- Gruol Laboratory Associate Professor
Cellular and Molecular Mechanisms of Neuronal Signaling in the CNS
The research interests of this laboratory center on the pathways and mechanisms involved in neuronal signaling in the mammalian central nervous system (CNS), developmental expression of signaling pathways, and the neuroadaptive mechanisms through which CNS disease and drugs alter neuronal signaling and viability. To address these issues we use electrophysiological techniques (whole cell current clamp, voltage clamp and single channel recordings), fura-2 based microscopic imaging of intracellular calcium, biochemical and gene array analyses, immunohistochemical techniques and in vitro model systems (primary cultures of CNS neurons and acutely isolated brain slices). Current research projects include: (a) identification of neuroadaptive mechanisms involved in CNS actions of cytokines and chemokines important in CNS disease, (b) pathways involved in intracellular calcium signaling in CNS neurons, (c) the physiological role of cannabinoid receptors in CNS neuronal function, and (d) the mechanisms involved in neuroadaption to chronic alcohol exposure at synaptic pathways in the hippocampus.