein present at the plasma membrane of cold-sensing neurons that belongs to the Transient Receptor Potential (TRP) channel family. Interestingly, TRPM8 is also activated by menthol, a commonly-used cooling compound. We are continuing to identify additional sensory receptors, and working towards understanding the mechanism of activation of these channels. Another direction in the lab is to understand how these sensory neurons become specialized during development. We are using transgenic and genomic technologies to address these questions.
principal investigator on
- A metabolomic search for endogenous chemical agonists of nociceptive TRP channels awarded by National Institute of Dental and Craniofacial Research 2010 - 2012
- Identification of novel somatosensory receptors awarded by National Institute of Dental and Craniofacial Research 2011 - 2015
- Role of STIM1 in somatosensation awarded by National Institute of Dental and Craniofacial Research 2012 - 2014
- Role of mechanically activated ion channels in somatosensation awarded by National Institute of Dental and Craniofacial Research 2012 - 2017
- Structure function of mechanically activated ion channel awarded by National Institute of Neurological Disorders and Stroke 2012 - 2017
Background
education and training
- Ph.D. in Biology, California Institute of Technology, with Dr. Barbara Wold 1990 - 1996
- B.S. in Molecular, Cellular & Development Biology, University of California, Los Angeles, magna cum laude, with Dr. Judy A. Lengyel 1987 - 1990